LOCUS       sequence-284574-        1514 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1514)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1514)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2701
FEATURES             Location/Qualifiers
     source          1..1514
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             219..230
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 accgccacca tgtggcgaga atgtgattgg ggtcttggag cagtcaagtc tgatctggcc
       61 tgtgttcctt cagctaaaag gcttctctgc agaatgtcaa acaaagatcg acacattgat
      121 tccagctgtt cgtccttcat caagacggaa ccttccagcc cagcctccct gacggacagc
      181 gtcaaccacc acagccctgg tggctcttca gacgccagtg ggagctacag ttcaaccatg
      241 aatggccatc agaacggact tgactcgcca cctctctacc cttctgctcc tatcctggga
      301 ggtagtgggc ctgtcaggaa actgtatgat gactgctcca gcaccattgt tgaagatccc
      361 cagaccaagt gtgaatacat gctcaactcg atgcccaaga gactgtgttt agtgtgtggt
      421 gacatcgctt ctgggtacca ctatggggta gcatcatgtg aagcctgcaa ggcattcttc
      481 aagaggacaa ttcaaggcaa tatagaatac agctgccctg ccacgaatga atgtgaaatc
      541 acaaagcgca gacgtaaatc ctgccaggct tgccgcttca tgaagtgttt aaaagtgggc
      601 atgctgaaag aaggggtgcg tcttgacaga gtacgtggag gtcggcagaa gtacaagcgc
      661 aggatagatg cggagaacag cccatacctg aaccctcagc tggttcagcc agccaaaaag
      721 ccattgctct ggtctgatcc tgcagataac aagattgtct cacatttgtt ggtggctgaa
      781 ccggagaaga tctatgccat gcctgaccct actgtccccg acagtgacat caaagccctc
      841 actacactgt gtgacttggc cgaccgagag ttggtggtta tcattggatg ggcgaagcat
      901 attccaggct tctccacgct gtccctggcg gaccagatga gccttctgca gagtgcttgg
      961 atggaaattt tgatccttgg tgtcgtatac cggtctcttt cgtttgagga tgaacttgtc
     1021 tatgcagacg attatataat ggacgaagac cagtccaaat tagcaggcct tcttgatcta
     1081 aataatgcta tcctgcagct ggtaaagaaa tacaagagca tgaagctgga aaaagaagaa
     1141 tttgtcaccc tcaaagctat agctcttgct aattcagact ccatgcacat agaagatgtt
     1201 gaagccgttc agaagcttca ggatgtctta catgaagcgc tgcaggatta tgaagctggc
     1261 cagcacatgg aagaccctcg tcgagctggc aagatgctga tgacactgcc actcctgagg
     1321 cagacctcta ccaaggccgt gcagcatttc tacaacatca aactagaagg caaagtccca
     1381 atgcacaaac tttttttgga aatgttggag gccaaggtct agtaatgaac tagtccgtaa
     1441 cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga catgcgggat
     1501 tctatgggcg cagg
//
