LOCUS       sequence-284706-        8030 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8030)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 8030)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1889
FEATURES             Location/Qualifiers
     source          1..8030
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      6245..6254
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tgtctggcgg cgccgcagag aagcagagca gcactcccgg ttccctgttc
       61 ctctcgccgc cggctcctgc ccccaagaat ggctccagct ccgattcctc cgtgggggag
      121 aaactgggag ccgcggccgc cgacgctgtg accggcagga ccgaggagta caggcgccgc
      181 cgccacacta tggacaagga cagccgtggg gcggccgcga ccactaccac cactgagcac
      241 cgcttcttcc gccggagcgt catctgtgac tccaatgcca ctgcactgga gcttcccggc
      301 cttcctcttt ccctgcccca gcccagcatc cccgcggctg tcccgcagag tgctccaccg
      361 gagccccacc gggaagagac cgtgaccgcc accgccactt cccaggtagc ccagcagcct
      421 ccagccgctg ccgcccctgg ggaacaggcc gtcgcgggcc ctgccccctc gactgtcccc
      481 agcagtacca gcaaagaccg cccagtgtcc cagcctagcc ttgtggggag caaagaggag
      541 ccgccgccgg cgagaagtgg cagcggcggc ggcagcgcca aggagccaca ggaggaacgg
      601 agccagcagc aggatgatat cgaagagctg gagaccaagg ccgtgggaat gtctaacgat
      661 ggccgctttc tcaagtttga catcgaaatc ggcagaggct cctttaagac ggtctacaaa
      721 ggtctggaca ctgaaaccac cgtggaagtc gcctggtgtg aactgcagga tcgaaaatta
      781 acaaagtctg agaggcagag atttaaagaa gaagctgaaa tgttaaaagg tcttcagcat
      841 cccaatattg ttagatttta tgattcctgg gaatccacag taaaaggaaa gaagtgcatt
      901 gttttggtga ctgaacttat gacgtctgga acacttaaaa cgtatctgaa aaggtttaaa
      961 gtgatgaaga tcaaagttct aagaagctgg tgccgtcaga tccttaaagg tcttcagttt
     1021 cttcatactc gaactccacc tatcattcac cgcgatctta aatgtgacaa catctttatc
     1081 accggcccta ctggctcagt caagattgga gacctcggtc tggcaaccct gaagcgggct
     1141 tcttttgcca agagtgtgat aggtacccca gagttcatgg cccctgagat gtatgaggag
     1201 aaatatgatg aatccgttga cgtttatgct tttgggatgt gcatgcttga gatggctaca
     1261 tctgaatatc cttactcgga gtgccaaaat gctgcacaga tctaccgtcg cgtgaccagt
     1321 ggggtgaagc cagccagttt tgacaaagta gcaattcctg aagtgaagga aattattgaa
     1381 ggatgcatac gacaaaacaa agatgaaaga tattccatca aagacctttt gaaccatgcc
     1441 ttcttccaag aggaaacagg agtacgggta gaattagcag aagaagatga tggagaaaaa
     1501 atagccataa aattatggct acgtattgaa gatattaaga aattaaaggg aaaatacaaa
     1561 gataatgaag ctattgagtt ttcttttgat ttagagagag atgtcccaga agatgttgca
     1621 caagaaatgg tagagtctgg gtatgtctgt gaaggtgatc acaagaccat ggctaaagct
     1681 atcaaagaca gagtatcatt aattaagagg aaacgagagc agcggcagtt ggtacgggag
     1741 gagcaagaaa aaaaaaagca ggaagagagc agtctcaaac agcaggtaga acaatccagt
     1801 gcttcccaga caggaatcaa gcagctccct tctgctagca ccggcatacc tactgcttct
     1861 accacttcag cttcagtttc tacacaagta gaacctgaag aacctgaggc agatcaacat
     1921 caacaactac agtaccagca acccagtata tctgtgttat ctgatgggac ggttgacagt
     1981 ggtcagggat cctctgtctt cacagaatct cgagtgagca gccaacagac agtttcatat
     2041 ggttcccaac atgaacaggc acattctaca ggcacagtcc cagggcatat accttctact
     2101 gtccaagcac agtctcagcc ccatggggta tatccaccct caagtgtgcc tcagtccatg
     2161 gcgcatccgt gtggggggac cccaacatac ccagaatcac agatattttt cccaactatt
     2221 catgaacgtc cagtttcttt ttcaccacct cccacctgcc caccgaaagt agccatttcc
     2281 cagcggcgta agagcacctc cttcctggaa gcccaaactc accacttcca acccctgctg
     2341 aggactgttg gccaaagtct tcttccacct ggtggcagcc caactaactg gacaccagag
     2401 gccgtagtta tgttgggtac tacagccagt agagtaactg gagagtcatg tgagatacag
