LOCUS       sequence-284604-        3281 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3281)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 3281)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2869
FEATURES             Location/Qualifiers
     source          1..3281
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggaggcgaa cccagcgggc agcggcgccg ggggtggcgg gagcagcggc
       61 atcgggggcg aggacggggt gcacttccag agctacccct tcgacttcct ggaattcctc
      121 aaccaccagc gcttcgagcc catggaactg tatggggaac acgccaaggc ggtggcggcc
      181 ctgccctgcg cccccggccc cccgccgcag cccccgccgc agccccctcc cccgcagtat
      241 gactacccgc cccagtccac cttcaagccc aaggcggagg tgccctcctc gtcctcgtcc
      301 tcgtcctcct cctcctcctc ttcgtcctcc tcgtcgtcat cttcgtcctc ttcctcttcc
      361 caagccaaga agcccgatcc gcccctgccg cccgccttcg gggcgccccc tcctcccctc
      421 tttgacgctg ctttccccac tccgcagtgg ggcatcgtgg acctctcggg gcaccagcac
      481 ttgtttggga acctgaagcg aggagggccc gcgtccgggc cgggggtgac gcctgggctg
      541 ggcgctcccg cgggggcccc agggccgctt cctgccccct cgcagacccc gccaggaccc
      601 cccgcggcgg cggcctgcga ccccaccaag gacgacaagg gctacttccg gagactgaag
      661 tacctgatgg agcggcgctt cccctgcggc gtgtgccaga agtccttcaa gcagtcctcg
      721 cacctggtcc agcacatgct ggtgcactcg ggggagaggc cctacgaatg cggcgtctgc
      781 ggccgcacct acaaccacgt gtccagcctc atccgccacc gccgctgcca caaggacgtg
      841 ccaccggccg cggggggccc gccccagccc ggcccccacc tcccgccgct gggcctccca
      901 gcacccgctg ccagcgccgc caccgccgcc gccccctcca cggtgtcctc gggccctcca
      961 gccacgcccg tggcgcctgc cccctccgca gacgggagcg ccgcccctgc tggtgttggg
     1021 gtgccccctc ctgccaccgg gggtggcgat ggcccgttcg cctgcccact ctgctggaag
     1081 gttttcaaga agcccagtca cctccaccag caccagatca tccacacggg cgagaagccc
     1141 ttctcctgct ccgtgtgcag caaaagcttc aaccgcaggg agagtctgaa gcgccacgtg
     1201 aagacgcact cggccgacct cctgcgcctg ccctgcggca tctgcgggaa ggccttccgc
     1261 gacgcctcct acctcctcaa gcaccaggcg gcccacgcgg gggcgggcgc cggggggcct
     1321 cggcccgtgt acccctgcga cctgtgcggc aagtcctact cggctccgca gagcctgctc
     1381 cgccacaagg ccgcccacgc cccgcccgct gccgctgcgg aggcgcccaa ggacggggcg
     1441 gcctcggccc cgcagccccc gcccaccttc cccccgggcc cgtacctcct gccccccgac
     1501 cctcccacca cagacagcga gaaggcggcg gcggccgcgg cggcggtggt gtacggcgct
     1561 gtgcccgtcc cgctcctggg cgcccacccg ctgctgctcg gcggcgcggg gaccagcggg
     1621 gcgggaggct cgggcgccag cgtcccagga aagacgttct gctgcggcat ctgcgggcgc
     1681 ggcttcgggc gccgcgagac cctgaagcgc catgagcgca tccacacggg cgagaagccc
     1741 caccagtgcc ccgtgtgtgg gaagcgcttc cgcgaatcct tccacttgag caagcatcac
     1801 gtggtgcaca cgcgcgagcg gccctacaag tgcgagctct gcggcaaggt cttcggctac
     1861 ccgcagagcc tcacccgcca ccgccaggtg caccggctcc agctgccctg cgccctggcc
     1921 ggggcagccg gcctcccctc cacccaaggc acaccggggg cctgtgggcc cggggcctcg
     1981 ggcacgtctg cagggcccac cgatgggctg agctacgcct gctcggactg cggcgagcac
     2041 ttcccggatc tctttcacgt catgagtcac aaggaggtcc acatggcaga gaagccatac
     2101 ggctgcgacg cctgcggcaa gaccttcggc ttcatcgaga acctcatgtg gcacaagctg
     2161 gtccaccagg ccgcccccga gcgcctgctc ccgcccgcac ccggcggcct gcagcccccg
     2221 gacggctcca gcggcacgga tgcggccagc gtgctggaca acgggctggc gggggaggtg
     2281 ggggcggccg tggcggcact ggcaggggtg tctgggggtg aggacgcagg cggggcggcg
     2341 gtggcaggtg ctggcggggg tgccagttcc ggccccgagc gcttcagctg tgccacgtgc
     2401 ggccagagtt tcaagcactt cctgggcctc gtgactcaca agtacgtgca cctggtgcga
     2461 cggaccctgg gctgcggcct ctgcggccag agcttcgcgg gcgcctacga cttgctccta
     2521 caccgccgca gccatcggca gaagcggggt ttccgctgcc cggtgtgcgg gaagcgcttc
     2581 tgggaggcgg ccctgctgat gcgccaccag cgctgccaca cggaacagcg gccgtaccga
     2641 tgtggcgtgt gcggccgagg cttcctgcgc tcctggtacc tgcggcagca ccgcgtggtg
     2701 cacactggcg agcgggcctt caagtgcggc gtgtgcgcca agcgcttcgc gcagtcgtcc
     2761 agcctggcag agcaccggcg gctgcacgct gtggcccggc cccagcgctg cagcgcctgt
     2821 ggcaagacct tccgctaccg ctccaacctg ctggagcacc agcggctgca cctgggcgag
     2881 cgcgcctacc gctgtgagca ctgcggcaag ggcttcttct acctgagctc cgtgctgcgc
     2941 caccagcgcg cccatgagcc gccgcggccc gagctccgct gccccgcctg cctcaaggcc
     3001 ttcaaggatc ccggctactt ccgtaagcac ctggctgccc accagggcgg ccggcccttc
     3061 cgctgctcct cctgcggcga gggcttcgcc aacacctacg gcctcaagaa acaccgcctg
     3121 gcgcacaagg ccgagaacct cggggggcct ggagcagggg cgggcacctt ggccgggaag
     3181 gatgcctagt aatgaactag tccgtaactt gaaagtattt cgatttcttg gctttatata
     3241 tcttgtggaa aggacgaatc ctttccgaga tccgctcggg t
//
