LOCUS       sequence-284726-       13348 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13348)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 13348)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2010
FEATURES             Location/Qualifiers
     source          1..13348
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    9205..9793
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9258..9278)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9676..9687)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9865..10098
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    10176..10297
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10213..10232)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10267..10286
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      10337..10472
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(10492..10511)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10493..10511)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10521..10538)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10521..10537)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10530..10552)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      10679..11134
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10766..10785)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10976..10997
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        11160..11264
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11265..11333,11334..12125)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11483..11502)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      12296..12884
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12785..12804
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     13038..13055
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    13172..13193
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(13208..13213,13214..13231,13232..13238)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13246..13262
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     13251..13273
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     13270..13286
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     13270..13286
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     13305..13325
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        13307..13325
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatgatg gatagtgaga ataaacccga
     4141 aaatgatgag gatgaaaaga taaacaaaga agcacaagac ttgacaaagc tttcatccca
     4201 taatgaagac ggtgggcctg tatctgatgt gatagcaagt ttccctgaga attctatggg
     4261 caaaagaggt ttttcagaat catcgaactc tgatagtgtt gttataggag aagacagaaa
     4321 taaacatgct tccaaacgca ggaaattaga tgaggcagag ccccttaaat ctggaaagca
     4381 aggtatttgt agattagaaa cttctgagag ctcagtcaca gaagggggta ttgcattaga
     4441 tgaaacaggg aaggagacct ttctgagtga ctgcacagtt ggaggcacat gtctcccaaa
     4501 tgccctctcc ccttcttgca attttagcac tattgatgtt gtttctctga aaacagacac
     4561 tgaaaaaaca tctgctcagg aaatggtttc ccttgatctg gaaagagaat ctcctttccc
     4621 cccgaaagaa attagtgtta gttgtaccat tgggaatgta gatacagttc tcaaatgcag
     4681 catctgtggg catttgtttt cttcttgctc tgacttggaa aaacatgctg agtctcacat
     4741 gcagcagcct aaggaacata cctgttgtca ctgcagccac aaagcagaga gcagctcagc
     4801 actacatatg catatcaaac aagcacatgg gccacagaag gtcttttcct gtgatctttg
     4861 tggttttcag tgttcagaag aaaacttgtt gaatgcacat tatcttggca aaacacatct
     4921 ccgtcgtcag aatctggctg ctcgtggagg atttgtacag atcttaacaa aacaaccttt
     4981 tcctaaaaaa tcacgtacaa tggcaacaaa aaatgttcac tcaaaaccaa gaacttctaa
     5041 atcaatagca aagaatagtg attcaaaagg attacgaaat gtgggaagca cgtttaaaga
     5101 tttcagagga agtatttcta aacaaagtgg tagtagcagt gagcttcttg ttgaaatgat
     5161 gccttccaga aatactttgt cacaggaagt agagattgtt gaagaacatg ttacttccct
     5221 tggtctagct cagaatcctg aaaaccagag tagaaagcta gacaccttag taacctcaga
     5281 gggtctctta gagaaattgg aatctacaaa aaataccctt caggcagcac acggtaacag
     5341 tgtaacctcg aggccaagac ctgagcgaaa tattctcgtg ttgggtaata gctttcgtcg
     5401 acgaagcagc actttcacct tgaagggcca ggcaaagaaa aggtttaatc ttttaggaat
     5461 taaaagaggt acaagtgaaa ctcagaggat gtatatgaaa cacttgagaa cacagatgaa
     5521 aacacacgat gcagaatcag tgctgaaaca cctggaagcg tgcagcagtg tgcagagagt
     5581 gtgtgtgact acctcagaaa cccaggaggc agagcagggc caggggagtg cccgtcctcc
     5641 ggactccggg ctgcattccc tgacagtgaa gccagcttct ggctctcaga cgttgtgtgc
