LOCUS       sequence-284712-        7250 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 7250)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 7250)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1891
FEATURES             Location/Qualifiers
     source          1..7250
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      5465..5474
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tgtctggcgg cgccgcagag aagcagagca gcactcccgg ttccctgttc
       61 ctctcgccgc cggctcctgc ccccaagaat ggctccagct ccgattcctc cgtgggggag
      121 aaactgggag ccgcggccgc cgacgctgtg accggcagga ccgaggagta caggcgccgc
      181 cgccacacta tggacaagga cagccgtggg gcggccgcga ccactaccac cactgagcac
      241 cgcttcttcc gccggagcgt catctgtgac tccaatgcca ctgcactgga gcttcccggc
      301 cttcctcttt ccctgcccca gcccagcatc cccgcggctg tcccgcagag tgctccaccg
      361 gagccccacc gggaagagac cgtgaccgcc accgccactt cccaggtagc ccagcagcct
      421 ccagccgctg ccgcccctgg ggaacaggcc gtcgcgggcc ctgccccctc gactgtcccc
      481 agcagtacca gcaaagaccg cccagtgtcc cagcctagcc ttgtggggag caaagaggag
      541 ccgccgccgg cgagaagtgg cagcggcggc ggcagcgcca aggagccaca ggaggaacgg
      601 agccagcagc aggatgatat cgaagagctg gagaccaagg ccgtgggaat gtctaacgat
      661 ggccgctttc tcaagtttga catcgaaatc ggcagaggct cctttaagac ggtctacaaa
      721 ggtctggaca ctgaaaccac cgtggaagtc gcctggtgtg aactgcagga tcgaaaatta
      781 acaaagtctg agaggcagag atttaaagaa gaagctgaaa tgttaaaagg tcttcagcat
      841 cccaatattg ttagatttta tgattcctgg gaatccacag taaaaggaaa gaagtgcatt
      901 gttttggtga ctgaacttat gacgtctgga acacttaaaa cgtatctgaa aaggtttaaa
      961 gtgatgaaga tcaaagttct aagaagctgg tgccgtcaga tccttaaagg tcttcagttt
     1021 cttcatactc gaactccacc tatcattcac cgcgatctta aatgtgacaa catctttatc
     1081 accggcccta ctggctcagt caagattgga gacctcggtc tggcaaccct gaagcgggct
     1141 tcttttgcca agagtgtgat aggtacccca gagttcatgg cccctgagat gtatgaggag
     1201 aaatatgatg aatccgttga cgtttatgct tttgggatgt gcatgcttga gatggctaca
     1261 tctgaatatc cttactcgga gtgccaaaat gctgcacaga tctaccgtcg cgtgaccagt
     1321 ggggtgaagc cagccagttt tgacaaagta gcaattcctg aagtgaagga aattattgaa
     1381 ggatgcatac gacaaaacaa agatgaaaga tattccatca aagacctttt gaaccatgcc
     1441 ttcttccaag aggaaacagg agtacgggta gaattagcag aagaagatga tggagaaaaa
     1501 atagccataa aattatggct acgtattgaa gatattaaga aattaaaggg aaaatacaaa
     1561 gataatgaag ctattgagtt ttcttttgat ttagagagag atgtcccaga agatgttgca
     1621 caagaaatgg tagagtctgg gtatgtctgt gaaggtgatc acaagaccat ggctaaagct
     1681 atcaaagaca gagtatcatt aattaagagg aaacgagagc agcggcagtt ggtacgggag
     1741 gagcaagaaa aaaaaaagca ggaagagagc agtctcaaac agcaggtaga acaatccagt
     1801 gcttcccaga caggaatcaa gcagctccct tctgctagca ccggcatacc tactgcttct
     1861 accacttcag cttcagtttc tacacaagta gaacctgaag aacctgaggc agatcaacat
     1921 caacaactac agtaccagca acccagtata tctgtgttat ctgatgggac ggttgacagt
     1981 ggtcagggat cctctgtctt cacagaatct cgagtgagca gccaacagac agtttcatat
     2041 ggttcccaac atgaacaggc acattctaca ggcacagtcc cagggcatat accttctact
     2101 gtccaagcac agtctcagcc ccatggggta tatccaccct caagtgtggc acaggggcag
     2161 agccagggtc agccatcctc aagtagctta acaggggttt catcttccca acccatacaa
