LOCUS       sequence-284693-       15103 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15103)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 15103)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1802
FEATURES             Location/Qualifiers
     source          1..15103
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    10960..11548
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(11013..11033)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(11431..11442)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             11620..11853
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    11931..12052
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11968..11987)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12022..12041
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      12092..12227
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(12247..12266)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(12248..12266)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(12276..12293)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(12276..12292)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12285..12307)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      12434..12889
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(12521..12540)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     12731..12752
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        12915..13019
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(13020..13088,13089..13880)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(13238..13257)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      14051..14639
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     14540..14559
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     14793..14810
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    14927..14948
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(14963..14968,14969..14986,14987..14993)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    15001..15017
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     15006..15028
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     15025..15041
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     15025..15041
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     15060..15080
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        15062..15080
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatgcca gaaatgacag agaatgagac
     4141 gcctacaaaa aagcagcaca gaaagaaaaa ccgggagaca cacaatgcag tggagaggca
     4201 tagaaagaag aaaattaatg ctgggataaa cagaatagga gagctgatcc catgttctcc
     4261 tgccctgaag cagagcaaga atatgatcct ggaccaagcc tttaaatata taacagaatt
     4321 gaaaaggcaa aatgatgaac tcctgcttaa tggaggaaac aatgaacaag ctgaagaaat
     4381 aaaaaagcta cggaaacaac tggaagaaat ccaaaaagaa aatggccgat atattgaatt
     4441 actgaaagct aatgacatat gcttatatga tgacccgaca attcactgga aaggaaatct
     4501 taaaaactca aaggtctctg ttgttattcc tagtgaccag gttcaaaaaa aaattattgt
     4561 ttattccaac gggaatcagc ctggtggaaa cagccaggga acagctgttc aggggataac
     4621 ttttaatgtt agtcataatt tacaaaagca gaccgccaat gtggtgccag tacagaggac
     4681 ttgcaatctt gtgactcctg tatctatttc tggagtttac ccttctgaaa acaagccatg
     4741 gcatcagacc acagttcctg cattggctac caaccagcct gttcctcttt gtcttcctgc
     4801 tgccatttct gctcagagta ttctcgagct tcccacctct gaaagcgaat caaatgtgct
     4861 tggtgccact agtggctcac tgattgctgt ttcaattgaa tctgagcctc accaacatca
     4921 ttctttgcac acatgcctaa atgatcaaaa ttcttctgaa aataagaatg gacaagagaa
     4981 ccccaaagta ttgaagaaaa tgaccccttg tgttacaaac atcccccaca gctcctcagc
     5041 aactgccact aaagtgcacc atggaaacaa gtcctgcctg agcatacagg acttcagagg
     5101 tgattttcaa aacacttttg ttgtttcagt taccaccaca gtctgctcgc agcctcccag
     5161 aactgcaggt gattcttctc caatgagcat tagtaagagt gcagacttga caagtacagc
     5221 tacagtggtg gcatcatctg cccctggagt agggaaggcc accattccta taagcactct
     5281 ttcgggaaac cctttggaca atggttggac tctttcttgt tctttgcctt cttcaagtgt
     5341 tagtacttca gatttgaaaa acattaatag ccttacacga atctcttcag ctggaaacac
     5401 acagacaacg tggactactt tgcaactggc aggaaacact attcagccct taagccagac
     5461 accatcttct gctgtgactc cagtattaaa tgagtctggt actagcccca ccacaagcaa
     5521 ccacagtaga tatgtggcta cagacatcaa cttgaataat tcctttccag cagatgggca
     5581 gccagttgag caagtagttg taacattgcc ttcttgtcca tctttaccta tgcagccact
     5641 aattgcccag ccacaagtta aatctcagcc tcccaaaaat atccttccac tgaattcagc
     5701 aatgcaagtg attcagatgg ctcagccagt tgggtcagct gttaattcag ctccaactaa
     5761 tcaaaatgtt ataattcttc agccacctag caccacccca tgcccaacag tgatgagggc
     5821 agaagtttcc aaccaaacag taggtcaaca gatagtaatc atacaggctg ctaatcagaa
     5881 tcctttgcca ctcctccctg ctccacctcc tggttctgtt cgactcccca tcaatggagc
     5941 caatactgta atagggtcta ataattcagt gcaaaatgtt ccaacaccac agacttttgg
     6001 aggaaagcat cttgtccaca tattaccaag accttcatct ttatcagcgt ctaactcaac
     6061 acaaactttt tctgttacca tgtcaaacca acagcctcaa accatttctt taaatggaca
     6121 gctctttgct ttgcagcctg tgatgtcttc atcaggaact acaaatcaaa cccctatgca
     6181 aattattcaa cccaccacca gcgaagatcc aaataccaat gttgccctga atacatttgg
     6241 tgctttggcc agcctcaatc aaagcatatc acagatggct gggcaaagct gtgtacaatt
