LOCUS       sequence-284667-        1769 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1769)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1769)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1552
FEATURES             Location/Qualifiers
     source          1..1769
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggggagggg cttcaggatg ttcaccatga caagagccat ggaagaggct
       61 ctttttcagc acttcatgca ccagaagctg gggatcgcct atgccataca caagccattt
      121 cccttctttg aaggcctcct agacaactcc atcatcacta agagaatgta catggaatct
      181 ctggaagcct gtagaaattt gatccctgta tccagagtgg tgcacaacat tctcacccaa
      241 ctggagagga cttttaacct gtctcttctg gtgacattgt tcagtcaaat taacctgcgt
      301 gaatatccca atctggtgac gatttacaga agcttcaaac gtgttggtgc ttcctatgaa
      361 tggcagagca gagacacacc aatcctactt gaagccccaa ctggcctagc agaaggaagc
      421 tccctccata ccccactggc gctgccccca ccacaacccc ctcaaccaag ctgttcaccc
      481 tgtgcgccaa gagtcagtga gcctggaaca tcctcccagc aaagcgatga gatcctgagt
      541 gagtcgccca gcccatctga ccctgtcctg cctctccctg cactcatcca ggaaggaaga
      601 agcacttcag tgaccaatga caagttaaca tccaaaatga atgcggaaga agactcagaa
      661 gagatgccca gcctcctcac tagcactgtg caagtggcca gtgacaacct gatcccccaa
      721 ataagagata aagaagaccc tcaagagatg ccccactctc ccttgggctc tatgccagag
      781 ataagagata attctccaga accaaatgac ccagaagagc cccaggaggt gtccagcaca
      841 ccttcagaca agaaaggaaa gaaaagaaaa agatgtatct ggtcaactcc aaaaaggaga
      901 cataagaaaa aaagcctccc aggagggaca gcctcatcta gacacggaat ccaaaagaag
      961 ctcaaaaggg tggatcaggt tcctcaaaag aaagatgact caacttgtaa ctccacggta
     1021 gagacaaggg cccaaaaggc gagaactgaa tgtgcccgaa agtcgagatc agaggagatc
     1081 attgatggca cttcagaaat gaatgaagga aagaggtccc agaagacgcc tagtacacca
     1141 cgaagggtca cacaaggggc agcctcacct gggcatggca tccaagagaa gctccaagtg
     1201 gtggataagg tgactcaaag gaaagacgac tcaacctgga actcagaggt catgatgagg
     1261 gtccaaaagg caagaactaa atgtgcccga aagtccagat tgaaagaaaa gaaaaaggag
     1321 aaagatatct gttcaagctc aaaaaggaga tttcagaaaa atattcaccg aagaggaaaa
     1381 cccaaaagtg acactgtgga ttttcactgt tctaagctcc ccgtgacctg tggtgaggcg
     1441 aaagggattt tatataagaa gaaaatgaaa cacggatcct cagtgaagtg cattcggaat
     1501 gaggatggaa cttggttaac accaaatgaa tttgaagtcg aaggaaaagg aaggaacgca
     1561 aagaactgga aacggaatat acgttgtgaa ggaatgaccc taggagagct gctgaagagt
     1621 ggacttttgc tctgtcctcc aagaataaat ctcaagagag agttaaatag caagtagtaa
     1681 tgaactagtc cgtaacttga aagtatttcg atttcttggc tttatatatc ttgtggaaag
     1741 gacgaggagg gctatatgac atgagtagc
//
