LOCUS       sequence-284721-        1754 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1754)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1754)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1952
FEATURES             Location/Qualifiers
     source          1..1754
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcggaggc ttcttttgga agttcgagcc cagttgggtc tttgtcttct
       61 gaggatcatg attttgaccc cactgctgag atgttggtcc atgactatga tgatgaaaga
      121 actcttgaag aagaggaaat gatggatgag ggtaaaaact tcagttcaga aattgaagac
      181 ttagaaaagg aaggaaccat gcctctagaa gatttactgg cattctatgg ctatgaacct
      241 acaattccag cagttgcaaa ttccagtgca aatagttccc caagtgaact ggcagatgaa
      301 ctaccagaca tgacactaga caaagaggaa atagcaaaag acctgttgtc aggtgatgac
      361 gaggaaactc agtcttctgc ggatgatctg acgccatctg tgacttccca tgaaacttct
      421 gatttcttcc ctaggccttt acgatcaaat actgcatgtg atggtgataa ggaatcagag
      481 gttgaagatg ttgaaacaga cagtggtaat tcacctgaag atttgaggaa ggaaataatg
      541 attggtttac aatatcaggc agagattccc ccttatcttg gagagtacga tggtaatgag
      601 aaagtatatg aaaacgaaga ccagttactt tggtgtcctg atgtggtttt ggagagcaaa
      661 gttaaggaat accttgttga gacttcatta aggactggca gtgaaaaaat aatggatagg
      721 atttctgcag gaacacacac aagggacaat gaacaggcat tatatgaact tctcaagtgt
      781 aaccacaata taaaggaagc aatcgaaaga tactgctgca atggaaaggc ctctcaagaa
      841 ggaatgactg catggacgga agaagaatgc cgaagctttg aacatgcact catgcttttt
      901 ggaaaagatt ttcatcttat acagaagaat aaggtgagaa ctaggacagt tgctgagtgt
      961 gtagcattct actatatgtg gaagaaatct gaacgttatg attactttgc tcaacagaca
     1021 agatttggga aaaaaagata taaccatcac cctggagtta cggactatat ggatcgttta
     1081 gtagatgaaa cagaagcttt gggtgggacg gtaaatgctt cagccttaac ttctaaccgg
     1141 cctgagccta ttcctgatca acagctaaac attctcaact ccttcactgc cagtgacttg
     1201 acagctttga ccaacagtgt agcaaccgtc tgcgacccca cagatgtgaa ttgtttggat
     1261 gatagctttc ctccactggg caacacaccc cgtggacaag ttaatcatgt gcctgttgta
     1321 acagaagagt tactcaccct gcccagcaat ggggaaagtg attgttttaa tttatttgag
     1381 actggatttt atcactcgga gctaaaccct atgaacatgt gcagtgaaga gtcagagaga
     1441 ccagcaaaaa gattgaaaat gggcattgcc gtccctgaat cctttatgaa tgaagtttct
     1501 gtaaataacc tgggtgtgga ctttgaaaat cacacacatc acatcaccag tgccaaaatg
     1561 gctgtttctg tggctgactt tggcagtctc tctgccaacg agaccaatgg tttcatcagt
     1621 gcccatgctc tgcatcagca cgcggcccta cactctgagt agtaatgaac tagtccgtaa
     1681 cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga tgggacacgc
     1741 tatcgctcac tgct
//
