LOCUS       sequence-284691-        8672 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8672)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 8672)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1769
FEATURES             Location/Qualifiers
     source          1..8672
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggaggagaa acagcagatt atattggcta atcaagatgg tggaacagtg
       61 gcaggagcag cacctacctt ctttgtcatc ttaaagcagc caggaaatgg caaaactgat
      121 caaggaattt tggttactaa tcaggatgcc tgtgctttgg ctagtagtgt gtcatcacca
      181 gtaaaatcta aagggaagat ttgccttcca gctgattgta ctgtgggtgg aatcactgtt
      241 accctcgata acaatagtat gtggaatgag ttctatcatc gaagcacaga gatgattctg
      301 accaagcaag gaagacgcat gtttccttac tgtcgttatt ggataacagg tttagattca
      361 aatttgaagt atattcttgt catggatata tctcctgtgg ataaccatcg ttataagtgg
      421 aatggtcgtt ggtgggaacc tagtgggaag gctgaacctc atgttttggg gagggttttc
      481 attcatccag aatctccttc cacaggtcat tattggatgc atcaaccagt atctttctat
      541 aaactcaaac ttaccaacaa tacactggac caagaagggc atatcatctt gcactctatg
      601 catcgttacc tgccgaggct tcatttggtg cctgcagaaa aggctgtgga ggtgatacaa
      661 ttaaatggcc ctggtgtcca cacttttacc ttcccacaga ctgaattctt tgcagtaaca
      721 gcttatcaga acattcagat tactcagctg aaaatagatt acaatccatt tgccaaaggc
      781 tttcgggatg atgggctgaa taataagccc cagagagatg gaaaacaaaa gaacagctct
      841 gaccaagaag ggaataatat ttccagttct tctggtcatc gggtccgtct tacagaaggt
      901 caggggtcag agatacaacc aggtgatttg gatcctttgt caaggggtca tgaaacatca
      961 ggcaagggtt tggagaagac ttcccttaat ataaaacgag actttcttgg tttcatggat
     1021 actgattcag cacttagtga agttcctcaa ttgaagcaag agatttctga atgtcttatt
     1081 gccagcagtt ttgaagatga ctcccgtgta gcctcaccgt tagaccagaa cggaagcttc
     1141 aatgttgtta ttaaagagga acctctagat gattatgact acgaacttgg tgagtgccca
     1201 gaaggggtca ctgtgaaaca ggaagagaca gatgaagaga cggatgtata ctcaaacagt
     1261 gatgatgatc ctatactaga gaaacagcta aagaggcaca ataaagttga caacccagaa
     1321 gctgaccatc tatcttctaa atggcttcca agcagcccat caggtgttgc taaagctaaa
     1381 atgttcaaat tagacactgg aaagatgcca gtagtctatc tggagccctg tgctgtcacc
     1441 agaagcacag ttaagatttc tgaactcccc gataacatgc tttccacatc tcgaaaggat
     1501 aaatcttcta tgttggcaga attggaatat ttgcctacat acattgaaaa ttccaatgag
     1561 actgccttct gcttaggcaa ggaatcagaa aatggtctta gaaaacattc accagatctc
     1621 agagtggtac aaaaatatcc cttactgaaa gagcctcagt ggaaatatcc tgatatatct
     1681 gacagcatta gcacagaaag aatactcgac gattcaaagg attcagttgg agactcactt
     1741 tcaggaaaag aggacttggg cagaaagaga acaactatgc ttaagattgc aacagccgca
     1801 aaggtagtga atgctaatca gaatgcctct ccaaatgtcc ctggaaaaag aggaaggcca
     1861 cgaaaattga aactctgtaa ggcaggacga ccacctaaga acacaggaaa gtctttaatt
     1921 tctacaaaga atacacctgt aagccctggg agtacctttc cagatgtgaa gcctgatctg
