LOCUS       sequence-284709-        6506 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6506)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 6506)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1890
FEATURES             Location/Qualifiers
     source          1..6506
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4724..4733
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tgtctggcgg cgccgcagag aagcagagca gcactcccgg ttccctgttc
       61 ctctcgccgc cggctcctgc ccccaagaat ggctccagct ccgattcctc cgtgggggag
      121 aaactgggag ccgcggccgc cgacgctgtg accggcagga ccgaggagta caggcgccgc
      181 cgccacacta tggacaagga cagccgtggg gcggccgcga ccactaccac cactgagcac
      241 cgcttcttcc gccggagcgt catctgtgac tccaatgcca ctgcactgga gcttcccggc
      301 cttcctcttt ccctgcccca gcccagcatc cccgcggctg tcccgcagag tgctccaccg
      361 gagccccacc gggaagagac cgtgaccgcc accgccactt cccaggtagc ccagcagcct
      421 ccagccgctg ccgcccctgg ggaacaggcc gtcgcgggcc ctgccccctc gactgtcccc
      481 agcagtacca gcaaagaccg cccagtgtcc cagcctagcc ttgtggggag caaagaggag
      541 ccgccgccgg cgagaagtgg cagcggcggc ggcagcgcca aggagccaca ggaggaacgg
      601 agccagcagc aggatgatat cgaagagctg gagaccaagg ccgtgggaat gtctaacgat
      661 ggccgctttc tcaagtttga catcgaaatc ggcagaggct cctttaagac ggtctacaaa
      721 ggtctggaca ctgaaaccac cgtggaagtc gcctggtgtg aactgcagga tcgaaaatta
      781 acaaagtctg agaggcagag atttaaagaa gaagctgaaa tgttaaaagg tcttcagcat
      841 cccaatattg ttagatttta tgattcctgg gaatccacag taaaaggaaa gaagtgcatt
      901 gttttggtga ctgaacttat gacgtctgga acacttaaaa cgtatctgaa aaggtttaaa
      961 gtgatgaaga tcaaagttct aagaagctgg tgccgtcaga tccttaaagg tcttcagttt
     1021 cttcatactc gaactccacc tatcattcac cgcgatctta aatgtgacaa catctttatc
     1081 accggcccta ctggctcagt caagattgga gacctcggtc tggcaaccct gaagcgggct
     1141 tcttttgcca agagtgtgat aggtacccca gagttcatgg cccctgagat gtatgaggag
     1201 aaatatgatg aatccgttga cgtttatgct tttgggatgt gcatgcttga gatggctaca
     1261 tctgaatatc cttactcgga gtgccaaaat gctgcacaga tctaccgtcg cgtgaccagt
     1321 ggggtgaagc cagccagttt tgacaaagta gcaattcctg aagtgaagga aattattgaa
     1381 ggatgcatac gacaaaacaa agatgaaaga tattccatca aagacctttt gaaccatgcc
     1441 ttcttccaag aggaaacagg agtacgggta gaattagcag aagaagatga tggagaaaaa
     1501 atagccataa aattatggct acgtattgaa gatattaaga aattaaaggg aaaatacaaa
     1561 gataatgaag ctattgagtt ttcttttgat ttagagagag atgtcccaga agatgttgca
     1621 caagaaatgg tagagtctgg gtatgtctgt gaaggtgatc acaagaccat ggctaaagct
     1681 atcaaagaca gagtatcatt aattaagagg aaacgagagc agcggcagtt ggtacgggag
     1741 gagcaagaaa aaaaaaagca ggaagagagc agtctcaaac agcaggtaga acaatccagt
     1801 gcttcccaga caggaatcaa gcagctccct tctgctagca ccggcatacc tactgcttct
     1861 accacttcag cttcagtttc tacacaagta gaacctgaag aacctgaggc agatcaacat
     1921 caacaactac agtaccagca acccagtata tctgtgttat ctgatgggac ggttgacagt
