LOCUS       sequence-284583-        1460 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1460)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1460)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2735
FEATURES             Location/Qualifiers
     source          1..1460
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      8..17
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcggccgt ggcgctggcg agcgggacac gcctcggcct tgtcctcgag
       61 ctgctcccgg gacagcccgc gctgccccgg gcgcgccggg agtcagtgac cttcgaggat
      121 gtggccgtct acttctctga gaacgaatgg atcggcctgg gccctgctca gagagccctg
      181 tacagggatg tgatgctgga gaattatggg gctgtggctt ccctggcatt tccatttccc
      241 aaaccggctc tgatttccca gctggagcga ggggaaacac cctggtgctc ggttcctcgg
      301 ggagctctgg atggagaggc cccaaggggc atctcctcag agggtgtgtt gaagaggaag
      361 aaagaagatt ttattctgaa ggaggaaatt attgaggaag cacaggacct catggtccta
      421 tcaagtggac cccagtggtg tggatcccag gaattatggt ttgggaaaac ctgtgaagag
      481 aaaagcaggt tagggagatg gcctggttac ctcaatgggg gacgtatgga aagttctaca
      541 aatgatatta tagaagtgat tgtcaaggat gagatgatct cagtagaaga gagttcaggg
      601 aatactgatg tcaataacct ccttggtata catcacaaaa ttctaaatga gcaaatattc
      661 tatatatgtg aggaatgcgg caagtgtttt gatcaaaatg aggactttga tcaacaccag
      721 aaaactcata atggagagaa ggtctatgga tgtaaggaat gtgggaaggc tttcagtttt
      781 cgatcacatt gcattgcaca tcagagaatt cacagtgggg tgaaacccta tgaatgtcaa
      841 gaatgtgcta aggcctttgt ttggaagtca aacctgattc gtcaccagag aatacatact
      901 ggagagaaac cctttgaatg taaggaatgt gggaagggct ttagtcagaa cacaagcctt
      961 acgcaacatc aacggatcca cactggtgag aaaccataca catgtaagga atgtgggaaa
     1021 agctttactc gaaacccagc ccttcttcga catcagagaa tgcacactgg ggagaagcct
     1081 tacgaatgta aggactgtgg gaagggcttc atgtggaact cagatctttc tcagcaccag
     1141 agggtccaca ctggggacaa gcctcatgaa tgtactgact gtgggaaaag cttcttttgc
     1201 aaggcacatc ttattcgaca tcaaagaatc catactgggg aaagacccta taaatgtaat
     1261 gactgtggga aggccttcag tcagaattct gtcttaatta agcaccagag gcgccatgct
     1321 agagacaaac cctataactg tcagatctct caccttcttg aacattagta atgaactagt
     1381 ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa ggacgaggtg
     1441 acaaatctga ggataatggt
//