     1981 gaagatgtgg atggtgttct ctttgtttcc tttgaatcaa aggaagctct agacattcat
     2041 gcagttgatg ggacaacaga agaatcttct agtctccagg catcaaccac aaatgactca
     2101 ggttacagag caagaatttc ccagttggaa aaggaattga tagaagattt gaagactttg
     2161 cggcacaagc aggtgataca tcctggtctt caagaagtgg gcttaaaatt gaattcagtg
     2221 gatccaacaa tgagcattga tcttaaatac ttgggagtac agttaccttt ggctccagct
     2281 actagctttc ctttttggaa ccttacagga accaaccctg cctctcctga tgcgggattt
     2341 ccctttgttt ctaggacagg gaaaaccaat gatttcacta agatcaaggg atggagggga
     2401 aaatttcata gtgcttctgc atctaggaat gaaggtggaa attcagaaag ttcactgaaa
     2461 aatcgttctg ctttctgtag tgataagcta gatgaatact tggaaaatga aggcaagctg
     2521 atggaaacaa gcatgggttt ttcttctaat gctcccacat ctcctgtggt gtaccagctt
     2581 cccactaaga gtaccagtta tgtacgaaca cttgatagtg tactaaagaa gcaatctact
     2641 atttcccctt ctacctctta ttctttgaaa cctcattctg taccccctgt ctctcgaaag
     2701 gcaaagtctc aaaacagaca ggcaactttc agtggccgaa ctaaatcatc ttataaatcc
     2761 attttaccat accctgtttc accaaagcag aaatactctc atgtgattct aggagataag
     2821 gttaccaaga attcttcagg catcatctca gaaaatcagg cgaataactt tgttgtgcca
     2881 actttggatg aaaatatatt tccaaagcag attagtttgc ggcaggcaca gcagcagcag
     2941 caacagcaac agggaagtcg ccctccaggc ttgtctaaat ctcaggtgaa gctaatggac
     3001 ctggaagact gtgcactttg ggaaggaaaa ccaaggacat acatcacaga agagcgagca
     3061 gatgtatcct taacaactct acttacagct caagcatccc tcaaaactaa acctatccac
     3121 acaatcataa ggaaacgagc ccctccctgc aacaatgact tctgtcgact gggttgtgta
     3181 tgttccagtc tagctttgga gaagcgccaa cctgctcact gccgccgacc agactgcatg
     3241 tttggttgta cttgtttgaa aagaaaagtt gtacttgtta aaggaggatc caaaactaag
     3301 cattttcaga ggaaggctgc tcatcgagat ccagtatttt atgatactct gggagaggag
     3361 gcaagggagg aggaagaagg aatcagggag gaggaggaac aattgaaaga gaaaaagaag
     3421 agaaagaagc tagaatacac tatatgtgag acagagcctg aacagcctgt tcgacattac
     3481 ccattatggg taaaagtaga aggtgaagta gatccagaac cagtttatat ccccacgcct
     3541 tctgtcattg agcctatgaa accattgtta ttgcctcagc cagaagtttt atctcctact
     3601 gtgaagggca aactgctcac tggaattaaa tctccacggt catatactcc caaacccaat
     3661 cctgtgattc gggaagagga caaagatcca gtctacttgt actttgaaag tatgatgact
     3721 tgtgctcgag ttcgagtata tgagcgaaaa aaagaggacc agagacaacc atcttcctcc
     3781 tcctccccat ctccatcatt tcagcagcaa acttcatgtc attctagccc tgagaaccat
     3841 aataatgcaa aggaacctga ttctgaacag cagcccttaa aacaactcac ctgtgacttg
     3901 gaggatgatt ctgataaatt acaagaaaag agctggaagt cttcctgcaa tgaaggagaa
     3961 tcctcttcta cttcttatat gcatcagagg tcacctggtg gtcccaccaa actgattgag
     4021 atcatctcag actgcaactg ggaggaagat cggaacaaga ttttgagcat cttatcccag
     4081 cacatcaata gcaacatgcc acaatcactt aaggtgggca gcttcatcat tgagttggct
     4141 tctcagcgaa agagccgggg tgagaagaac cctcctgttt attcttctcg tgtgaaaatc