     1981 ggtcagggat cctctgtctt cacagaatct cgagtgagca gccaacagac agtttcatat
     2041 ggttcccaac atgaacaggc acattctaca ggcacagtcc cagggcatat accttctact
     2101 gtccaagcac agtctcagcc ccatggggta tatccaccct caagtgtggc acaggggcag
     2161 agccagggtc agccatcctc aagtagctta acaggggttt catcttccca acccatacaa
     2221 catcctcagc agcagggaat acagcagaca gcccctcctc aacagacagt gcagtattca
     2281 ctttcacaga catcaacctc cagtgaggcc actactgcac agccagtgag tcagcctcaa
     2341 gctccacaag tcttgcctca agtatcagct ggaaaacaga gtactcaggg agtctctcag
     2401 gttgctcctg cagagccagt tgcagtagca cagacccaag ctacccagcc gaccactttg
     2461 gcttcctctg tagacagtgc acattcagat gttgcttcag gtatgagtga tggcaatgag
     2521 aacgtcccat cttccagtgg aaggcatgaa ggaagaacta caaaacggca ttaccgaaaa
     2581 tctgtaagga gtcgctctcg acatgaaaaa acttcacgcc caaaattaag aattttgaat
     2641 gtttcaaata aaggagaccg agtagtagaa tgtcaattag agactcataa taggaaaatg
     2701 gttacattca aatttgacct agatggtgac aaccccgagg agatagcaac aattatggtg
     2761 aacaatgact ttattctagc aatagagaga gagtcgtttg tggatcaagt gcgagaaatt
     2821 attgaaaaag ctgatgaaat gctcagtgag gatgtcagtg tggaaccaga gggtgatcag
     2881 ggattggaga gtctacaagg aaaggatgac tatggctttt caggttctca gaaattggaa
     2941 ggagagttca aacaaccaat tcctgcgtct tccatgccac agcaaatagg cattcctacc
     3001 agttctttaa ctcaagttgt tcattctgcg ggaaggcggt ttatagtgag tcctgtgcca
     3061 gaaagccgat tacgagaatc aaaagttttc cccagtgaaa taacagatac agttgctgcc
     3121 tctacagctc agagccctgg aatgaacttg tctcactctg catcatccct tagtctacaa
     3181 caggcctttt ctgaacttag acgtgcccaa atgacagaag gacccaacac agcacctcca
     3241 aactttagtc atacaggacc aacatttcca gtagtacctc ctttcttaag tagcattgct
     3301 ggagtcccaa ccacagcagc agccacagca ccagtccctg caacaagcag ccctcctaat
     3361 gacatttcca catcagtaat tcagtctgag gttacagtgc ccactgaaga ggggattgct
     3421 ggagttgcca ccagcacagg tgtggtaact tcaggtggtc tccccatacc acctgtgtct
     3481 gaatcaccag tactttccag cgtagtttca agtatcacaa tacctgcagt tgtctcaata
     3541 tctactacat ccccgtcact tcaagtcccc acatccacat ctgagatcgt tgtttctagt
     3601 acagcactgt atccttcagt aacagtttca gcaacttcag cctctgcagg gggcagtact
     3661 gctaccccag gtcctaagcc tccagctgta gtatctcagc aggcagcagg cagcactact
     3721 gtgggagcca cattaacatc agtttctacc accacttcat tcccaagcac agcttcacag
     3781 ctgtgcattc agcttagcag cagtacttct actcctactt tagctgaaac cgtggtagtt
     3841 agcgcacact cactagataa gacatctcat agcagtacaa ctggattggc tttctccctc
     3901 tctgcaccat cttcctcttc ctctcctgga gcaggagtgt ctagttatat ttctcagcct
     3961 ggtgggctgc atcctttggt cattccatca gtgatagctt ctactcctat tcttccccaa
     4021 gcagcaggac ctacttctac acctttatta ccccaagtac ctagtatccc acccttggta
     4081 cagcctgttg ccaatgtgcc tgctgtacag cagacactaa ttcatagtca gcctcaacca
     4141 gctttgcttc ccaaccagcc ccatactcat tgtcctgaag tagattctga tacacaaccc
     4201 aaagctcctg gaattgatga cataaagact ctagaagaaa agctgcggtc tctgttcagt
