LOCUS       sequence-28472-e         731 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Iwakiri Y, Satoh A, Chatterjee S, Toomre DK, Chalouni CM, Fulton D, 
            Groszmann RJ, Shah VH, Sessa WC
  TITLE     Nitric oxide synthase generates nitric oxide locally to regulate 
            compartmentalized protein S-nitrosylation and protein trafficking.
  JOURNAL   Proc Natl Acad Sci U S A. 2006 Dec 26. 103(52):19777-82.
  PUBMED    17170139
REFERENCE   2  (bases 1 to 731)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2006 Dec 26. 103(52):19777-82."
COMMENT     Sequence Label: WT-eNOS-RFP
FEATURES             Location/Qualifiers
     source          1..731
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(23..700)
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     CDS             complement(23..700)
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     primer_bind     complement(85..108)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(283..302)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     552..570
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 ctatagaata gggccctcta gattaggcgc cggtggagtg gcggccctcg gcgcgctcgt
       61 actgttccac gatggtgtag tcctcgttgt gggaggtgat gtccagcttg atgtcggtct
      121 tgtaggcgcc gggcagctgc acgggcttct tggccatgta ggtggtcttg acctcggcgt
      181 cgtagtggcc gccgtccttc agcttcagcc tcatcttgat ctcgcccttc agggcgccgt
      241 cctcggggta catccgctcg gtggaggcct cccagcccat ggtcttcttc tgcattacgg
      301 ggccgtcgga ggggaagttg gtgccgcgca gcttcacctt gtagatgaac tcgccgtcct
      361 gcagggagga gtcctgggtc acggtcacca cgccgccgtc ctcgaagttc atcacacgct
      421 cccacttgaa gccctcgggg aaggacagct tcaagtagtc ggggatgtcg gcggggtgct
      481 tcacgtaggc cttggagccg tactggaact gaggggacag gatgtcccag gcgaagggca
      541 gggggccgcc cttggtcacc ttcagcttgg cggtctgggt gccctcgtag gggcggccct
      601 cgccctcgcc ctcgatctcg aactcgtggc cgttcacgga gccctccatg cgcaccttga
      661 agcgcatgaa ctccttgatg acgtcctcgg aggaggccat gctagcgggg ccgggggtgt
      721 ctgggccggg c
//
