LOCUS       sequence-284676-        2003 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2003)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1601
FEATURES             Location/Qualifiers
     source          1..2003
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggctggtgc tcagactctg ttgacgttca gggatgtggc catagaattc
       61 tccctggagg agtggaaatg cctggacctc gctcagcaga atttgtacag ggatgtgatg
      121 ttggagaact acagaaactt gttctccgtt ggtctcactg tctgtaagcc aggcctgatc
      181 acctgcctgg agcaacgaaa agagccctgg aatgtgaaga gacaggaggc agcagacgga
      241 catccagaga tgggatttca ccatgctact caggcttgtc ttgaactcct gggctcaagc
      301 gatctgcctg cctcagcctc ccaaagtgct gggattacag gtgtgaacca ccgtgcccag
      361 ccaggcctca atgtttctgt ggacaaattc actgcactgt gctcacctgg ggtgctgcaa
      421 actgtgaaat ggtttttaga gttcagatgc atcttctcac tagctatgtc ttctcatttt
      481 acccaagacc ttctgccaga gcagggcata caagatgcat tcccaaaaag aatactgaga
      541 ggatatggaa attgtggcct tgataattta tatttaagga aagactggga aagtttagat
      601 gagtgtaagt tgcaaaaaga ttataatgga cttaaccaat gttcatcaac tacccatagc
      661 aaaatctttc aatataataa atatgttaaa atctttgata acttttcaaa tttacataga
      721 cgtaatataa gtaatactgg agagaaacct ttcaaatgtc aagaatgtgg caaatccttt
      781 caaatgctct cattcctaac tgaacatcag aaaattcaca ctggaaaaaa attccaaaaa
      841 tgtggagaat gtggcaaaac ctttatccag tgctcacact ttactgaacc tgagaacatt
      901 gacactggag agaaacctta caagtgtcaa gaatgtaaca acgtcattaa aacttgctca
      961 gtccttacta aaaatagaat ttatgccgga ggggaacatt acagatgtga agaatttggc
     1021 aaagtattta accagtgctc ccaccttact gaacatgagc atggtactga ggaaaaaccc
     1081 tgcaaatatg aagagtgcag cagtgtcttt atctcttgct caagcctttc taatcaacag
     1141 atgattcttg ctggagagaa gctctccaaa tgtgaaacat ggtacaaagg ttttaaccac
     1201 agcccaaatc cttccaaaca ccagagaaat gagattggag ggaaaccttt caaatgtgag
     1261 gaatgtgaca gcatcttcaa gtggttctca gaccttacta aacataagag aattcacact
     1321 ggtgagaaac catacaaatg tgacgaatgt gggaaagcct atacacagtc ctcacacctc
     1381 agtgaacaca ggaggattca caccggagag aaaccctacc aatgtgaaga atgtgggaag
     1441 gtcttcagaa cttgctcaag cctttctaac cataagagaa ctcattctga agaaaaaccc
     1501 tacacgtgtg aagaatgtgg caacatcttt aagcagttat cagacctcac taagcataag
     1561 aaaacccata ctggagagaa gccctacaaa tgtgacgaat gtggaaaaaa ctttacccag
     1621 tcctccaacc ttattgtaca taagagaatt catactggag agaaacccta caagtgtgaa
     1681 gaatgtggca gagtcttcat gtggttctca gacattacca aacataagaa aacccatact
     1741 ggagagaaac cctacaaatg tgacgaatgt ggaaaaaact ttacccagtc ctcaaacctt
     1801 attgtacata agagaattca tactggagag aaaccctaca agtgtgaaaa gtgtggcaaa
     1861 gccttcaccc agttctcaca cctgactgta catgaaagca ttcatactta gtaatgaact
     1921 agtccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg aaaggacgat
     1981 ctgcaatggg cgtcacgtga ccc
//
