LOCUS       sequence-284598-        5984 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5984)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 5984)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2847
FEATURES             Location/Qualifiers
     source          1..5984
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 accgccacca tgcagtcctt tcgggagcaa agcagttacc acggaaacca gcaaagctac
       61 ccacaggagg tacacggctc atcccggcta gaagagttca gccctcgtca ggcccagatg
      121 ttccagaatt ttggaggtac aggtggcagt agtggcagca gtggcagtgg cagtggtggt
      181 ggacgacgag gagcagcagc tgctgcggca gcgatggcta gcgagacctc tggccatcaa
      241 ggttaccagg gtttcaggaa agaggctgga gatttttact acatggcagg caacaaagac
      301 cccgtgacta caggaacccc acagcctcct cagcgaaggc cttctgggcc tgtgcagagc
      361 tatggacccc cccaggggag cagctttggc aatcagtatg ggagtgaggg tcatgtgggc
      421 cagtttcaag cacagcactc tggccttggc ggtgtgtcac attatcagca ggattacact
      481 gggcctttct ctccagggag tgctcagtac caacagcagg cttccagcca gcagcagcag
      541 cagcaagtcc agcagttgag acaacagctt taccagtccc atcagcccct gccacaggcc
      601 actggccaac cagcatccag ctcatcccat ctacagccaa tgcagcggcc ctcaactctg
      661 ccatcctctg ctgctggtta ccagttaaga gtgggtcagt ttggccaaca ctatcagtct
      721 tctgcttcct cctcctcctc ctcctccttc ccttcaccac agcgttttag ccagtctgga
      781 cagagctatg atggcagtta caatgtgaat gctggatctc agtatgaagg acacaatgtg
      841 ggttctaatg cacaggctta tggaacacaa tccaattaca gctatcagcc tcaatctatg
      901 aagaattttg aacaggcaaa gattccacaa gggacccaac aggggcagca gcagcagcaa
      961 ccgcagcaac aacaacaccc ttctcagcat gtgatgcagt atactaacgc tgccaccaag
     1021 ctgcccctgc aaagccaagt ggggcagtac aaccagcctg aggttcctgt gaggtccccc
     1081 atgcagtttc accagaactt cagccccatt tctaaccctt ctccagctgc ctctgtggtt
     1141 cagtctccaa gctgtagttc taccccatct cctctcatgc agactgggga gaatctccag
     1201 tgtgggcaag gcagtgtgcc tatgggttcc agaaacagaa ttttacagtt aatgcctcaa
     1261 ctcagtccaa ccccatcaat gatgcccagt cctaattctc atgctgcagg cttcaaaggg
     1321 tttggactag aaggggtacc agaaaagcga ctgacagatc ctgggttgag tagtttgagt
     1381 gctctgagta ctcaagtggc caatcttcct aacactgtcc agcacatgtt actttctgat
     1441 gccctgactc ctcagaagaa gacctccaag aggccctcat cttccaagaa agcagatagc
     1501 tgcacaaatt ctgaaggctc ctcacaacct gaagaacagc tgaagtcccc tatggcagag
     1561 tcattagatg gaggctgctc cagcagttca gaggatcaag gcgagagagt gcggcaacta
     1621 agtggccaga gcaccagctc tgacaccacc tacaagggtg gagcctctga gaaagctggc
     1681 tcctcaccgg cacaaggtgc tcagaatgaa ccccccagac tcaatgctag tcctgccgca
     1741 agagaagagg ccacctcacc aggcgctaag gacatgccat tgtcatccga cgggaaccca
     1801 aaggttaatg agaagactgt tggggtgatt gtctcccggg aagccatgac aggtcgggta
     1861 gaaaagcctg gtggacaaga taaaggctcc caagaggatg atcctgcagc cactcaaagg
     1921 ccacctagca atggtggggc aaaggaaacc agtcatgcat cacttcccca gccagagcct
     1981 ccaggaggag gagggagcaa aggaaacaag aatggcgata acaactccaa ccataatgga
     2041 gaaggaaatg gccagagtgg ccactctgca gcgggccctg gttttacgag cagaactgag
     2101 cctagcaaat ctcctggaag tctgcgctat agttacaaag atagtttcgg gtcagccgtg
     2161 ccacgaaatg tcagtggctt tcctcagtat cctacagggc aagaaaaggg agatttcact
     2221 ggccatgggg aacgaaaggg tagaaatgaa aaattcccaa gcctcctgca ggaagtgctt
     2281 cagggttacc accaccaccc tgacaggaga tattctagga gtactcaaga gcatcagggg
     2341 atggctggta gcctagaagg aaccacaagg cccaatgtct tggttagtca aaccaatgaa
     2401 ttagctagca ggggccttct gaacaaaagc attgggtctc tattagaaaa tccccactgg
     2461 ggcccctggg aaaggaaatc aagcagcaca gctcctgaaa tgaaacagat caatttgact
     2521 gactatccaa ttcccagaaa gtttgaaata gagcctcagt catcagcaca tgagcctggg
     2581 ggttccctct ctgaaagaag atcagtgatc tgtgatattt ctccactaag acagattgtc
     2641 agggacccag gggctcactc actgggacac atgagtgccg acaccagaat tgggaggaat
     2701 gaccgtctca atccaacttt aagtcagtcg gtcattcttc ctggtggttt ggtgtccatg
     2761 gaaaccaagc tgaaatccca gagcgggcag ataaaagagg aagactttga acagtctaaa
     2821 tctcaagcta gtttcaacaa caagaaatct ggagaccact gccatcctcc tagcatcaag