     2221 catcctcagc agcagcaggg aatacagcag acagcccctc ctcaacagac agtgcagtat
     2281 tcactttcac agacatcaac ctccagtgag gccactactg cacagccagt gagtcagcct
     2341 caagctccac aagtcttgcc tcaagtatca gctggaaaac agcttccagt ttcccagcca
     2401 gtaccaacta tccaaggcga acctcagatc ccagttgcga cacaaccctc ggttgttcca
     2461 gtccactctg gtgctcattt ccttccagtg ggacagccgc tccctactcc cttgctccct
     2521 cagtaccctg tctctcagat tcccatatca actcctcatg tgtctacggc tcagacaggt
     2581 ttctcatccc ttcccatcac aatggcagct ggcattactc agcctctgct cacgttggct
     2641 tcatctgcta caacagctgc gatcccgggg gtatcaactg tggttcctag tcagcttcca
     2701 acccttctgc agcctgtgac tcagctgcca agtcaggttc acccacagct cctacaacca
     2761 gcagttcagt ccatgggaat accagctaac cttggacaag ctgctgaggt tccactttcc
     2821 tctggagatg ttctgtacca gggcttccca cctcgactgc caccacagta cccaggagat
     2881 tcaaatattg ctccctcttc caacgtggct tctgtttgca tccattctac agtcctatcc
     2941 cctcccatgc cgacagaagt actggctaca cctgggtact ttcccacagt ggtgcagcct
     3001 tatgtggaat caaatctttt agttcctatg ggtggtgtag gaggacaggt tcaagtgtcc
     3061 cagccaggag ggagtttagc acaagccccc actacatcct cccagcaagc agttttggag
     3121 agtactcagg gagtctctca ggttgctcct gcagagccag ttgcagtagc acagacccaa
     3181 gctacccagc cgaccacttt ggcttcctct gtagacagtg cacattcaga tgttgcttca
     3241 ggtatgagtg atggcaatga gaacgtccca tcttccagtg gaaggcatga aggaagaact
     3301 acaaaacggc attaccgaaa atctgtaagg agtcgctctc gacatgaaaa aacttcacgc
     3361 ccaaaattaa gaattttgaa tgtttcaaat aaaggagacc gagtagtaga atgtcaatta
     3421 gagactcata ataggaaaat ggttacattc aaatttgacc tagatggtga caaccccgag
     3481 gagatagcaa caattatggt gaacaatgac tttattctag caatagagag agagtcgttt
     3541 gtggatcaag tgcgagaaat tattgaaaaa gctgatgaaa tgctcagtga ggatgtcagt
     3601 gtggaaccag agggtgatca gggattggag agtctacaag gaaaggatga ctatggcttt
     3661 tcaggttctc agaaattgga aggagagttc aaacaaccaa ttcctgcgtc ttccatgcca
     3721 cagcaaatag gcattcctac cagttcttta actcaagttg ttcattctgc gggaaggcgg
     3781 tttatagtga gtcctgtgcc agaaagccga ttacgagaat caaaagtttt ccccagtgaa
     3841 ataacagata cagttgctgc ctctacagct cagagccctg gaatgaactt gtctcactct
     3901 gcatcatccc ttagtctaca acaggccttt tctgaactta gacgtgccca aatgacagaa
     3961 ggacccaaca cagcacctcc aaactttagt catacaggac caacatttcc agtagtacct
     4021 cctttcttaa gtagcattgc tggagtccca accacagcag cagccacagc accagtccct
     4081 gcaacaagca gccctcctaa tgacatttcc acatcagtaa ttcagtctga ggttacagtg
     4141 cccactgaag aggggattgc tggagttgcc accagcacag gtgtggtaac ttcaggtggt
     4201 ctccccatac cacctgtgtc tgaatcacca gtactttcca gcgtagtttc aagtatcaca
     4261 atacctgcag ttgtctcaat atctactaca tccccgtcac ttcaagtccc cacatccaca
     4321 tctgagatcg ttgtttctag tacagcactg tatccttcag taacagtttc agcaacttca
     4381 gcctctgcag ggggcagtac tgctacccca ggtcctaagc ctccagctgt agtatctcag
     4441 caggcagcag gcagcactac tgtgggagcc acattaacat cagtttctac caccacttca
     4501 ttcccaagca cagcttcaca gctgtgcatt cagcttagca gcagtacttc tactcctact
     4561 ttagctgaaa ccgtggtagt tagcgcacac tcactagata agacatctca tagcagtaca
     4621 actggattgg ctttctccct ctctgcacca tcttcctctt cctctcctgg agcaggagtg
     4681 tctagttata tttctcagcc tggtgggctg catcctttgg tcattccatc agtgatagct