     2461 gtccatccta tgtttgaacc atctcaagtt tacagtgact atagacctgg actagtactt
     2521 ccagaagaag ctcactattt tattcctcag gaagcagtgt atgtagctgg ggtacattac
     2581 caggcccggg tggcagaaca gtatgagggc attccataca actcatcagt actgtcaagt
     2641 cctatgaaac agatacctga acagaagcca gtacaagggg gccctacttc aagttctgtc
     2701 tttgaatttc catctggaca ggctttcctg gtaggacacc ttcagaatct aagattagat
     2761 tctggattgg gtccgggatc tcccctctct agtatttctg cacctatcag tacagatgct
     2821 acacgtttga aatttcaccc tgtctttgtt cctcattctg cgcctgctgt gttaactcat
     2881 aacaatgaga gcagaagcaa ctgtgtattt gaatttcatg ttcacacacc aagctcctct
     2941 tcaggagaag gaggtggaat tttacctcag cgtgtttacc gaaatcggca ggttgcagtg
     3001 gacttgaatc aagaagaact gcctcctcaa tcagttggat tacatggcta cttgcagcct
     3061 gtgactgaag aaaagcataa ttaccatgcc ccagaattga ccgtttctgt ggtagagcct
     3121 atcggacaga actggccaat aggaagccca gaatattcca gtgattcctc acaaatcact
     3181 tcttcagacc ccagtgattt tcagtcacct ccccctacag ggggagcagc tgcacctttt
     3241 ggctctgacg tctcaatgcc ctttatccat ctgcctcaga cagtgttaca agaatcccca
     3301 cttttcttct gtttccccca aggaaccaca tctcagcagg tcttaactgc ctcattttct
     3361 tcaggaggat ctgcacttca tccacaggca caggggcaga gccagggtca gccatcctca
     3421 agtagcttaa caggggtttc atcttcccaa cccatacaac atcctcagca gcagcaggga
     3481 atacagcaga cagcccctcc tcaacagaca gtgcagtatt cactttcaca gacatcaacc
     3541 tccagtgagg ccactactgc acagccagtg agtcagcctc aagctccaca agtcttgcct
     3601 caagtatcag ctggaaaaca gggcttccca cctcgactgc caccacagta cccaggagat
     3661 tcaaatattg ctccctcttc caacgtggct tctgtttgca tccattctac agtcctatcc
     3721 cctcccatgc cgacagaagt actggctaca cctgggtact ttcccacagt ggtgcagcct
     3781 tatgtggaat caaatctttt agttcctatg ggtggtgtag gaggacaggt tcaagtgtcc
     3841 cagccaggag ggagtttagc acaagccccc actacatcct cccagcaagc agttttggag
     3901 agtactcagg gagtctctca ggttgctcct gcagagccag ttgcagtagc acagacccaa
     3961 gctacccagc cgaccacttt ggcttcctct gtagacagtg cacattcaga tgttgcttca
     4021 ggtatgagtg atggcaatga gaacgtccca tcttccagtg gaaggcatga aggaagaact
     4081 acaaaacggc attaccgaaa atctgtaagg agtcgctctc gacatgaaaa aacttcacgc
     4141 ccaaaattaa gaattttgaa tgtttcaaat aaaggagacc gagtagtaga atgtcaatta
     4201 gagactcata ataggaaaat ggttacattc aaatttgacc tagatggtga caaccccgag
     4261 gagatagcaa caattatggt gaacaatgac tttattctag caatagagag agagtcgttt
     4321 gtggatcaag tgcgagaaat tattgaaaaa gctgatgaaa tgctcagtga ggatgtcagt
     4381 gtggaaccag agggtgatca gggattggag agtctacaag gaaaggatga ctatggcttt
     4441 tcaggttctc agaaattgga aggagagttc aaacaaccaa ttcctgcgtc ttccatgcca
     4501 cagcaaatag gcattcctac cagttcttta actcaagttg ttcattctgc gggaaggcgg
     4561 tttatagtga gtcctgtgcc agaaagccga ttacgagaat caaaagtttt ccccagtgaa
     4621 ataacagata cagttgctgc ctctacagct cagagccctg gaatgaactt gtctcactct
     4681 gcatcatccc ttagtctaca acaggccttt tctgaactta gacgtgccca aatgacagaa
     4741 ggacccaaca cagcacctcc aaactttagt catacaggac caacatttcc agtagtacct
     4801 cctttcttaa gtagcattgc tggagtccca accacagcag cagccacagc accagtccct
     4861 gcaacaagca gccctcctaa tgacatttcc acatcagtaa ttcagtctga ggttacagtg
     4921 cccactgaag aggggattgc tggagttgcc accagcacag gtgtggtaac ttcaggtggt
     4981 ctccccatac cacctgtgtc tgaatcacca gtactttcca gcgtagtttc aagtatcaca
     5041 atacctgcag ttgtctcaat atctactaca tccccgtcac ttcaagtccc cacatccaca
     5101 tctgagatcg ttgtttctag tacagcactg tatccttcag taacagtttc agcaacttca
     5161 gcctctgcag ggggcagtac tgctacccca ggtcctaagc ctccagctgt agtatctcag
     5221 caggcagcag gcagcactac tgtgggagcc acattaacat cagtttctac caccacttca
