LOCUS       sequence-284748-        1766 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1766)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1766)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2591
FEATURES             Location/Qualifiers
     source          1..1766
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggctgtgga tctgctgtct gctcaggagc ctgtgacatt cagggatgtg
       61 gccgtgttct tcagccagga cgagtggttg cacctggact ctgcccagag ggccttgtac
      121 cgggaggtga tgctggagaa ctacagcagc ctggtctcac tggggattcc attttcaatg
      181 ccaaagttga ttcatcagtt gcagcaagga gaagatccct gcatggtgga aagagaagtc
      241 ccttcagata cccgtctagg tttcaagact tggcttgaaa cagaagcatt gcctcataga
      301 caggacattt ttatagaaga aacatctcag ggaatggtaa agaaagaatc cattaaggat
      361 ggtcactggg acattaactt tgaagaagct gtggaatttg agagcgagat agaagaagag
      421 caagagaaga aacctcttag acaaatgata gattcgcatg agaaaaccat cagtgaagat
      481 ggaaaccata caagtcttga attggggaaa agcttattta caaatacagc tcttgtcaca
      541 caacagagtg ttcctataga aaggataccc aatatgtatt atacatttgg gaaagatttt
      601 aaacagaatt ttgatctcat gaaatgcttc cagatttacc caggaggaaa acctcacatc
      661 tgtaatgaat gtgggaagag cttcaagcag aatctgcatc ttattgaaca tcagagaatt
      721 catacaggtg agaaacccta caaatgtaat gagtgtgaaa aaaccttcag ccacagatca
      781 tcccttcttt ctcatcagag aattcatact ggagagaaac cttacaagtg taatgaatgt
      841 gagaaggcat ttagcaacag ttcaaccctt atcaaacatc tgagagtgca tactggagag
      901 aaaccgtatc gatgtaggga atgtggtaaa gcctttagcc agtgttcaac cctcactgta
      961 catcagagaa ttcatactgg agagaaactc tataaatgcg gcgaatgtga gaaggccttc
     1021 aactgtagag caaaacttca caggcatcaa agaatccata caggtgagaa accctataaa
     1081 tgtagtgagt gtgggaaggg atacagccag tttacatctc tagctgaaca tcagaggttt
     1141 catactggag aacaactgta tacatgcttg gaatgtggga gaaccttcac acgtattgta
     1201 acccttatcg aacatcagcg aattcacact ggacaaaaac cttatcagtg caacgaatgt
     1261 gagaaagcct tcaaccagta ttcatccttt aatgaacatc ggaaaattca tactggggaa
     1321 aaactttata catgtgagga atgtgggaaa gcctttggtt gcaaatctaa cctttatagg
     1381 catcagagaa ttcatactgg agagaaaccg tatcagtgta atcagtgtgg aaaggccttc
     1441 agccagtatt catttttaac cgaacatgag aggatccaca ctggagagaa actgtataaa
     1501 tgtatggaat gtgggaaagc ctacagttac agatcaaacc tttgtagaca caaaaaagtt
     1561 cacacgaaag agaaactcta taagtggaag gaatatggga aacctttcat ctgcagctcc
     1621 tcacttaccc agtatcagag attttttaaa ggagataaag cctatgaggt ttagtaatga
     1681 actagtccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac
     1741 gagtgacaga aaccgaatgc gctttg
//
