LOCUS       sequence-284742-        1178 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1178)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1178)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2546
FEATURES             Location/Qualifiers
     source          1..1178
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggagtcggc cgacttctac gaggcggagc cgcggccccc gatgagcagc
       61 cacctgcaga gccccccgca cgcgcccagc agcgccgcct tcggctttcc ccggggcgcg
      121 ggccccgcgc agcctcccgc cccacctgcc gccccggagc cgctgggcgg catctgcgag
      181 cacgagacgt ccatcgacat cagcgcctac atcgacccgg ccgccttcaa cgacgagttc
      241 ctggccgacc tgttccagca cagccggcag caggagaagg ccaaggcggc cgtgggcccc
      301 acgggcggcg gcggcggcgg cgactttgac tacccgggcg cgcccgcggg ccccggcggc
      361 gccgtcatgc ccgggggagc gcacgggccc ccgcccggct acggctgcgc ggccgccggc
      421 tacctggacg gcaggctgga gcccctgtac gagcgcgtcg gggcgccggc gctgcggccg
      481 ctggtgatca agcaggagcc ccgcgaggag gatgaagcca agcagctggc gctggccggc
      541 ctcttccctt accagccgcc gccgccgccg ccgccctcgc acccgcaccc gcacccgccg
      601 cccgcgcacc tggccgcccc gcacctgcag ttccagatcg cgcactgcgg ccagaccacc
      661 atgcacctgc agcccggtca ccccacgccg ccgcccacgc ccgtgcccag cccgcacccc
      721 gcgcccgcgc tcggtgccgc cggcctgccg ggccctggca gcgcgctcaa ggggctgggc
      781 gccgcgcacc ccgacctccg cgcgagtggc ggcagcggcg cgggcaaggc caagaagtcg
      841 gtggacaaga acagcaacga gtaccgggtg cggcgcgagc gcaacaacat cgcggtgcgc
      901 aagagccgcg acaaggccaa gcagcgcaac gtggagacgc agcagaaggt gctggagctg
      961 accagtgaca atgaccgcct gcgcaagcgg gtggaacagc tgagccgcga actggacacg
     1021 ctgcggggca tcttccgcca gctgccagag agctccttgg tcaaggccat gggcaactgc
     1081 gcgtagtaat gaactagtcc gtaacttgaa agtatttcga tttcttggct ttatatatct
     1141 tgtggaaagg acgatcgaat ttagggctct gtccttcc
//
