LOCUS       sequence-284727-        5084 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5084)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 5084)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2010
FEATURES             Location/Qualifiers
     source          1..5084
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 accgccacca tgatggatag tgagaataaa cccgaaaatg atgaggatga aaagataaac
       61 aaagaagcac aagacttgac aaagctttca tcccataatg aagacggtgg gcctgtatct
      121 gatgtgatag caagtttccc tgagaattct atgggcaaaa gaggtttttc agaatcatcg
      181 aactctgata gtgttgttat aggagaagac agaaataaac atgcttccaa acgcaggaaa
      241 ttagatgagg cagagcccct taaatctgga aagcaaggta tttgtagatt agaaacttct
      301 gagagctcag tcacagaagg gggtattgca ttagatgaaa cagggaagga gacctttctg
      361 agtgactgca cagttggagg cacatgtctc ccaaatgccc tctccccttc ttgcaatttt
      421 agcactattg atgttgtttc tctgaaaaca gacactgaaa aaacatctgc tcaggaaatg
      481 gtttcccttg atctggaaag agaatctcct ttccccccga aagaaattag tgttagttgt
      541 accattggga atgtagatac agttctcaaa tgcagcatct gtgggcattt gttttcttct
      601 tgctctgact tggaaaaaca tgctgagtct cacatgcagc agcctaagga acatacctgt
      661 tgtcactgca gccacaaagc agagagcagc tcagcactac atatgcatat caaacaagca
      721 catgggccac agaaggtctt ttcctgtgat ctttgtggtt ttcagtgttc agaagaaaac
      781 ttgttgaatg cacattatct tggcaaaaca catctccgtc gtcagaatct ggctgctcgt
      841 ggaggatttg tacagatctt aacaaaacaa ccttttccta aaaaatcacg tacaatggca
      901 acaaaaaatg ttcactcaaa accaagaact tctaaatcaa tagcaaagaa tagtgattca
      961 aaaggattac gaaatgtggg aagcacgttt aaagatttca gaggaagtat ttctaaacaa
     1021 agtggtagta gcagtgagct tcttgttgaa atgatgcctt ccagaaatac tttgtcacag
     1081 gaagtagaga ttgttgaaga acatgttact tcccttggtc tagctcagaa tcctgaaaac
     1141 cagagtagaa agctagacac cttagtaacc tcagagggtc tcttagagaa attggaatct
     1201 acaaaaaata cccttcaggc agcacacggt aacagtgtaa cctcgaggcc aagacctgag
     1261 cgaaatattc tcgtgttggg taatagcttt cgtcgacgaa gcagcacttt caccttgaag
     1321 ggccaggcaa agaaaaggtt taatctttta ggaattaaaa gaggtacaag tgaaactcag
     1381 aggatgtata tgaaacactt gagaacacag atgaaaacac acgatgcaga atcagtgctg
     1441 aaacacctgg aagcgtgcag cagtgtgcag agagtgtgtg tgactacctc agaaacccag
     1501 gaggcagagc agggccaggg gagtgcccgt cctccggact ccgggctgca ttccctgaca
     1561 gtgaagccag cttctggctc tcagacgttg tgtgcttgta cagactgtgg gcaagtagct
     1621 acaaatagga cagatttgga aatccatgtg aaaaggtgcc atgccagaga gatgaaattt
     1681 tactgccgta cttgtgactt ctctagtatg tcaagaaggg acttagatga acatttgcac
     1741 agtaaccagc atcagcaaac tgcttctgtc ctgagttgtc agtgttgttc atttatatcc
     1801 ttggatgaaa taaatcttag agaccacatg aaggaaaagc acaatatgca ttttctttgc
     1861 accccttgta atctgttctt tttgtctgaa aaagatgtgg aagaacacaa agccaccgag
     1921 aagcatatta attcattggt tcaaccaaag actttgcaat catctaacag tgatttggtt
     1981 ttacagactt tacctttgag tactttagaa tcagaaaacg caaaagagtc tatggatgac
     2041 tcaggaaaag catctcagga agaacctctg aagtccaggg taagccatgg taatgaagtg
     2101 aggcattcca gtaagcctca gtttcagtgt aagaagtgtt tttataaaac aagatcttct
     2161 actgttctca cgagacatat aaagcttcgg catggtcaag actatcattt tctttgtaaa
     2221 gcttgtaatc tttattcatt gagcaaagaa ggaatggaga aacacattaa aagaagcaag
     2281 catcttgaaa atgctaagaa aaataatatt ggcttaagct ttgaagaatg tattgaaagg
     2341 gtatgtatag gtgcaaatga taaaaaagaa gagtttgatg tttccggaaa tggaaggatt
