LOCUS       sequence-284697-        2558 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2558)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2558)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1812
FEATURES             Location/Qualifiers
     source          1..2558
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1999..2010
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 accgccacca tggatagtga atcaaacatc ccttctggaa caatacagag taggaaaggt
       61 ttgcagaata agagtcagtt taggaccatt gcaccaaaaa ttgtgcccaa agtcctaacg
      121 tccagaatgc tgccatgtca ttcaccatca cgctctgatc aggtgaatct gggaccctcc
      181 atcaactcca agctgctggg gatgtccacc cagaactatg ccctgatgca ggttgctggc
      241 caggagggga cattttctct tgttgctctg ccacatgttg cctcagctca gccaattcag
      301 aaacccagaa tgtccctacc tgaaaacctg aaacttccta ttcctagata tcaaccccct
      361 agaaatagca aagcatcaag aaagaaaccc atcctgatct ttcctaagag tggctgtagc
      421 aaagctcctg cccaaaccca aatgtgtcct cagatgtccc cttccccacc tcaccaccct
      481 gaactcctgt acaaacccag tccatttgag gaagtaccat cactagagca agccccagcc
      541 agcattagca cagctgcgct gaccaatgga agtgaccatg gggacttgag accaccagtg
      601 accaacaccc atggcagtct gaaccctcct gctaccccag catcatccac accagaggag
      661 cctgccaagc aggacctcac agctctttca gggaaagcac actttgtaag caagataaca
      721 tctagtaaac cttctgctgt tgccagtgaa aaatttaaag aacaagttga tcttgcaaaa
      781 accatgacca atttatcacc aaccattctt ggcaatgcag ttcagttgat ctcttcagtc
      841 cccaaaggga aactgccaat cccaccctac tcaagaatga agacaatgga ggtttacaaa
      901 atcaaatcag atgctaacat tgcaggtttt tctttaccag gacctaaggc cgactgtgat
      961 aagataccct ccaccacaga aggctttaat gcagccacca aggtggcaag caggctacct
     1021 gttccacaag tgtcacagca gagtgcctgt gaaagtgcct tttgtccacc caccaaactt
     1081 gatcttaacc acaaaacaaa actgaacagt ggagcagcaa agagaaaagg aagaaaacgg
     1141 aaggtaccag atgaaatttt ggcatttcag ggaaaaagga ggaaatatat cattaataag
     1201 tgtagagatg gtaaagaaag agtaaaaaat gatccccaag aattcagaga ccaaaagctg
     1261 gggaccctga aaaaataccg tagcattatg cccaaaccta tcatggtcat acccactttg
     1321 gcctccctgg cttctccaac tacactacag tcccagatgc ttgggggcct aggacaggat
     1381 gttttgttaa ataattcact cactcctaaa tatcttggct gtaagcaaga caacagctct
     1441 tcccctaagc ccagctccgt gttcagaaat ggattctctg gcattaagaa gccttggcac
     1501 agatgtcacg tctgcaacca ccacttccag ttcaaacagc accttcgaga ccacatgaat
     1561 acacacacca acagacgccc ttacagttgt cggatttgtc gcaagtccta tgtacgtcct
     1621 ggcagcctga gcacacacat gaaacttcat catggtgaga accgtctgaa gaaactcatg
     1681 tgttgtgagt tttgtgcaaa agtgtttggc cacatccgag tctattttgg ccatctgaaa
     1741 gaagtgcata gggttgtgat cagcactgag cctgcgccca gtgaactgca gccaggagac
     1801 ataccaaaga acagagacat gagtgtgcga ggcatggagg gatcattgga gagggaaaac
     1861 aagtccaacc tggaagaaga cttccttcta aaccaggcag acgaagtcaa attacaaatc
     1921 aaatgtggtc gttgtcagat tactgctcag tcttttgcgg aaataaaatt tcatttactt
     1981 gatgttcatg gagaggaaat tgagggcagg ctacaagaag ggaccttccc aggaagcaag
     2041 gggactcagg aagagttggt gcagcacgct agccccgact ggaaaaggca tcctgagaga
     2101 gggaagccgg agaaggttca ttcctcctcc gaggaatcac atgcatgtcc aagactgaaa
     2161 aggcagctcc accttcatca gaatggcgtg gaaatgctca tggaaaatga aggaccccag
     2221 tcaggaacca acaagccaag ggaaacctgc cagggccctg agtgtcctgg cctccacacg
     2281 tttctcttgt ggtcccattc aggctttaac tgcctgcttt gtgcagagat gctgggacgg
     2341 aaagaggacc tcctccacca ctggaagcac cagcataact gtgaggaccc ttccaaactg
     2401 tgggctattt taaatacggt ctccaaccag ggagtgatcg aactttccag tgaagctgag
     2461 aaatagtaat gaactagtcc gtaacttgaa agtatttcga tttcttggct ttatatatct
     2521 tgtggaaagg acgataatca ctattagacc actgaggg
//
