LOCUS       sequence-284694-        6839 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6839)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 6839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1802
FEATURES             Location/Qualifiers
     source          1..6839
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 accgccacca tgccagaaat gacagagaat gagacgccta caaaaaagca gcacagaaag
       61 aaaaaccggg agacacacaa tgcagtggag aggcatagaa agaagaaaat taatgctggg
      121 ataaacagaa taggagagct gatcccatgt tctcctgccc tgaagcagag caagaatatg
      181 atcctggacc aagcctttaa atatataaca gaattgaaaa ggcaaaatga tgaactcctg
      241 cttaatggag gaaacaatga acaagctgaa gaaataaaaa agctacggaa acaactggaa
      301 gaaatccaaa aagaaaatgg ccgatatatt gaattactga aagctaatga catatgctta
      361 tatgatgacc cgacaattca ctggaaagga aatcttaaaa actcaaaggt ctctgttgtt
      421 attcctagtg accaggttca aaaaaaaatt attgtttatt ccaacgggaa tcagcctggt
      481 ggaaacagcc agggaacagc tgttcagggg ataactttta atgttagtca taatttacaa
      541 aagcagaccg ccaatgtggt gccagtacag aggacttgca atcttgtgac tcctgtatct
      601 atttctggag tttacccttc tgaaaacaag ccatggcatc agaccacagt tcctgcattg
      661 gctaccaacc agcctgttcc tctttgtctt cctgctgcca tttctgctca gagtattctc
      721 gagcttccca cctctgaaag cgaatcaaat gtgcttggtg ccactagtgg ctcactgatt
      781 gctgtttcaa ttgaatctga gcctcaccaa catcattctt tgcacacatg cctaaatgat
      841 caaaattctt ctgaaaataa gaatggacaa gagaacccca aagtattgaa gaaaatgacc
      901 ccttgtgtta caaacatccc ccacagctcc tcagcaactg ccactaaagt gcaccatgga
      961 aacaagtcct gcctgagcat acaggacttc agaggtgatt ttcaaaacac ttttgttgtt
     1021 tcagttacca ccacagtctg ctcgcagcct cccagaactg caggtgattc ttctccaatg
     1081 agcattagta agagtgcaga cttgacaagt acagctacag tggtggcatc atctgcccct
     1141 ggagtaggga aggccaccat tcctataagc actctttcgg gaaacccttt ggacaatggt
     1201 tggactcttt cttgttcttt gccttcttca agtgttagta cttcagattt gaaaaacatt
     1261 aatagcctta cacgaatctc ttcagctgga aacacacaga caacgtggac tactttgcaa
     1321 ctggcaggaa acactattca gcccttaagc cagacaccat cttctgctgt gactccagta
     1381 ttaaatgagt ctggtactag ccccaccaca agcaaccaca gtagatatgt ggctacagac
     1441 atcaacttga ataattcctt tccagcagat gggcagccag ttgagcaagt agttgtaaca
     1501 ttgccttctt gtccatcttt acctatgcag ccactaattg cccagccaca agttaaatct
     1561 cagcctccca aaaatatcct tccactgaat tcagcaatgc aagtgattca gatggctcag
     1621 ccagttgggt cagctgttaa ttcagctcca actaatcaaa atgttataat tcttcagcca
     1681 cctagcacca ccccatgccc aacagtgatg agggcagaag tttccaacca aacagtaggt
     1741 caacagatag taatcataca ggctgctaat cagaatcctt tgccactcct ccctgctcca
     1801 cctcctggtt ctgttcgact ccccatcaat ggagccaata ctgtaatagg gtctaataat
     1861 tcagtgcaaa atgttccaac accacagact tttggaggaa agcatcttgt ccacatatta
     1921 ccaagacctt catctttatc agcgtctaac tcaacacaaa ctttttctgt taccatgtca
     1981 aaccaacagc ctcaaaccat ttctttaaat ggacagctct ttgctttgca gcctgtgatg
     2041 tcttcatcag gaactacaaa tcaaacccct atgcaaatta ttcaacccac caccagcgaa