     6301 gtctattagc cagcctgcca attctcaaac tgctgcaaat agtcaaacca ctacagctaa
     6361 ctgtgtttca ttaacaacaa ctgcagcacc tcctgtgaca acagatagtt cagccacact
     6421 agctagtact tataatctag tgagtacttc ctcaatgaac actgttgctt gtttgcctaa
     6481 catgaaatct aaaaggttga ataagaagcc aggtggcagg aaacacttag cagcaaacaa
     6541 gtcagcgtgt cccctgaatt cagtcagaga tgtgagcaag ttagactgcc ccaacactga
     6601 aggctcagca gagccaccct gtaatgatgg actgctagaa agcttccctg ctgtgttacc
     6661 atctgtctct gtgtctcagg caaatagtgt gagtgtttct gcttcacatt ctttgggtgt
     6721 tctaagctct gaatcattaa tacctgagtc tgtatcgaaa tctaagtcag cagaaaagtc
     6781 cagcccaccc tcccaagaat ctgtaacaag tgaacatttt gcaatggccg cagcaaaatc
     6841 caaagattct acccctaatc tacaacaaga gacatctcag gataaaccac caagtagttt
     6901 agcattatca gatgctgcca aaccctgcgc ttcagccaat gtattgattc catctccaag
     6961 tgatcctcac attttggttt ctcaggttcc tggtttgtca tctacaacaa gcactacaag
     7021 tactgactgt gtttctgagg tagaaatcat tgctgaacct tgcagagttg agcaagattc
     7081 atcagataca atgcaaacca caggtctctt aaaggggcaa ggtttaacta cattgctatc
     7141 tgatcttgct aaaaaaaaaa accctcagaa atcatctctt tctgatcaga tggatcatcc
     7201 tgacttttct tcagaaaatc ctaaaatagt tgattcaagt gtgaatttac atcccaaaca
     7261 ggaactatta ctgatgaaca atgatgatag agatcctcca cagcatcatt cctgcctccc
     7321 tgatcaagag gttattaatg gttctttgat caacggtaga caggccgact ctcccatgtc
     7381 aaccagctct ggcagtagtc gtagtttctc agttgcatcc atgcttcctg aaacaacaag
     7441 agaagatgtg accagcaatg caacaactaa tacatgtgac agctgtacct ttgtagagca
     7501 aactgatata gtagctcttg cagcaagagc tatttttgac caggagaacc ttgagaaggg
     7561 gagagttggc ctccaggctg atataaggga agttgcttca aagccttctg aagcatcatt
     7621 gttagaggga gacccaccct tcaaatcaca gatacctaaa gagagtggca caggacaggc
     7681 agaagcaaca ccaaatgaat ttaattctca gggttcaatt gaagcaacta tggagaggcc
     7741 ccttgagaaa ccaagttgtt ctctaggaat taaaacatca aatgcatctt tacaggattc
     7801 aacttctcag ccaccaagca tcaccagttt aagtgtgaat aatcttatcc atcagagcag
     7861 catcagccat cctctggcca gctgtgcggg tttatcccca acttcagagc aaacaactgt
     7921 gcctgcaacg gttaatctga ccgtttcatc tagctcctat ggcagtcaac ctcctggacc
     7981 atctctgatg actgaatatt cccaagaaca gctaaatact atgactagta ccataccaaa
     8041 ttcacagatt caagagccac tcttaaagcc aagccatgaa agccgtaagg attctgctaa
     8101 gcgtgctgtc caagatgacc ttttactgtc ttcagctaaa cgtcaaaagc actgtcagcc
     8161 agcccccctc aggcttgaaa gtatgtccct gatgagcaga actccagaca ccatttctga
     8221 ccaaactcaa atgatggtca gtcagatccc tcctaattct tcaaactcag ttgtgcctgt
     8281 tagcaaccca gctcatggag atggccttac acgattattt ccacctagta acaactttgt
     8341 gactcctgca ttgaggcaaa ctgaagttca gtgtggttct cagccttcag ttgctgaaca
     8401 gcagcaaacc caggcaagtc agcatctaca ggccctgcag cagcatgttc cagctcaagg
     8461 tgtatctcac cttcatagta accatctcta cataaagcag cagcagcagc agcagcagca
     8521 gcagcagcag caacagcaac aacaacaagc agggcagtta agagagaggc atcacttata
     8581 tcaaatgcag catcatgtac ctcatgcaga gagctctgtc cactctcagc cccataatgt
     8641 ccaccaacag aggactctgc aacaggaagt tcagatgcag aaaaagagga atcttgttca
     8701 gggcacccag acctctcagc tttccttaca accaaagcac catggaactg accaatcccg
     8761 atccaagact ggccagccac atccccacca tcagcagatg cagcaacaaa tgcagcaaca
     8821 ctttggaagc tcccagacag agaagagctg tgaaaaccct tcaactagtc ggaaccatca
     8881 taaccatccc cagaaccatc tcaatcaaga tattatgcac caacagcagg atgttggaag
     8941 cagacagcaa ggttcagggg tttcatctga acatgtatct ggccataatc caatgcagag
     9001 gcttttgaca tcaagaggct tagagcagca aatggtgtcc caaccaagta ttgtgactag
     9061 atcttcagac atgacctgta ctccacacag gccagagaga aatagagttt caagttattc
     9121 tgctgaggca ctcattggaa agacatcttc taattcagag cagagaatgg ggatatcaat
     9181 tcagggttcc agagtttcag atcagcttga aatgagaagc tatcttgatg ttcccagaaa
     9241 taagagtttg gctattcata atatgcaggg tcgtgtggac catactgtgg cctcagatat
     9301 ccgcctttct gattgtcaga cgtttaaacc aagtggagct agtcaacagc cccagagtaa
     9361 ttttgaagta caatcttcaa gaaacaatga aataggtaac cctgtatcat cattgcggag
     9421 tatgcagtcc caggcttttc gaattagtca aaacactggc cccccaccaa ttgaccgtca
     9481 aaagagatta tcttacccac cagttcagag catcccaaca ggaaatggta ttccatcaag
     9541 ggacagtgaa aatacttgtc accaaagttt catgcagagc ttacttgccc ctcacctcag
     9601 tgatcaggtc attgggagcc agaggtcact ctcagaacat cagaggaata cacagtgtgg
     9661 tccatcctct gcaattgaat ataattgtcc cccaactcat gaaaatgtcc atattagaag
     9721 agagagtgag agtcagaata gggaaagttg tgacatgtcg ttaggtgcaa ttaacaccag
     9781 gaacagcacc ttgaatattc ctttttcaag ttcctcttcc tcaggagata ttcaaggtcg
     9841 aaacacaagc cccaatgttt ctgtacagaa atccaatccc atgaggatta ctgagagtca
     9901 tgccaccaag ggccacatga accctccagt cacaaccaac atgcatgggg ttgcaaggcc
     9961 agcgttgcca catccatctg tgtctcatgg aaatggcgat caaggccctg ctgtacgtca
    10021 agctaattct tcagttcccc agagatcaag gcatcccctg caagacagca gtggttccaa
    10081 aattcgtcag cctgaaagga atcgttctgg aaaccaaagg caaagtactg tctttgatcc
    10141 aagtcttccc catcttcctc tctctactgg tggcagtatg attcttggac gtcaacaacc
    10201 tgccacagag aagagaggaa gtattgttcg tttcatgcct gatagcccac aagtacctaa
    10261 tgataattca ggtcctgacc agcatacact atcacaaaat tttggttttt cttttattcc
    10321 tgagggtggc atgaatccac caataaatgc taatgcttct ttcattccac aggttactca
