LOCUS       sequence-28458-e         725 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 725)
  AUTHORS   Garcia-Cardena G, Martasek P, Masters BS, Skidd PM, Couet J, Li S, 
            Lisanti MP, Sessa WC
  TITLE     Dissecting the interaction between nitric oxide synthase (NOS) and 
            caveolin. Functional significance of the nos caveolin binding domain
            in vivo.
  JOURNAL   J Biol Chem. 1997 Oct 10. 272(41):25437-40.
  PUBMED    9325253
REFERENCE   2  (bases 1 to 725)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 1997 Oct 10. 272(41):25437-40."
COMMENT     Sequence Label: GST-Cav (1-61)
FEATURES             Location/Qualifiers
     source          1..725
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..24
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     complement(291..313)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(458..476)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        544..648
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tcggatctgg ttccgcgtgg atccatgtct gggggcaaat acgtagactc cgaggggcac
       61 ctctacaccg ttcccatccg ggagcagggc aacatctaca agcccaacaa caaggccatg
      121 gcggaggaga tgagcgagaa gcaggtgtac gacgcgcaca ccaaggaaat cgacctggtc
      181 aaccgcgacc ccaagcatct caacgactaa gaattcccgg gtcgactcga gcggccgcat
      241 cgtgactgac tgacgatctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac
      301 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc
      361 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg cagccatgac ccagtcacgt
      421 agcgatagcg gagtgtataa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta
      481 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat
      541 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
      601 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
      661 cttttccngt gtcgccctta ttcccttntt ttgcggcatt ttgccttcct tgttttttgc
      721 ctcac
//
