LOCUS       sequence-284718-        3302 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3302)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 3302)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1924
FEATURES             Location/Qualifiers
     source          1..3302
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggagggatt actgcattac atcaaccctg cacacgccat ttctctccta
       61 agtgccctga atgaggagcg tctcaaagga cagctgtgtg atgtgctgct gattgttgga
      121 gaccaaaagt tccgagctca taaaaacgtc ttggctgcca gcagcgaata ctttcagagt
      181 ttattcacaa ataaggaaaa tgagtcacaa actgtatttc agcttgactt ctgtgagcca
      241 gatgcttttg ataatgtttt aaactacatt tattcttcct ctctatttgt tgagaagagc
      301 agccttgctg ctgtgcaaga acttggctat agtcttggga tttcctttct gactaacatc
      361 gtttctaaaa cacctcaagc cccctttcca acgtgtccta atagaaaaaa agtgtttgta
      421 gaagatgatg aaaacagttc tcaaaagaga agtgtcattg tttgtcaaag tagaaacgaa
      481 gcgcaaggaa aaactgttag tcaaaatcaa cccgatgtaa gccatacttc ccggccctct
      541 cctagcattg cagtcaaggc caataccaat aagccacatg tcccaaaacc aatagaacca
      601 cttcataatt tgtcattaac tgaaaagagt tggccgaaag atagttctgt ggtatatgca
      661 aagtctcttg agcattctgg atctttggat gatcctaata gaatcagttt ggtgaaaaga
      721 aatgcagtgt tgccttcaaa gcctctgcaa gacagagaag ctatggatga taaaccaggt
      781 gtgagtggtc agcttccaaa aggaaaagct ctagagctgg ctttgaagag accacggcca
      841 cctgttttgt ctgtttgtag ctcatcagag actccctatc tattaaaaga aactaacaaa
      901 ggaaatggtc aaggtgaaga tagaaacttg ttgtactatt caaagttagg cttagtgatc
      961 ccatccagtg gatctggttc tggaaaccaa agcattgaca ggagtggccc acttgttaag
     1021 agtctcctca gacggtcatt gtcgatggat agccaggttc ctgtctattc accttccata
     1081 gatttgaaat cttcccaggg atcatcttcg gtgtccagtg atgcaccagg gaatgtgttg
     1141 tgtgctttat ctcagaagtc atctttaaaa gattgtagtg aaaaaacagc cctagatgac
     1201 aggcctcaag tgctacaacc gcatcgcctc aggtccttta gtgcttctca gtcaacagac
     1261 agggagggag cttcccctgt gactgaggtg cgcataaaga ctgagcccag cagcccgctg
     1321 tcggacccct cggacatcat ccgcgtcact gtgggagatg cggcaacaac agcagctgcc
     1381 tcatcttcgt cggtcacaag agacctgtct ctgaaaacag aagatgacca aaaagacatg
     1441 agcagactcc cagcaaaaag gaggttccaa gcggaccgaa gattgccgtt taagaagtta
     1501 aaggtgaatg agcacgggtc tcctgtgtca gaagataatt ttgaggaagg ctcaagccct
     1561 actctccttg atgcagattt tccagattct gatttgaata aagacgaatt tggtgagttg
     1621 gaggggacga gaccaaacaa aaaatttaaa tgcaaacatt gccttaagat ctttagatca
     1681 acagcaggtc ttcaccgtca tgttaacatg taccataacc cagaaaagcc ctacgcttgt
     1741 gacatctgtc acaagaggtt tcacaccaac ttcaaagtgt ggacacactg tcagacccaa
     1801 cacggcatag tgaagaaccc atcaccagcc tctagttcac atgctgtttt ggatgaaaaa
     1861 ttccaaagaa agctgattga catagtgaga gagagagaaa ttaagaaggc cctgatcatt
     1921 aagttaaggc gcggcaagcc tggttttcag ggacagagta gctcccaagc acagcaagtc
     1981 atcaagagga acttgagatc tcgagccaaa ggagcttaca tttgtactta ctgcggaaaa
     2041 gcgtaccgct ttctctctca atttaagcag catataaaaa tgcatccagg agaaaaaccc
     2101 cttggagtaa ataaagttgc taaaccaaaa gagcatgctc ctcttgcaag tccagtagaa
     2161 aacaaggagg tttaccagtg ccgcctctgt aatgctaagc tctcttctct cctagagcaa
     2221 ggaagccacg agcggctgtg ccggaacgcg gccgtctgcc cttactgcag cctcaggttt
     2281 ttctcgcccg agctgaagca agaacacgag agcaagtgtg agtataagaa gctgacctgc
     2341 ctcgagtgca tgcgcacctt caagtcctct ttcagcatct ggcggcacca ggttgaagtc
     2401 cataatcaga acaacatggc acccaccgaa aacttttctt tgcccgtttt ggaccacaat
     2461 ggtgatgtga ctggttcttc aaggccccaa tcccagcctg agcccaacaa agtaaaccac
     2521 atcgtcacca caaaagacga caacgtgttc agtgattctt cagaacaagt taacttcgac
     2581 tcggaagatt cctcttgtct tcccgaagac cttagtcttt ccaagcaact gaaaatccaa
     2641 gtcaaagagg agcctgtgga ggaggctgaa gaagaggcac ccgaggccag cacagccccc
     2701 aaagaagcgg gtcctagcaa agaagccagc ctgtggccct gcgagaagtg tgggaagatg
     2761 ttcacggtgc ataagcagct ggagcgtcac caggagcttc tgtgctctgt gaaaccattt
     2821 atttgtcacg tgtgcaacaa agcttttcgc actaattttc gactctggag tcacttccaa
     2881 tcgcacatgt ctcaggcttc agaggaatcg gcacataagg aatctgaggt gtgccctgtt
     2941 cccacaaact ctccctctcc accacctctg ccaccgccac caccactgcc caagatccag
     3001 cctctggagc ctgacagccc cacaggcctg tccgaaaacc caactccagc cacagaaaaa
     3061 ctgtttgtgc cccaagaatc agacaccctt ttttaccatg ccccacccct ttcagcaatc
     3121 acatttaaaa gacagtttat gtgtaaactt tgccacagga cattcaagac tgcatttagt
     3181 ctttggagtc acgaacaaac acacaattag taatgaacta gtccgtaact tgaaagtatt
     3241 tcgatttctt ggctttatat atcttgtgga aaggacgagt cgaaccgtcg aaaggcgagg
     3301 at
//