    10381 gcctagtgcc actcgaactc cagccctcat cccggtagat ccgcaaaata ctctgccctc
    10441 cttctatcct ccatactctc ctgctcatcc tacactgtcc aatgatattt caatccccta
    10501 ttttcctaat cagatgttct caaatcctag cacagagaag gtaaacagtg gaagtttaaa
    10561 taaccgattt ggatcaattt tatctcctcc cagacctgtt gggtttgctc aaccaagttt
    10621 tcctcttctc ccagatatgc caccaatgca catgaccaac tctcacttat ccaattttaa
    10681 tatgacatct ttgtttccag aaatagccac agcgcttcct gatggctcag caatgtcacc
    10741 tttgcttaca atagcaaatt cctctgcctc tgactcttcc aagcagtcct caaacagacc
    10801 tgcccataac ataagccata ttctaggtca tgattgcagt tcagctgttt agtaatgaac
    10861 tagtccgtaa cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga
    10921 caaccttaga cctaaggaac ggaaacgcgt taagtcgaca atcaacctct ggattacaaa
    10981 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
    11041 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
    11101 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
    11161 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
    11221 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
    11281 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
    11341 gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt
    11401 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
    11461 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
    11521 cggatctccc tttgggccgc ctccccgcgt cgactttaag accaatgact tacaaggcag
    11581 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc
    11641 aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag
    11701 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
    11761 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
    11821 gaccctttta gtcagtgtgg aaaatctcta gcagtacgta tagtagttca tgtcatctta
    11881 ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta
    11941 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
    12001 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
    12061 ggctctagct atcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
    12121 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
    12181 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag ggacgtaccc
    12241 aattcgccct atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt
    12301 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc
    12361 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
    12421 aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
    12481 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
    12541 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
    12601 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
    12661 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
    12721 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
    12781 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
    12841 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt
    12901 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
    12961 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
    13021 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
    13081 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
    13141 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
    13201 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
    13261 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
    13321 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
    13381 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
    13441 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
    13501 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
    13561 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
    13621 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
    13681 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
    13741 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
    13801 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
    13861 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
    13921 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
    13981 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
    14041 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
    14101 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
    14161 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
    14221 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
    14281 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
    14341 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
    14401 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
    14461 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
    14521 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
    14581 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
    14641 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
    14701 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
    14761 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
    14821 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
    14881 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
    14941 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
    15001 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg
    15061 caattaaccc tcactaaagg gaacaaaagc tggagctgca agc
//