     4741 tctactccta ttcttcccca agcagcagga cctacttcta cacctttatt accccaagta
     4801 cctagtatcc cacccttggt acagcctgtt gccaatgtgc ctgctgtaca gcagacacta
     4861 attcatagtc agcctcaacc agctttgctt cccaaccagc cccatactca ttgtcctgaa
     4921 gtagattctg atacacaacc caaagctcct ggaattgatg acataaagac tctagaagaa
     4981 aagctgcggt ctctgttcag tgaacacagc tcatctggag ctcagcatgc ctctgtctca
     5041 ctggagacct cactagtcat agagagcact gtcacaccag gcatcccaac tactgctgtt
     5101 gcaccaagca aactcctgac ttctaccaca agtacttgct taccaccaac caatttacca
     5161 ctaggaacag ttgctttgcc agttacacca gtggtcacac ctgggcaagt ttctacccca
     5221 gtcagcacta ctacatcagg agtgaaacct ggaactgctc cctccaagcc acctctaact
     5281 aaggctccgg tgctgccagt gggtactgaa cttccagcag gtactctacc cagcgagcag
     5341 ctgccacctt ttccaggacc ttctctaacc cagtcccagc aacctctaga ggatcttgat
     5401 gctcaattga gaagaacact tagtccagag atgatcacag tgacttctgc ggttggtcct
     5461 gtgtccatgg cggctccaac agcaatcaca gaagcaggaa cacagcctca gaagggtgtt
     5521 tctcaagtca aagaaggccc tgtcctagca actagttcag gagctggtgt ttttaagatg
     5581 ggacgatttc aggtttctgt tgcagcagac ggtgcccaga aagagggtaa aaataagtca
     5641 gaagatgcaa agtctgttca ttttgaatcc agcacctcag agtcctcagt gctatcaagt
     5701 agtagtccag agagtacctt ggtgaaacca gagccgaatg gcataaccat ccctggtatc
     5761 tcttcagatg tgccagagag tgcccacaaa actactgcct cagaggcaaa gtcagacact
     5821 gggcagccta ccaaggttgg acgttttcag gtgacaacta cagcaaacaa agtgggtcgt
     5881 ttctctgtat caaaaactga ggacaagatc actgacacaa agaaagaagg accagtggca
     5941 tctcctcctt ttatggattt ggaacaagct gttcttcctg ctgtgatacc aaagaaagag
     6001 aagcctgaac tgtcagagcc ttcacatcta aatgggccgt cttctgaccc ggaggccgct
     6061 tttttaagta gggatgtgga tgatggttcc ggtagtccac actcgcccca tcagctgagc
     6121 tcaaagagcc ttcctagcca gaatctaagt caaagcctta gtaattcatt taactcctct
     6181 tacatgagta gcgacaatga gtcagatatc gaagatgaag acttaaagtt agagctgcga
     6241 cgactacgag ataaacatct caaagagatt caggacctgc agagtcgcca gaagcatgaa
     6301 attgaatctt tgtataccaa actgggcaag gtgccccctg ctgttattat tcccccagct
     6361 gctccccttt cagggagaag acgacgaccc actaaaagca aaggcagcaa atctagtcga
     6421 agcagttcct tggggaataa aagcccccag ctttcaggta acctgtctgg tcagagtgca
     6481 gcttcagtct tgcaccccca gcagaccctc caccctcctg gcaacatccc agagtccggg
     6541 cagaatcagc tgttacagcc ccttaagcca tctccctcca gtgacaacct ctattcagcc
     6601 ttcaccagtg atggtgccat ttcagtacca agcctttctg ctccaggtca aggaaccagc
     6661 agcacaaaca ctgttggggc aacagtgaac agccaagccg cccaagctca gcctcctgcc
     6721 atgacgtcca gcaggaaggg cacattcaca gatgacttgc acaagttggt agacaattgg
     6781 gcccgagatg ccatgaatct ctcaggcagg agaggaagca aagggcacat gaattacgag
     6841 ggccctggaa tggcaaggaa gttctctgca cctgggcaac tgtgcatctc catgacctcg
     6901 aacctgggtg gctctgcccc catctctgca gcatcagcta cctctctagg tcacttcacc
     6961 aagtctatgt gccccccaca gcagtatggc tttccagcta ccccatttgg cgctcaatgg
     7021 agtgggacgg gtggcccagc accacagcca cttggccagt tccaacctgt gggaactgcc
     7081 tccttgcaga atttcaacat cagcaatttg cagaaatcca tcagcaaccc cccaggctcc
     7141 aacctgcgga ccacttagta atgaactagt ccgtaacttg aaagtatttc gatttcttgg
     7201 ctttatatat cttgtggaaa ggacgaacca acgagaaaga gttgctgctt
//