     2401 gaaggccata taggtgtgca attacaagag cattcctatc ttgagaaggg catgctggcg
     2461 tctgaggaac tgtcacagtc tggtggtagc accaaagatg atgaattagc ttcaaccact
     2521 actccaaaga gagggagacc taaaggtaac atctcacgga cgtgttcaca ctgtggcctt
     2581 ttggcctcta gtattacaaa cttgactgtt cacattagac gaaaacacag tcaccagtat
     2641 agttatttat gcaaagtgtg taagtattac actgtaacta agggagatat ggaacgtcat
     2701 tgtgccacca agaaacataa aggacgggta gaaatagaag caagtggaaa acacagttca
     2761 gatatcattg ttggccctga agggggtagc cttgaagctg gtaaaaagaa tgctggctca
     2821 gcagtgacca tgtcagatga acatgctaac aaaccagctg agtcacccac ctccgtttta
     2881 gagaagccag atcgtggaaa ctcaattgaa gctgaagttg aaaatgtatt tcattctcta
     2941 gatggagaag ttaacagcca tcttcttgat aaaaaggagc aaatatcttc agagccagag
     3001 gacttcgccc agccggggga tgtgtactcc cagagagatg ttacaggcac aggtgagaat
     3061 aagtgtttgc actgtgagtt tagtgctcac tcctctgctt ctctagagct gcatgtaaaa
     3121 cggaaacata caaaagagtt tgagttttat tgcatggcat gcgattacta cgcggtgact
     3181 cgtcgcgaga tgaccaggca tgcagcaaca gagaagcaca aaatgaaaag gcagtcttat
     3241 ctcaactctg ctaatgtaga agctggttct gcagacatgt ccaaaaacat cattatgcct
     3301 gaagaagagc atcaacaaaa ttctgaggaa tttcaaataa tttcaggtca accatctgat
     3361 actcttaaat ctagaaatgc tgcagattgc tctattttaa atgagaatac taatttagat
     3421 atgtctaaag tgctctgcgc tgctgactct gtagaagttg agactgaaga agaatctaat
     3481 ttcaatgaag accattcctt ttgtgagact ttccaacagg ctcctgtcaa ggataaagtt
     3541 aggaaacctg aggagatgat gtcacttact atgtcctcaa actatggctc cccaagcaga
     3601 tttcaaaatg aaaattcagg aagctctgcc ttaaattgtg agacagcaaa gaaaaaccat
     3661 gagatatcga atgatgcagg tgagctgcgt gtccattgtg agggtgaagg aggaaacgca
     3721 ggagacggtg gaggtgttgt cccccacaga cacctgtgcc ctgtgacgct cgatggggag
     3781 cgctcggctg aaagccctgt gctcgttgtg acaagaataa ccagagaaca gggaaatctg
     3841 gagagcgggg gtcagaacag agttgcacgt gggcatggtt tggaagactt gaaaggtgtc
     3901 caagaagatc ccgttctggg gaataaggaa attctgatga attcacaaca tgaaacagaa
     3961 tttattttgg aggaggatgg cccagcttct gatagcacag ttgaaagtag tgatgtctat
     4021 gaaactataa ttagtattga tgataaaggg caggccatgt acagttttgg tcgatttgac
     4081 tcctccataa taagaataaa gaaccctgaa gatggtgagt tgatagacca gtctgaagag
     4141 ggcttgatag caacgggagt gagaattagt gagctgccct tgaaagactg tgctcaaggt
     4201 gtgaaaaaga agaaatctga gggcagttcc attggtgagt ctacacgaat tcgctgtgat
     4261 gattgtggct tcttagcaga tggactgagt ggactgaatg ttcacatagc catgaagcat
     4321 cctacaaaag agaagcactt ccattgttta ctctgtggaa agtcgttcta taccgaaagc
     4381 aaccttcacc agcatctggc tagtgccggc cacatgagaa atgagcaggc cagtgtggag
     4441 gagcttccgg agggaggggc cacctttaaa tgtgtcaagt gcacagagcc ctttgattct
     4501 gaacagaatt tatttttaca tattaaagga cagcatgagg aattgctgcg ggaggtgaat
     4561 aagtatatag tggaagacac tgagcaaatc aaccgcgaga gggaggaaaa ccagggaaac
     4621 gtctgcaagt attgtgggaa gatgtgtcga agcagcaact cgatggcctt cctggcacac
     4681 attcgcactc acacaggatc aaaaccattc aagtgcaaga tatgccattt tgcaacagct
     4741 cagcttggag atgccagaaa ccatgtgaaa aggcaccttg ggatgaggga atacaagtgt
     4801 catgtctgtg gggttgcttt tgtaatgaag aagcacttaa atactcatct actaggcaag
     4861 catggagttg gcaccccaaa agaaagagta aaggttgcat atcgaaagat cgggacgttg
     4921 ccaggaatac agaacaacag gaacgcgtcg agtgcctctg aagcccaaag tctttgtgag
     4981 cacttttcat agtaatgaac tagtccgtaa cttgaaagta tttcgatttc ttggctttat
     5041 atatcttgtg gaaaggacga atcgccagga gacacgtagt tcat
//
