LOCUS       sequence-28474-e         711 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 711)
  AUTHORS   Lovas G, Li W, Pott U, Verga T, Hudson LD
  TITLE     Expression of the Kruppel-type zinc finger protein rKr2 in the 
            developing nervous system.
  JOURNAL   Glia. 2001 Apr 15. 34(2):110-20.
  PUBMED    11307160
REFERENCE   2  (bases 1 to 711)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Glia. 2001 
            Apr 15. 34(2):110-20."
COMMENT     Sequence Label: pHisrKr2
FEATURES             Location/Qualifiers
     source          1..711
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             56..85
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             98..109
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(670..688)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 tgcganattc cctctgaata ttttgtttac tttaagaagg agatatacca tgggccatca
       61 tcatcatcat catcatcatc atcacagcag cggccatatc gaaggtcgtc atatgctcga
      121 gggaggccag agtccaaata agatgaagac tgttgatgca acaggagtga ggtgcctttt
      181 actggggcag cttctgtact gggaaataac aggccacgat gccaacaaac tggccagagc
      241 tccggaagct gtagtccaca ctcaaggaaa gggttctcac ttcctggagc aaggccactc
      301 ctcctgccac agggaagcag aggagcctcg ccggccttct gaggatgaca gttctctcga
      361 cagtctgaca cgtgaccatt ccagcactac tgaaaatcag gaatttctgt ctgggagagc
      421 ccaggggtct tggtgtaaaa cacatcttag gaaaagacag aaccatcaga aacactgtct
      481 gcagactctg atgaaaaaca agccagagct gttgactcag cattcccctg tacaaagaac
      541 acaggaggac agcaggcata gctctagcgt ccccattcaa caaagtgttc atccaggaag
      601 aaagcgctac tggtctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg
      661 ctgctgccac cgctgagcaa taactagcat aaccccttgg gcnacntaac g
//
