LOCUS       sequence-284586-        3014 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3014)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 3014)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2737
FEATURES             Location/Qualifiers
     source          1..3014
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1740..1749
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcgacctg catcggggag aagatcgagg attttaaagt tggaaatctg
       61 cttggtaaag gatcatttgc tggtgtctac agagctgagt ccattcacac tggtttggaa
      121 gttgcaatca aaatgataga taagaaagcc atgtacaaag caggaatggt acagagagtc
      181 caaaatgagg tgaaaataca ttgccaattg aaacatcctt ctatcttgga gctttataac
      241 tattttgaag atagcaatta tgtgtatctg gtattagaaa tgtgccataa tggagaaatg
      301 aacaggtatc taaagaatag agtgaaaccc ttctcagaaa atgaagctcg acacttcatg
      361 caccagatca tcacagggat gttgtatctt cattctcatg gtatactaca ccgggacctc
      421 acactttcta acctcctact gactcgtaat atgaacatca agattgctga ttttgggctg
      481 gcaactcaac tgaaaatgcc acatgaaaag cactatacat tatgtggaac tcctaactac
      541 atttcaccag aaattgccac tcgaagtgca catggccttg aatctgatgt ttggtccctg
      601 ggctgtatgt tttatacatt acttatcggg agaccaccct tcgacactga cacagtcaag
      661 aacacattaa ataaagtagt attggcagat tatgaaatgc catctttttt gtcaatagag
      721 gccaaggacc ttattcacca gttacttcgt agaaatccag cagatcgttt aagtctgtct
      781 tcagtattgg accatccttt tatgtcccga aattcttcaa caaaaagtaa agatttagga
      841 actgtggaag actcaattga tagtgggcat gccacaattt ctactgcaat tacagcttct
      901 tccagtacca gtataagtgg tagtttattt gacaaaagaa gacttttgat tggtcagcca
      961 ctcccaaata aaatgactgt atttccaaag aataaaagtt caactgattt ttcttcttca
     1021 ggagatggaa acagttttta tactcagtgg ggaaatcaag aaaccagtaa tagtggaagg
     1081 ggaagagtaa ttcaagatgc agaagaaagg ccacattctc gataccttcg tagagcttat
     1141 tcctctgata gatctggcac ttctaatagt cagtctcaag caaaaacata tacaatggaa
     1201 cgatgtcact cagcagaaat gctttcagtg tccaaaagat caggaggagg tgaaaatgaa
     1261 gagaggtact cacccacaga caacaatgcc aacattttta acttctttaa agaaaagaca
     1321 tccagtagtt ctggatcttt tgaaagacct gataacaatc aagcactctc caatcatctt
     1381 tgtccaggaa aaactccttt tccatttgca gacccgacac ctcagactga aaccgtacaa
     1441 cagtggtttg ggaatctgca aataaatgct catttaagaa aaactactga atatgacagc
     1501 atcagcccaa accgggactt ccagggccat ccagatttgc agaaggacac atcaaaaaat
     1561 gcctggactg atacaaaagt caaaaagaac tctgatgctt ctgataatgc acattctgta
     1621 aaacagcaaa ataccatgaa atatatgact gcacttcaca gtaaacctga gataatccaa
     1681 caagaatgtg tttttggctc agatcctctt tctgaacaga gcaagactag gggtatggag
     1741 ccaccatggg gttatcagaa tcgtacatta agaagcatta catctccgtt ggttgctcac
     1801 aggttaaaac caatcagaca gaaaaccaaa aaggctgtgg tgagcatact tgattcagag
     1861 gaggtgtgtg tggagcttgt aaaggagtat gcatctcaag aatatgtgaa agaagttctt
     1921 cagatatcta gtgatggaaa tacgatcact atttattatc caaatggtgg tagaggtttt
     1981 cctcttgctg atagaccacc ctcacctact gacaacatca gtaggtacag ctttgacaat
     2041 ttaccagaaa aatactggcg aaaatatcaa tatgcttcca ggtttgtaca gcttgtaaga
     2101 tctaaatctc ccaaaatcac ttattttaca agatatgcta aatgcatttt gatggagaat
     2161 tctcctggtg ctgattttga ggtttggttt tatgatgggg taaaaataca caaaacagaa
     2221 gatttcattc aggtgattga aaagacaggg aagtcttaca ctttaaaaag tgaaagtgaa
     2281 gttaatagct tgaaagagga gataaaaatg tatatggacc atgctaatga gggtcatcgt
     2341 atttgtttag cactggaatc cataatttca gaagaggaaa ggaaaactag gagtgctccc
     2401 tttttcccaa taatcatagg aagaaaacct ggtagtacta gttcacctaa ggccttatca
     2461 cctcctcctt ctgtggattc aaattaccca acgagagaga gagcatcttt caacagaatg
     2521 gtcatgcata gtgctgcttc tccaacacag gcaccaatcc ttaatccctc tatggttaca
     2581 aatgaaggac ttggtcttac aactacagct tctggaacag acatctcttc taatagtcta
     2641 aaagattgtc ttcctaaatc agcacaactt ttgaaatctg tttttgtgaa aaatgttggt
     2701 tgggctacac agttaactag tggagctgtg tgggttcagt ttaatgatgg gtcccagttg
     2761 gttgtgcagg caggagtgtc ttctatcagt tatacctcac caaatggtca aacaactagg
     2821 tatggagaaa atgaaaaatt accagactac atcaaacaga aattacagtg tctgtcttcc
     2881 atccttttga tgttttctaa tccgactcct aattttcatt agtaatgaac tagtccgtaa
     2941 cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga gcaacgactt
     3001 gctcggttct gaac
//
