LOCUS       sequence-284784-        1226 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1226)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1226)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2727
FEATURES             Location/Qualifiers
     source          1..1226
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggatgtgat gctggagaat tatagcaact tactttcagt ggaagtgtgg
       61 aaggctgatg accagatgga gagagaccac agaaacccag acgagcaggc gaggcaattt
      121 ttaattctta agaaccaaac cccaattgag gaaagaggcg atctctttgg aaaagcactt
      181 aatctgaaca cagactttgt ttctttaaga caagtacctt ataaatatga cttatatgaa
      241 aaaactttga aatataattc agacttgctt aatagtaata gaagctatgc aggaaagcag
      301 actgatgagt gtaatgaatt tggaaaagca cttctctacc tgaagcaaga gaaaacccac
      361 agtggagtag aatattctga atacaataaa agtggaaaag ccctcagcca taaagcagcc
      421 atttttaaac atcagaaaat aaaaaacttg gttcaacctt tcatttgtac ttactgtgac
      481 aaggctttct cctttaagtc actcctcatt agtcataaga gaatacatac tggagaaaag
      541 ccatatgaat gcaatgtatg taagaaaacc ttctcccata aggccaacct catcaaacat
      601 cagagaattc acactgggga gaaacctttc gagtgtccgg aatgtggaaa agctttcacc
      661 caccagtcaa acctcattgt acaccagaga gcacatatgg agaagaagcc ctatgagtgc
      721 agtgaatgtg gaaagacatt tgcccaaaag tttgaactca ccacacacca gagaattcat
      781 acaggagagc gaccctatga gtgtaacgaa tgtgcaaaaa ccttctttaa gaagtcaaac
      841 cttatcatac atcagaagat tcacacgggg gagaaacgct atgagtgcag tgaatgtgga
      901 aaatccttta tccagaactc acagctcatc atacacatga gaactcatac aggagagaaa
      961 ccctatgaat gtactgagtg cggcaaaact ttcagccaga ggtcaactct tagattacac
     1021 ttgcgaatcc acacaggaga gaaaccatat gagtgttccg aatgtgggaa ggcctttagc
     1081 aggaagtccc gactcagtgt ccatcagaga gttcacatcg gggagaaacc ctagtaatga
     1141 actagtccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac
     1201 gaagcatcct tgactactgt ctcggg
//
