LOCUS       sequence-284679-        1364 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1364)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1614
FEATURES             Location/Qualifiers
     source          1..1364
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcggagct ggggaagaag tactgcgtgt actgcctggc cgaggtgagc
       61 ccgctgcgct tccgctgcac cgagtgccag gacatcgagc tgtgccccga gtgcttctcg
      121 gccggcgccg agatcggcca ccaccgccgc taccacggct accagctggt ggacggcggg
      181 cgcttcacgc tctgggggcc cgaggccgag ggcggctgga ccagtcgcga ggagcagctg
      241 ctgctggacg ccatcgagca gttcggcttc ggaaactggg aagatatggc tgcccacgtt
      301 ggtgcttccc ggactcccca agaggtgatg gagcattacg tgagcatgta catccacggg
      361 aacctgggga aggcctgcat ccccgacacc atccccaacc gcgtgacaga ccacacctgt
      421 cccagcggag gccccctctc acccagcctc accaccccgc tgcccccgct ggacatctct
      481 gtggctgagc agcagcagct gggctacatg ccgctgcggg atgattacga gatcgagtat
      541 gaccaggatg ccgagacgct catcagcggg ctctctgtca actatgatga cgacgacgtg
      601 gagatcgagc tgaagcgcgc ccacgtggac atgtacgtgc ggaagctgaa agagagacag
      661 cggcggaaga acatcgcccg tgactacaat ctggtgccag ccttcctggg gaaggacaag
      721 aaggagaagg aaaaggcgct gaagcgcaag atcaccaagg aggagaagga gctgcgcctg
      781 aagctgaggc cgctgtacca gttcatgtca tgcaaggagt ttgatgacct ttttgaaaac
      841 atgcacaaag aaaaaatgct ccgggccaag atccgagaac tgcagcggta ccggcgaaac
      901 gggatcacca agatggaaga gtcggcagag tacgaggcag cgcggcataa acgggagaag
      961 aggaaggaga acaaaaacct agccggctcc aaacggggaa aggaggacgg caaagacagc
     1021 gagttcgccg ccattgagaa ccttccaggc ttcgagctcc tgtcagatcg cgagaaggtg
     1081 ctctgcagct ctttaaactt gagtccagcc cgctacgtga ctgtgaagac tattataatt
     1141 aaagaccacc tccagaagcg gcaaggaatc ccctccaaaa gccgccttcc tagctacctg
     1201 gacaaagtcc taaagaaaag gattttgaat ttcctcacag aaagcggctg gatctccagg
     1261 gacgcgtctt agtaatgaac tagtccgtaa cttgaaagta tttcgatttc ttggctttat
     1321 atatcttgtg gaaaggacga ccactctaaa tccagatcct cctt
//