     2101 gatccaaata ccaatgttgc cctgaataca tttggtgctt tggccagcct caatcaaagc
     2161 atatcacaga tggctgggca aagctgtgta caattgtcta ttagccagcc tgccaattct
     2221 caaactgctg caaatagtca aaccactaca gctaactgtg tttcattaac aacaactgca
     2281 gcacctcctg tgacaacaga tagttcagcc acactagcta gtacttataa tctagtgagt
     2341 acttcctcaa tgaacactgt tgcttgtttg cctaacatga aatctaaaag gttgaataag
     2401 aagccaggtg gcaggaaaca cttagcagca aacaagtcag cgtgtcccct gaattcagtc
     2461 agagatgtga gcaagttaga ctgccccaac actgaaggct cagcagagcc accctgtaat
     2521 gatggactgc tagaaagctt ccctgctgtg ttaccatctg tctctgtgtc tcaggcaaat
     2581 agtgtgagtg tttctgcttc acattctttg ggtgttctaa gctctgaatc attaatacct
     2641 gagtctgtat cgaaatctaa gtcagcagaa aagtccagcc caccctccca agaatctgta
     2701 acaagtgaac attttgcaat ggccgcagca aaatccaaag attctacccc taatctacaa
     2761 caagagacat ctcaggataa accaccaagt agtttagcat tatcagatgc tgccaaaccc
     2821 tgcgcttcag ccaatgtatt gattccatct ccaagtgatc ctcacatttt ggtttctcag
     2881 gttcctggtt tgtcatctac aacaagcact acaagtactg actgtgtttc tgaggtagaa
     2941 atcattgctg aaccttgcag agttgagcaa gattcatcag atacaatgca aaccacaggt
     3001 ctcttaaagg ggcaaggttt aactacattg ctatctgatc ttgctaaaaa aaaaaaccct
     3061 cagaaatcat ctctttctga tcagatggat catcctgact tttcttcaga aaatcctaaa
     3121 atagttgatt caagtgtgaa tttacatccc aaacaggaac tattactgat gaacaatgat
     3181 gatagagatc ctccacagca tcattcctgc ctccctgatc aagaggttat taatggttct
     3241 ttgatcaacg gtagacaggc cgactctccc atgtcaacca gctctggcag tagtcgtagt
     3301 ttctcagttg catccatgct tcctgaaaca acaagagaag atgtgaccag caatgcaaca
     3361 actaatacat gtgacagctg tacctttgta gagcaaactg atatagtagc tcttgcagca
     3421 agagctattt ttgaccagga gaaccttgag aaggggagag ttggcctcca ggctgatata
     3481 agggaagttg cttcaaagcc ttctgaagca tcattgttag agggagaccc acccttcaaa
     3541 tcacagatac ctaaagagag tggcacagga caggcagaag caacaccaaa tgaatttaat
     3601 tctcagggtt caattgaagc aactatggag aggccccttg agaaaccaag ttgttctcta
     3661 ggaattaaaa catcaaatgc atctttacag gattcaactt ctcagccacc aagcatcacc
     3721 agtttaagtg tgaataatct tatccatcag agcagcatca gccatcctct ggccagctgt
     3781 gcgggtttat ccccaacttc agagcaaaca actgtgcctg caacggttaa tctgaccgtt
     3841 tcatctagct cctatggcag tcaacctcct ggaccatctc tgatgactga atattcccaa
     3901 gaacagctaa atactatgac tagtaccata ccaaattcac agattcaaga gccactctta
     3961 aagccaagcc atgaaagccg taaggattct gctaagcgtg ctgtccaaga tgacctttta
     4021 ctgtcttcag ctaaacgtca aaagcactgt cagccagccc ccctcaggct tgaaagtatg
     4081 tccctgatga gcagaactcc agacaccatt tctgaccaaa ctcaaatgat ggtcagtcag
     4141 atccctccta attcttcaaa ctcagttgtg cctgttagca acccagctca tggagatggc
     4201 cttacacgat tatttccacc tagtaacaac tttgtgactc ctgcattgag gcaaactgaa
     4261 gttcagtgtg gttctcagcc ttcagttgct gaacagcagc aaacccaggc aagtcagcat
     4321 ctacaggccc tgcagcagca tgttccagct caaggtgtat ctcaccttca tagtaaccat
     4381 ctctacataa agcagcagca gcagcagcag cagcagcagc agcagcaaca gcaacaacaa
     4441 caagcagggc agttaagaga gaggcatcac ttatatcaaa tgcagcatca tgtacctcat
