LOCUS       sequence-28475-e         950 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 950)
  TITLE     MSCV P2Gm +SwaI
REFERENCE   2  (bases 1 to 950)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: MSCV P2Gm +SwaI
FEATURES             Location/Qualifiers
     source          1..950
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     ncRNA           87..208
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           235..249
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           277..403
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     primer_bind     complement(513..533)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 gagctgtacn agtagcggcc gcagcagcag catttaaatg gccgcaagcc ttgttaagtg
       61 ctcgcttcgg cagcacatat actatgttga atgaggcttc agtactttac agaatcgttg
      121 cctgcacatc ttggaaacac ttgctgggat tacttcttca ggttaaccca acagaaggct
      181 cgagaaggta tattgctgtt gacagtgagc gagctcccgt gaattggaat cctagtgaag
      241 ccacagatgt aggattccaa ttcagcggga gcctgcctac tgcctcggaa ttcaaggggc
      301 tactttagga gcaattatct tgtttactaa aactgaatac cttgctatct ctttgataca
      361 tttttacaaa gctgaattaa aatggtataa attaaatcac ttttttcaat tggaagacta
      421 atgcgtttaa actgtcgacc tgcagccaag cttatcgata atcaacctct ggattacaaa
      481 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
      541 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
      601 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
      661 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
      721 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
      781 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgng
      841 gngttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt
      901 ctgcgcggga ngnccttctg ctacgtccct tcggccntca atccagcgga
//
