LOCUS       sequence-28459-e         687 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 687)
  AUTHORS   Garcia-Cardena G, Martasek P, Masters BS, Skidd PM, Couet J, Li S, 
            Lisanti MP, Sessa WC
  TITLE     Dissecting the interaction between nitric oxide synthase (NOS) and 
            caveolin. Functional significance of the nos caveolin binding domain
            in vivo.
  JOURNAL   J Biol Chem. 1997 Oct 10. 272(41):25437-40.
  PUBMED    9325253
REFERENCE   2  (bases 1 to 687)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 1997 Oct 10. 272(41):25437-40."
COMMENT     Sequence Label: GST-Cav (61-101)
FEATURES             Location/Qualifiers
     source          1..687
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             6..23
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     complement(230..252)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(397..415)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        483..587
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cggatctggt tccgcgtgga tccgacgacg tggtcaagat tgattttgaa gatgtgattg
       61 cagaaccaga aggaacacac agttttgatg gcatctggaa ggccagcttc accaccttca
      121 ctgtgacaaa atactggttt taccgctaag aattcccggg tcgactcgag cggccgcatc
      181 gtgactgact gacgatctgc ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca
      241 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc
      301 gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc cagtcacgta
      361 gcgatagcgg agtgtataat tcttgaagac gaaagggcct cgtgatacgc ctatttttat
      421 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
      481 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
      541 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
      601 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
      661 cagaaaccgc tggtgaaagt aaaagat
//