     5701 ttgtacagac tgtgggcaag tagctacaaa taggacagat ttggaaatcc atgtgaaaag
     5761 gtgccatgcc agagagatga aattttactg ccgtacttgt gacttctcta gtatgtcaag
     5821 aagggactta gatgaacatt tgcacagtaa ccagcatcag caaactgctt ctgtcctgag
     5881 ttgtcagtgt tgttcattta tatccttgga tgaaataaat cttagagacc acatgaagga
     5941 aaagcacaat atgcattttc tttgcacccc ttgtaatctg ttctttttgt ctgaaaaaga
     6001 tgtggaagaa cacaaagcca ccgagaagca tattaattca ttggttcaac caaagacttt
     6061 gcaatcatct aacagtgatt tggttttaca gactttacct ttgagtactt tagaatcaga
     6121 aaacgcaaaa gagtctatgg atgactcagg aaaagcatct caggaagaac ctctgaagtc
     6181 cagggtaagc catggtaatg aagtgaggca ttccagtaag cctcagtttc agtgtaagaa
     6241 gtgtttttat aaaacaagat cttctactgt tctcacgaga catataaagc ttcggcatgg
     6301 tcaagactat cattttcttt gtaaagcttg taatctttat tcattgagca aagaaggaat
     6361 ggagaaacac attaaaagaa gcaagcatct tgaaaatgct aagaaaaata atattggctt
     6421 aagctttgaa gaatgtattg aaagggtatg tataggtgca aatgataaaa aagaagagtt
     6481 tgatgtttcc ggaaatggaa ggattgaagg ccatataggt gtgcaattac aagagcattc
     6541 ctatcttgag aagggcatgc tggcgtctga ggaactgtca cagtctggtg gtagcaccaa
     6601 agatgatgaa ttagcttcaa ccactactcc aaagagaggg agacctaaag gtaacatctc
     6661 acggacgtgt tcacactgtg gccttttggc ctctagtatt acaaacttga ctgttcacat
     6721 tagacgaaaa cacagtcacc agtatagtta tttatgcaaa gtgtgtaagt attacactgt
     6781 aactaaggga gatatggaac gtcattgtgc caccaagaaa cataaaggac gggtagaaat
     6841 agaagcaagt ggaaaacaca gttcagatat cattgttggc cctgaagggg gtagccttga
     6901 agctggtaaa aagaatgctg gctcagcagt gaccatgtca gatgaacatg ctaacaaacc
     6961 agctgagtca cccacctccg ttttagagaa gccagatcgt ggaaactcaa ttgaagctga
     7021 agttgaaaat gtatttcatt ctctagatgg agaagttaac agccatcttc ttgataaaaa
     7081 ggagcaaata tcttcagagc cagaggactt cgcccagccg ggggatgtgt actcccagag
     7141 agatgttaca ggcacaggtg agaataagtg tttgcactgt gagtttagtg ctcactcctc
     7201 tgcttctcta gagctgcatg taaaacggaa acatacaaaa gagtttgagt tttattgcat
     7261 ggcatgcgat tactacgcgg tgactcgtcg cgagatgacc aggcatgcag caacagagaa
     7321 gcacaaaatg aaaaggcagt cttatctcaa ctctgctaat gtagaagctg gttctgcaga
     7381 catgtccaaa aacatcatta tgcctgaaga agagcatcaa caaaattctg aggaatttca
     7441 aataatttca ggtcaaccat ctgatactct taaatctaga aatgctgcag attgctctat
     7501 tttaaatgag aatactaatt tagatatgtc taaagtgctc tgcgctgctg actctgtaga
     7561 agttgagact gaagaagaat ctaatttcaa tgaagaccat tccttttgtg agactttcca
     7621 acaggctcct gtcaaggata aagttaggaa acctgaggag atgatgtcac ttactatgtc
     7681 ctcaaactat ggctccccaa gcagatttca aaatgaaaat tcaggaagct ctgccttaaa
     7741 ttgtgagaca gcaaagaaaa accatgagat atcgaatgat gcaggtgagc tgcgtgtcca
     7801 ttgtgagggt gaaggaggaa acgcaggaga cggtggaggt gttgtccccc acagacacct
     7861 gtgccctgtg acgctcgatg gggagcgctc ggctgaaagc cctgtgctcg ttgtgacaag
     7921 aataaccaga gaacagggaa atctggagag cgggggtcag aacagagttg cacgtgggca
     7981 tggtttggaa gacttgaaag gtgtccaaga agatcccgtt ctggggaata aggaaattct
     8041 gatgaattca caacatgaaa cagaatttat tttggaggag gatggcccag cttctgatag
     8101 cacagttgaa agtagtgatg tctatgaaac tataattagt attgatgata aagggcaggc
     8161 catgtacagt tttggtcgat ttgactcctc cataataaga ataaagaacc ctgaagatgg
     8221 tgagttgata gaccagtctg aagagggctt gatagcaacg ggagtgagaa ttagtgagct
     8281 gcccttgaaa gactgtgctc aaggtgtgaa aaagaagaaa tctgagggca gttccattgg
     8341 tgagtctaca cgaattcgct gtgatgattg tggcttctta gcagatggac tgagtggact
     8401 gaatgttcac atagccatga agcatcctac aaaagagaag cacttccatt gtttactctg
     8461 tggaaagtcg ttctataccg aaagcaacct tcaccagcat ctggctagtg ccggccacat
     8521 gagaaatgag caggccagtg tggaggagct tccggaggga ggggccacct ttaaatgtgt
     8581 caagtgcaca gagccctttg attctgaaca gaatttattt ttacatatta aaggacagca
     8641 tgaggaattg ctgcgggagg tgaataagta tatagtggaa gacactgagc aaatcaaccg
     8701 cgagagggag gaaaaccagg gaaacgtctg caagtattgt gggaagatgt gtcgaagcag
     8761 caactcgatg gccttcctgg cacacattcg cactcacaca ggatcaaaac cattcaagtg
     8821 caagatatgc cattttgcaa cagctcagct tggagatgcc agaaaccatg tgaaaaggca
     8881 ccttgggatg agggaataca agtgtcatgt ctgtggggtt gcttttgtaa tgaagaagca
     8941 cttaaatact catctactag gcaagcatgg agttggcacc ccaaaagaaa gagtaaaggt
     9001 tgcatatcga aagatcggga cgttgccagg aatacagaac aacaggaacg cgtcgagtgc
     9061 ctctgaagcc caaagtcttt gtgagcactt ttcatagtaa tgaactagtc cgtaacttga
     9121 aagtatttcg atttcttggc tttatatatc ttgtggaaag gacgaatcgc caggagacac
     9181 gtagttcata cgcgttaagt cgacaatcaa cctctggatt acaaaatttg tgaaagattg