     5281 ttcccaagca cagcttcaca gctgtgcatt cagcttagca gcagtacttc tactcctact
     5341 ttagctgaaa ccgtggtagt tagcgcacac tcactagata agacatctca tagcagtaca
     5401 actggattgg ctttctccct ctctgcacca tcttcctctt cctctcctgg agcaggagtg
     5461 tctagttata tttctcagcc tggtgggctg catcctttgg tcattccatc agtgatagct
     5521 tctactccta ttcttcccca agcagcagga cctacttcta cacctttatt accccaagta
     5581 cctagtatcc cacccttggt acagcctgtt gccaatgtgc ctgctgtaca gcagacacta
     5641 attcatagtc agcctcaacc agctttgctt cccaaccagc cccatactca ttgtcctgaa
     5701 gtagattctg atacacaacc caaagctcct ggaattgatg acataaagac tctagaagaa
     5761 aagctgcggt ctctgttcag tgaacacagc tcatctggag ctcagcatgc ctctgtctca
     5821 ctggagacct cactagtcat agagagcact gtcacaccag gcatcccaac tactgctgtt
     5881 gcaccaagca aactcctgac ttctaccaca agtacttgct taccaccaac caatttacca
     5941 ctaggaacag ttgctttgcc agttacacca gtggtcacac ctgggcaagt ttctacccca
     6001 gtcagcacta ctacatcagg agtgaaacct ggaactgctc cctccaagcc acctctaact
     6061 aaggctccgg tgctgccagt gggtactgaa cttccagcag gtactctacc cagcgagcag
     6121 ctgccacctt ttccaggacc ttctctaacc cagtcccagc aacctctaga ggatcttgat
     6181 gctcaattga gaagaacact tagtccagag atgatcacag tgacttctgc ggttggtcct
     6241 gtgtccatgg cggctccaac agcaatcaca gaagcaggaa cacagcctca gaagggtgtt
     6301 tctcaagtca aagaaggccc tgtcctagca actagttcag gagctggtgt ttttaagatg
     6361 ggacgatttc aggtttctgt tgcagcagac ggtgcccaga aagagggtaa aaataagtca
     6421 gaagatgcaa agtctgttca ttttgaatcc agcacctcag agtcctcagt gctatcaagt
     6481 agtagtccag agagtacctt ggtgaaacca gagccgaatg gcataaccat ccctggtatc
     6541 tcttcagatg tgccagagag tgcccacaaa actactgcct cagaggcaaa gtcagacact
     6601 gggcagccta ccaaggttgg acgttttcag gtgacaacta cagcaaacaa agtgggtcgt
     6661 ttctctgtat caaaaactga ggacaagatc actgacacaa agaaagaagg accagtggca
     6721 tctcctcctt ttatggattt ggaacaagct gttcttcctg ctgtgatacc aaagaaagag
     6781 aagcctgaac tgtcagagcc ttcacatcta aatgggccgt cttctgaccc ggaggccgct
     6841 tttttaagta gggatgtgga tgatggttcc ggtagtccac actcgcccca tcagctgagc
     6901 tcaaagagcc ttcctagcca gaatctaagt caaagcctta gtaattcatt taactcctct
     6961 tacatgagta gcgacaatga gtcagatatc gaagatgaag acttaaagtt agagctgcga
     7021 cgactacgag ataaacatct caaagagatt caggacctgc agagtcgcca gaagcatgaa
     7081 attgaatctt tgtataccaa actgggcaag gtgccccctg ctgttattat tcccccagct
     7141 gctccccttt cagggagaag acgacgaccc actaaaagca aaggcagcaa atctagtcga
     7201 agcagttcct tggggaataa aagcccccag ctttcaggta acctgtctgg tcagagtgca
     7261 gcttcagtct tgcaccccca gcagaccctc caccctcctg gcaacatccc agagtccggg
     7321 cagaatcagc tgttacagcc ccttaagcca tctccctcca gtgacaacct ctattcagcc
     7381 ttcaccagtg atggtgccat ttcagtacca agcctttctg ctccaggtca aggaaccagc
     7441 agcacaaaca ctgttggggc aacagtgaac agccaagccg cccaagctca gcctcctgcc
     7501 atgacgtcca gcaggaaggg cacattcaca gatgacttgc acaagttggt agacaattgg
     7561 gcccgagatg ccatgaatct ctcaggcagg agaggaagca aagggcacat gaattacgag
     7621 ggccctggaa tggcaaggaa gttctctgca cctgggcaac tgtgcatctc catgacctcg
     7681 aacctgggtg gctctgcccc catctctgca gcatcagcta cctctctagg tcacttcacc
     7741 aagtctatgt gccccccaca gcagtatggc tttccagcta ccccatttgg cgctcaatgg
     7801 agtgggacgg gtggcccagc accacagcca cttggccagt tccaacctgt gggaactgcc
     7861 tccttgcaga atttcaacat cagcaatttg cagaaatcca tcagcaaccc cccaggctcc
     7921 aacctgcgga ccacttagta atgaactagt ccgtaacttg aaagtatttc gatttcttgg
     7981 ctttatatat cttgtggaaa ggacgaccga cggtgcgacg tctttcgttg
//