     4201 tctatgccat catgtcaaga ccaagatgat atggctgaga aatctggatc agagactcct
     4261 gatggtccat tgtcccctgg gaaaatggag gatatctctc ctgtgcagac agatgccctg
     4321 gattcagtga gggagagatt acatggaggc aaaggtctgc ctttttatgc agggctttct
     4381 cctgcaggga agcttgtggc ctataaacgt aaacccagtt caagtacatc tgggcttatc
     4441 caggtagcat ccaatgccaa ggtggctgca tccaggaaac cacgtaccct gttgccttca
     4501 acatccaatt ccaaaatggc atcctcctct ggcactgcaa caaatcgccc tgggaagaat
     4561 ctgaaggcgt ttgtcccagc aaaacggcca attgaaaatg ctgctcaaat tccagtggct
     4621 actccacagg tctctcctaa cacagtgaaa cgtgctggac ctcgattgtt gttgattcca
     4681 gtgcagcagg gttctcctac tcttagacct gtctcaaaca cacaacttca gggacatcgg
     4741 atggtcttgc agcctgttag gagtccaagt ggaatgaact tattcaggca ccctaatggg
     4801 cagattgtcc agcttctacc tttgcatcag cttcgaggct ctaataccca gcccaactta
     4861 cagcctgtca tgtttcggaa cccagggtct gtgatgggaa tccggttacc tgctccttcc
     4921 aaaccctctg agactccgcc atcttccact tcgtcctctg ctttctctgt catgaatcct
     4981 gtaattcaag ctgttgggtc ttcttcagca gtgaatgtta tcactcaggc accatcattg
     5041 ctttcctctg gagctagttt tgtgtctcag gctggtacat tgaccctgag gatttctcct
     5101 cctgaaccac aaagctttgc aagtaaaaca ggctctgaaa ccaaaataac ttatagctca
     5161 ggaggacagc ctgttggtac agccagtctt attcctctcc agtctggtag ttttgccttg
     5221 ttacagctcc caggacaaaa gcctgttcct agctccattc ttcagcatgt tgcttccctt
     5281 cagatgaaga gagaatctca gaatccagac cagaaagatg aaacaaactc aataaaaaga
     5341 gagcaagaaa cgaagaaggt tctacagtca gaaggagagg ctgtagaccc tgaggctaat
     5401 gtaataaaac aaaactcagg agctgctacc tcagaagaaa ctctgaatga ttccttggaa
     5461 gataggggtg atcatttgga tgaagaatgc cttccagaag aaggttgtgc aactgtcaaa
     5521 ccatctgagc attcctgtat cactgggtca catacagatc aagattataa agatgttaat
     5581 gaagaatatg gggctaggaa tcgtaagagt tccaaagaaa aagtggctgt tctggaagtt
     5641 aggaccattt ctgaaaaagc cagtaataag acagtccaaa atttaagtaa agtacagcat
     5701 caaaaacttg gtgatgtgaa ggtggaacag cagaaaggat ttgacaatcc agaagaaaac
     5761 tcaagtgaat ttccagtcac ctttaaggaa gaaagtaaat ttgaattgtc aggaagcaaa
     5821 gttatggagc agcaatctaa tctacagcca gaggccaaag agaaggaatg tggagactct
     5881 ctggagaaag acagggaaag atggagaaaa catctgaagg gccccttaac caggaaatgt
     5941 gttggagctt cacaggaatg taagaaagag gcagacgagc agttaattaa agaaacaaag
     6001 acatgtcagg aaaattcaga tgtgtttcag caagaacaag gcatctctga cttacttgga
     6061 aaaagtggaa ttactgaaga tgccagagtt ttgaaaactg aatgtgattc ttggagtagg
     6121 atttctaatc cttcagcctt ctccattgtt cctaggagag ctgcaaaaag cagcagaggg
     6181 aatggacatt ttcagggtca cttactgcta cctggagaac agatacaacc aaagcaagag
     6241 aagaagggtg ggagaagcag tgctgacttc actgttttgg atttggaaga agatgatgaa
     6301 gatgataatg agaaaactga tgattctatt gatgaaattg tggatgttgt ttctgactac
     6361 cagagtgagg aggttgatga tgtagaaaag aataactgtg tagaatacat tgaggatgat