     9241 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     9301 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     9361 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     9421 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     9481 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     9541 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa
     9601 tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     9661 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     9721 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     9781 gccgcctccc cgcgtcgact ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
     9841 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc
     9901 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     9961 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
    10021 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
    10081 tgtggaaaat ctctagcagt acgtatagta gttcatgtca tcttattatt cagtatttat
    10141 aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg
    10201 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
    10261 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc
    10321 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
    10381 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
    10441 ccagaagtag tgaggaggct tttttggagg cctagggacg tacccaattc gccctatagt
    10501 gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
    10561 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
    10621 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac
    10681 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
    10741 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
    10801 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
    10861 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
    10921 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
    10981 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
    11041 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
    11101 gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc
    11161 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
    11221 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
    11281 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
    11341 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
    11401 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
    11461 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
    11521 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
    11581 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
    11641 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
    11701 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
    11761 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
    11821 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
    11881 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
    11941 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
    12001 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
    12061 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
    12121 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
    12181 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
    12241 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
    12301 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
    12361 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
    12421 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
    12481 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
    12541 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
    12601 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
    12661 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
    12721 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
    12781 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
    12841 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
    12901 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
    12961 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
    13021 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
    13081 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
    13141 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
    13201 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
    13261 aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt aaccctcact
    13321 aaagggaaca aaagctggag ctgcaagc
//