     4501 gcagagagct ctgtccactc tcagccccat aatgtccacc aacagaggac tctgcaacag
     4561 gaagttcaga tgcagaaaaa gaggaatctt gttcagggca cccagacctc tcagctttcc
     4621 ttacaaccaa agcaccatgg aactgaccaa tcccgatcca agactggcca gccacatccc
     4681 caccatcagc agatgcagca acaaatgcag caacactttg gaagctccca gacagagaag
     4741 agctgtgaaa acccttcaac tagtcggaac catcataacc atccccagaa ccatctcaat
     4801 caagatatta tgcaccaaca gcaggatgtt ggaagcagac agcaaggttc aggggtttca
     4861 tctgaacatg tatctggcca taatccaatg cagaggcttt tgacatcaag aggcttagag
     4921 cagcaaatgg tgtcccaacc aagtattgtg actagatctt cagacatgac ctgtactcca
     4981 cacaggccag agagaaatag agtttcaagt tattctgctg aggcactcat tggaaagaca
     5041 tcttctaatt cagagcagag aatggggata tcaattcagg gttccagagt ttcagatcag
     5101 cttgaaatga gaagctatct tgatgttccc agaaataaga gtttggctat tcataatatg
     5161 cagggtcgtg tggaccatac tgtggcctca gatatccgcc tttctgattg tcagacgttt
     5221 aaaccaagtg gagctagtca acagccccag agtaattttg aagtacaatc ttcaagaaac
     5281 aatgaaatag gtaaccctgt atcatcattg cggagtatgc agtcccaggc ttttcgaatt
     5341 agtcaaaaca ctggcccccc accaattgac cgtcaaaaga gattatctta cccaccagtt
     5401 cagagcatcc caacaggaaa tggtattcca tcaagggaca gtgaaaatac ttgtcaccaa
     5461 agtttcatgc agagcttact tgcccctcac ctcagtgatc aggtcattgg gagccagagg
     5521 tcactctcag aacatcagag gaatacacag tgtggtccat cctctgcaat tgaatataat
     5581 tgtcccccaa ctcatgaaaa tgtccatatt agaagagaga gtgagagtca gaatagggaa
     5641 agttgtgaca tgtcgttagg tgcaattaac accaggaaca gcaccttgaa tattcctttt
     5701 tcaagttcct cttcctcagg agatattcaa ggtcgaaaca caagccccaa tgtttctgta
     5761 cagaaatcca atcccatgag gattactgag agtcatgcca ccaagggcca catgaaccct
     5821 ccagtcacaa ccaacatgca tggggttgca aggccagcgt tgccacatcc atctgtgtct
     5881 catggaaatg gcgatcaagg ccctgctgta cgtcaagcta attcttcagt tccccagaga
     5941 tcaaggcatc ccctgcaaga cagcagtggt tccaaaattc gtcagcctga aaggaatcgt
     6001 tctggaaacc aaaggcaaag tactgtcttt gatccaagtc ttccccatct tcctctctct
     6061 actggtggca gtatgattct tggacgtcaa caacctgcca cagagaagag aggaagtatt
     6121 gttcgtttca tgcctgatag cccacaagta cctaatgata attcaggtcc tgaccagcat
     6181 acactatcac aaaattttgg tttttctttt attcctgagg gtggcatgaa tccaccaata
     6241 aatgctaatg cttctttcat tccacaggtt actcagccta gtgccactcg aactccagcc
     6301 ctcatcccgg tagatccgca aaatactctg ccctccttct atcctccata ctctcctgct
     6361 catcctacac tgtccaatga tatttcaatc ccctattttc ctaatcagat gttctcaaat
     6421 cctagcacag agaaggtaaa cagtggaagt ttaaataacc gatttggatc aattttatct
     6481 cctcccagac ctgttgggtt tgctcaacca agttttcctc ttctcccaga tatgccacca
     6541 atgcacatga ccaactctca cttatccaat tttaatatga catctttgtt tccagaaata
     6601 gccacagcgc ttcctgatgg ctcagcaatg tcacctttgc ttacaatagc aaattcctct
     6661 gcctctgact cttccaagca gtcctcaaac agacctgccc ataacataag ccatattcta
     6721 ggtcatgatt gcagttcagc tgtttagtaa tgaactagtc cgtaacttga aagtatttcg
     6781 atttcttggc tttatatatc ttgtggaaag gacgacaacc ttagacctaa ggaacggaa
//
