LOCUS       sequence-284649-        1529 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1529)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1529)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1490
FEATURES             Location/Qualifiers
     source          1..1529
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggagcggga cgaaccgccg cctagcggag ggggaggcgg cgggggctcg
       61 gcggggttcc tggagcctcc cgccgcgctc cctccgccgc cgcgcaacgg tttctgtcag
      121 gatgaattgg cagagcttga cccaggcact aatgatcggg ctgaacaacg aacctgtctc
      181 atttgtgggg accgcgctac aggcttgcac tatgggatca tctcctgtga gggctgcaaa
      241 gggtttttca agcggagcat ttgcaacaaa cgggtatatc gatgcagtcg tgacaagaac
      301 tgtgtcatgt ctcggaagca gaggaacagg tgccagtact gccgcctgct caaatgcctc
      361 cagatgggga tgaaccggaa ggctatcaga gaagatggca tgcctggagg ccggaataag
      421 agcattgggc cagtccagat atcggaagaa gaaatcgaaa ggatcatgtc tgggcaggag
      481 tttgaggaag aggccaatca ctggagcaac catggtgata gtgaccacag ttcccctggg
      541 aacagggctt cggagagcaa ccagccctca ccaggctcca cactgtcttc caggtctgtg
      601 gaactgaatg gattcatggc cttcagggaa cagtacatgg gaatgtctgt gcctccacat
      661 taccaatata taccgcacct ttttagctat tctggccact caccacttct gccccaacaa
      721 gctcgcagcc tggatcccca gtcatacagt ctgattcacc agctgttatc agccgaggac
      781 ctggaaccat tgggcacgcc catgttgatt gaagatggat acgctgtgac acaggcagaa
      841 ctatttgccc tgctttgccg cctggccgac gagctgctct ttaggcagat tgcctggatc
      901 aagaaactgc ctttcttctg cgagctctca atcaaggatt acacgtgcct cttgagctct
      961 acgtggcagg agctaatcct gctgtcttcc ctcaccgttt acagcaagca gatctttggg
     1021 gaactggctg atgtcactgc caagtactcg ccctccgatg aagaactaca cagatttagt
     1081 gatgaaggga tggaggtgat cgagcggctc atctacctct atcacaagtt ccatcagcta
     1141 aaggtcagca acgaggagta tgcttgcatg aaagcaatta acttcctaaa tcaagatatc
     1201 aggggtctga ccagtgcctc acagctggaa caattgaata aacgatactg gtacatttgc
     1261 caggatttta ctgaatataa atacacacat cagccgaacc gctttcctga tctcatgatg
     1321 tgcttacctg agattcgata tattgcagga aagatggtga atgtgcccct ggagcagctg
     1381 cccctcctct ttaaggtggt gctgcattcc tgcaagacca gtgtgggcaa ggaatagtaa
     1441 tgaactagtc cgtaacttga aagtatttcg atttcttggc tttatatatc ttgtggaaag
     1501 gacgaacttc aggttcctta accgacaac
//
