LOCUS       sequence-28461-e         710 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 710)
  AUTHORS   Garcia-Cardena G, Martasek P, Masters BS, Skidd PM, Couet J, Li S, 
            Lisanti MP, Sessa WC
  TITLE     Dissecting the interaction between nitric oxide synthase (NOS) and 
            caveolin. Functional significance of the nos caveolin binding domain
            in vivo.
  JOURNAL   J Biol Chem. 1997 Oct 10. 272(41):25437-40.
  PUBMED    9325253
REFERENCE   2  (bases 1 to 710)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 1997 Oct 10. 272(41):25437-40."
COMMENT     Sequence Label: GST-Cav (135-178)
FEATURES             Location/Qualifiers
     source          1..710
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(22..39)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(46..699)
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     complement(66..88)
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
ORIGIN
        1 tcttcttccc gggaattccg gggatccacg cggaaccaga tccgattttg gaggatggtc
       61 gccaccacca aacgtggctt gccagccctg caaaggccat gctatatact tgctggattt
      121 caagtactta tcaatttgtg ggatagcttc aatacgtttt ttaaaacaaa ctaattttgg
      181 gaacgcatcc aggcacattg ggtccatgta taaaacaaca tcaagagcgt catacaacat
      241 gaagtcagga tgggttacat gatcaccatt taaatatgtt ttatgacata aacgatcttc
      301 gaacattttc agcatttcag gtagcttgct aagaaaatca actttgagag tttcaaagtc
      361 tttactatat gcaattctcg aaacaccgta tctaatatcc aaaaccgctc cttcaagcat
      421 tgaaatctct gcacgctctt ttggacaacc acccaacatg ttgtgcttgt cagctatata
      481 acgtatgatg gccatagact gtgttaattt aacatcacca tcaatataat aaggaagatt
      541 gggaaactcc aaacccaatt caaacttttt gtttcgccat ttatcacctt catcgcgctc
      601 atacaaatgc tcttcatatt tttcttcaag atattccaaa agaagtcgag tgggttgcac
      661 aaggccctta attttccaat aacctagtat aggggacatg aatactgttt
//