     6421 gaggagcacg tggacattga gactgtagaa gagctctcag aggaaattaa tgttgctcac
     6481 ctgaagacca cagcggccca cacacagtca ttcaaacagc cgtcctgtac tcacatctct
     6541 gcagatgaaa aagcagctga aaggagtcga aaggctccac caattcctct aaaactgaag
     6601 cctgattact ggagtgacaa actacagaaa gaagcagaag cgtttgctta ttatcgccgg
     6661 acacacactg ccaatgagcg gcggcggcgt ggtgaaatga gggatctctt tgagaaatta
     6721 aagatcacat tgggattact tcattcttcc aaggtttcca aaagtctcat tcttactcga
     6781 gccttcagtg aaattcaggg actaacagat caggcagaca aattgatagg acagaaaaat
     6841 ctcctgactc gaaaacggaa tattctgata cggaaagtat cgtctctttc aggtaagaca
     6901 gaagaagtgg tcctgaagaa gctagagtat atttatgcaa aacagcaagc actagaggca
     6961 caaaaaagaa aaaagaagat gggatcagat gagtttgaca tatctcccag aattagcaaa
     7021 cagcaggaag gatcttctgc atcatctgta gatcttggac agatgtttat aaataacagg
     7081 agggggaaac ctttgattct ttccagaaaa aaagaccagg ccacagaaaa tacctcaccc
     7141 ttgaacactc cacacacctc tgccaacctt gtgatgactc cgcaagggca attgctcacc
     7201 ctaaaaggtc ccctattctc aggaccagtg gtagctgttt ctcctgatct cttagaatct
     7261 gatcttaagc ctcaagttgc cggtagtgct gtggctctac cagaaaatga cgacttattt
     7321 atgatgccac gaattgttaa tgtgacatca ttggccacag agggaggttt ggtagatatg
     7381 ggtggcagca aatatcctca tgaagttcct gatagcaagc catctgacca tctgaaagac
     7441 accgtcagga atgaagataa ttccttagag gataagggta gaatctcttc cagaggaaac
     7501 agagatggca gagtgacgtt gggtccaacg caggtttttc tggcaaacaa agattctggt
     7561 tatccacaaa tagttgacgt ttccaatatg cagaaagcac aagagttctt acctaaaaag
     7621 atttctggtg atatgagagg gattcagtat aaatggaaag agagtgaatc aagaggggag
     7681 agagtgaagt caaaggattc ttcatttcat aaattaaaga tgaaagatct caaggactca
     7741 agcatagaga tggaactgag gaaagtaaca tcagctatag aggaagcagc tcttgattcc
     7801 agtgaactgc tgactaacat ggaagatgag gatgatactg atgagacact gacttcactg
     7861 ctcaatgaaa ttgcctttct taatcaacaa ctaaatgatg actctgttgg cctggctgaa
     7921 ctacccagct ctatggatac agagttccca ggggatgctc ggcgggcttt tattagtaag
     7981 gttcctcctg gaagcagagc aactttccag gttgagcact tgggaactgg tttgaaagag
     8041 ttgcctgatg ttcaagggga gagtgactct atcagtcccc tcctcttgca cttggaagac
     8101 gatgactttt ctgagaatga aaaacaactt gcagaaccag cctctgagcc agatgtcctt
     8161 aagattgtta ttgactctga aataaaggat tccctccttt ccaacaagaa agctattgat
     8221 ggagggaaga atacttctgg cctccctgca gagcccgaaa gtgtgtcctc accccccacc
     8281 ctacacatga agactggctt ggagaacagc aacagcacag acactttgtg gaggcctatg
     8341 ccaaagttgg cccctctagg tttaaaagta gctaatcctt ccagtgatgc agatggtcag
     8401 agtctcaagg tgatgccttg tttggcacct atagctgcca aagttgggtc agttggacac
     8461 aaaatgaact taacagggaa tgaccaggaa ggccgggaaa gcaaggtgat gcctacattg
     8521 gcacctgttg tggctaaatt gggcaactcg ggggcctcac caagttctgc agggaaatag
     8581 taatgaacta gtccgtaact tgaaagtatt tcgatttctt ggctttatat atcttgtgga
     8641 aaggacgaac gccgttaata gcaggattta ca
//