     2881 catgagtctt accgcggcaa tgccagccct ggagcagcaa cccatgattc cctttcagac
     2941 tatggcccgc aagacagcag acccacgcca atgcggcggg tccctggcag agttggtggt
     3001 cgggagggca tgaggggtcg gtccccttct caatatcatg actttgcaga aaaattgaaa
     3061 atgtctcctg ggcggagcag aggcccaggg ggagaccctc atcacatgaa tccacacatg
     3121 accttttcag agagggctaa ccggagttct ttacacactc ccttttctcc caactcagaa
     3181 accctggcct ctgcttatca tgcaaatact cgggctcatg cttatgggga ccctaacgca
     3241 ggtttgaatt ctcagctgca ttataagaga cagatgtacc aacagcaacc agaggagtat
     3301 aaagactgga gcagcggttc tgctcaggga gtaattgctg cagcacagca caggcaggag
     3361 gggccacgga agagtccaag gcagcagcag tttcttgaca gagtacggag ccctctgaaa
     3421 aatgacaaag atggtatgat gtatggccca ccagtgggga cttaccatga ccccagtgcc
     3481 caggaggctg ggcgctgcct aatgtctagt gatggtctgc ctaacaaggg catggaatta
     3541 aagcatggct cccagaagtt acaagaatcc tgttgggatc tttctcggca aacttctcca
     3601 gccaaaagca gcggtcctcc aggaatgtcc agtcaaaaaa ggtatgggcc gccccatgag
     3661 actgatggac atggactagc tgaggctaca cagtcatcca aacctggtag tgttatgctg
     3721 agacttccag gccaggagga tcattcttct caaaacccct taatcatgag gaggcgtgtt
     3781 cgttctttta tctctcccat tcccagtaag agacagtcac aagatgtaaa gaacagtagc
     3841 actgaagata aaggtcgcct ccttcactca tcaaaagaag gcgctgataa agcattcaat
     3901 tcctatgccc atctttctca cagtcaggat atcaagtcta tccctaagag agattcctcc
     3961 aaggaccttc caagtccaga tagtagaaac tgccctgctg ttaccctcac aagccctgct
     4021 aagaccaaaa tactgccccc acggaaagga cggggattga aattggaagc tatagttcag
     4081 aagattacat ccccaaatat taggaggagc gcatcttcga acagtgcgga ggctggggga
     4141 gacacggtta cgcttgatga tatactgtct ttgaagagtg gtcctcctga aggtgggagt
     4201 gttgctgttc aggatgctga catagagaag agaaaaggtg aggtggcttc ggacctagtc
     4261 agtccagcaa accaggagtt gcacgtagag aaacctcttc caaggtcttc agaagagtgg
     4321 cgtggcagcg tggatgacaa agtgaagaca gagacacatg cagaaacagt tactgccgga
     4381 aaggaacccc ctggtgccat gacatccaca acctcacaga agcctggtag taaccaaggg
     4441 agaccagatg gttccctggg tggaacagca cctttaatct ttccagactc aaagaatgta
     4501 cctccagtgg gcatattggc ccctgaggca aaccccaagg ctgaagagaa ggagaacgat
     4561 acagtgacga tttcaccgaa gcaagagggt ttccctccaa agggatattt cccatcagga
     4621 aagaagaagg ggagacccat tggtagtgtg aataagcaaa agaaacagca gcagccaccg
     4681 cctccacccc ctcagccccc acagatacca gaaggttctg cagatggaga gccaaagcca
     4741 aaaaaacaga ggcaaaggag ggagagaagg aagcctgggg cccagccgag gaagcgaaaa
     4801 accaaacaag cagttcccat tgtggaaccc caagaacctg agatcaaact aaaatatgcc
     4861 acccagccac tggataaaac tgatgccaag aacaagtctt tttaccctta catccatgta
     4921 gtaaataagt gtgaacttgg agccgtttgt acaatcatca atgctgagga agaagaacag
     4981 accaaattag tgaggggcag gaagggtcag aggtcactga cccctccacc tagcagcact
     5041 gaaagcaagg cgctcccggc ctcgtccttt atgctgcagg gacctgttgt gacagagtct
     5101 tcggttatgg ggcacctggt ttgctgtctg tgtggcaagt gggccagtta ccggaacatg
     5161 ggtgacctct ttggaccttt ttatccccaa gattatgcag ccactctccc gaagaatcca
     5221 cctcctaaga gggccacaga aatgcagagc aaagttaagg tacggcacaa aagtgcttct
     5281 aatggctcca agacggacac tgaggaggag gaagagcagc agcagcagca gaaggagcag
     5341 agaagcctgg ccgcacaccc caggtttaag cggcgccacc gctcggaaga ctgtggtgga
     5401 ggccctcggt ccctgtccag ggggctccct tgtaaaaaag cagccactga gggcagcagt
     5461 gaaaagactg ttttggactc gaagccctcc gtgcccacca cttcagaagg tggccctgag
     5521 ctggagttac aaatccctga actacctctt gacagcaatg aattttgggt ccatgagggt
     5581 tgtattctct gggccaatgg aatctacctg gtttgtggca ggctctatgg cctgcaggaa
     5641 gcgctggaaa tagccagaga gatgaaatgt tcccactgcc aggaggcagg cgccaccttg
     5701 ggctgctaca acaaaggctg ctccttccga taccattacc cgtgtgccat tgatgcagat
     5761 tgtttgctac atgaggagaa cttctcggtg aggtgcccta agcacaagcc tccccttccg
     5821 tgccctctcc cccccttgca gaacaagacc gcgaaaggca gcctcagcac agagcagtcg
     5881 gagcgggggt agtaatgaac tagtccgtaa cttgaaagta tttcgatttc ttggctttat
     5941 atatcttgtg gaaaggacga acttagacga aagacatatc catc
//
