LOCUS       sequence-284646-        1541 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1541)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1541)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1487
FEATURES             Location/Qualifiers
     source          1..1541
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggagcggga cgaaccgccg cctagcggag ggggaggcgg cgggggctcg
       61 gcggggttcc tggagcctcc cgccgcgctc cctccgccgc cgcgcaacgg tttctgtcag
      121 gatgaattgg cagagcttga cccaggcact atttctgttt cagatgatcg ggctgaacaa
      181 cgaacctgtc tcatttgtgg ggaccgcgct acaggcttgc actatgggat catctcctgt
      241 gagggctgca aagggttttt caagcggagc atttgcaaca aacgggtata tcgatgcagt
      301 cgtgacaaga actgtgtcat gtctcggaag cagaggaaca ggtgccagta ctgccgcctg
      361 ctcaaatgcc tccagatggg gatgaaccgg aaggctatca gagaagatgg catgcctgga
      421 ggccggaata agagcattgg gccagtccag atatcggaag aagaaatcga aaggatcatg
      481 tctgggcagg agtttgagga agaggccaat cactggagca accatggtga tagtgaccac
      541 agttcccctg ggaacagggc ttcggagagc aaccagccct caccaggctc cacactgtct
      601 tccaggtctg tggaactgaa tggattcatg gccttcaggg aacagtacat gggaatgtct
      661 gtgcctccac attaccaata tataccgcac ctttttagct attctggcca ctcaccactt
      721 ctgccccaac aagctcgcag cctggatccc cagtcataca gtctgattca ccagctgtta
      781 tcagccgagg acctggaacc attgggcacg cccatgttga ttgaagatgg atacgctgtg
      841 acacaggcag aactatttgc cctgctttgc cgcctggccg acgagctgct ctttaggcag
      901 attgcctgga tcaagaaact gcctttcttc tgcgagctct caatcaagga ttacacgtgc
      961 ctcttgagct ctacgtggca ggagctaatc ctgctgtctt ccctcaccgt ttacagcaag
     1021 cagatctttg gggaactggc tgatgtcact gccaagtact cgccctccga tgaagaacta
     1081 cacagattta gtgatgaagg gatggaggtg atcgagcggc tcatctacct ctatcacaag
     1141 ttccatcagc taaaggtcag caacgaggag tatgcttgca tgaaagcaat taacttccta
     1201 aatcaagata tcaggggtct gaccagtgcc tcacagctgg aacaattgaa taaacgatac
     1261 tggtacattt gccaggattt tactgaatat aaatacacac atcagccgaa ccgctttcct
     1321 gatctcatga tgtgcttacc tgagattcga tatattgcag gaaagatggt gaatgtgccc
     1381 ctggagcagc tgcccctcct ctttaaggtg gtgctgcatt cctgcaagac cagtgtgggc
     1441 aaggaatagt aatgaactag tccgtaactt gaaagtattt cgatttcttg gctttatata
     1501 tcttgtggaa aggacgaatg atcagtttcg cacgcaatta t
//