     4261 gaacacagct catctggagc tcagcatgcc tctgtctcac tggagacctc actagtcata
     4321 gagagcactg tcacaccagg catcccaact actgctgttg caccaagcaa actcctgact
     4381 tctaccacaa gtacttgctt accaccaacc aatttaccac taggaacagt tgctttgcca
     4441 gttacaccag tggtcacacc tgggcaagtt tctaccccag tcagcactac tacatcagga
     4501 gtgaaacctg gaactgctcc ctccaagcca cctctaacta aggctccggt gctgccagtg
     4561 ggtactgaac ttccagcagg tactctaccc agcgagcagc tgccaccttt tccaggacct
     4621 tctctaaccc agtcccagca acctctagag gatcttgatg ctcaattgag aagaacactt
     4681 agtccagaga tgatcacagt gacttctgcg gttggtcctg tgtccatggc ggctccaaca
     4741 gcaatcacag aagcaggaac acagcctcag aaggtttctc aagtcaaaga aggccctgtc
     4801 ctagcaacta gttcaggagc tggtgttttt aagatgggac gatttcaggt ttctgttgca
     4861 gcagacggtg cccagaaaga gggtaaaaat aagtcagaag atgcaaagtc tgttcatttt
     4921 gaatccagca cctcagagtc ctcagtgcta tcaagtagta gtccagagag taccttggtg
     4981 aaaccagagc cgaatggcat aaccatccct ggtatctctt cagatgtgcc agagagtgcc
     5041 cacaaaacta ctgcctcaga ggcaaagtca gacactgggc agcctaccaa ggttggacgt
     5101 tttcaggtga caactacagc aaacaaagtg ggtcgtttct ctgtatcaaa aactgaggac
     5161 aagatcactg acacaaagaa agaaggacca gtggcatctc ctccttttat ggatttggaa
     5221 caagctgttc ttcctgctgt gataccaaag aaagagaagc ctgaactgtc agagccttca
     5281 catctaaatg ggccgtcttc tgacccggag gccgcttttt taagtaggga tgtggatgat
     5341 ggttccggta gtccacactc gccccatcag ctgagctcaa agagccttcc tagccagaat
     5401 ctaagtcaaa gccttagtaa ttcatttaac tcctcttaca tgagtagcga caatgagtca
     5461 gatatcgaag atgaagactt aaagttagag ctgcgacgac tacgagataa acatctcaaa
     5521 gagattcagg acctgcagag tcgccagaag catgaaattg aatctttgta taccaaactg
     5581 ggcaaggtgc cccctgctgt tattattccc ccagctgctc ccctttcagg gagaagacga
     5641 cgacccacta aaagcaaagg cagcaaatct agtcgaagca gttccttggg gaataaaagc
     5701 ccccagcttt caggtaacct gtctggtcag agtgcagctt cagtcttgca cccccagcag
     5761 accctccacc ctcctggcaa catcccagag tccgggcaga atcagctgtt acagcccctt
     5821 aagccatctc cctccagtga caacctctat tcagccttca ccagtgatgg tgccatttca
     5881 gtaccaagcc tttctgctcc aggtcaagga accagcagca caaacactgt tggggcaaca
     5941 gtgaacagcc aagccgccca agctcagcct cctgccatga cgtccagcag gaagggcaca
     6001 ttcacagatg acttgcacaa gttggtagac aattgggccc gagatgccat gaatctctca
     6061 ggcaggagag gaagcaaagg gcacatgaat tacgagggcc ctggaatggc aaggaagttc
     6121 tctgcacctg ggcaactgtg catctccatg acctcgaacc tgggtggctc tgcccccatc
     6181 tctgcagcat cagctacctc tctaggtcac ttcaccaagt ctatgtgccc cccacagcag
     6241 tatggctttc cagctacccc atttggcgct caatggagtg ggacgggtgg cccagcacca
     6301 cagccacttg gccagttcca acctgtggga actgcctcct tgcagaattt caacatcagc
     6361 aatttgcaga aatccatcag caacccccca ggctccaacc tgcggaccac ttagtaatga
     6421 actagtccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac
     6481 gatcagttgc tgtgcgcctt atccgt
//
