LOCUS       sequence-284814-        4844 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4844)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 4844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2772
FEATURES             Location/Qualifiers
     source          1..4844
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             340..351
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 accgccacca tggaggcggc caccacactg cacccaggcc cgcgcccggc gctgcccctc
       61 gggggcccgg gcccgctggg cgagttcctg cctccacccg agtgcccggt cttcgaaccc
      121 agctgggaag agttcgcgga ccccttcgct ttcatccaca agatccggcc catagccgag
      181 cagactggca tctgtaaggt gcggccgccg ccggattggc agccaccatt tgcatgtgat
      241 gttgataaac ttcattttac gccacgtatc cagagactga atgaattgga ggcccaaact
      301 cgtgtaaaat tgaatttctt ggaccagatt gcaaagtact gggagttaca gggaagtact
      361 ctgaaaattc cacatgtgga gaggaagatc ttggacttat ttcagcttaa taagttagtt
      421 gcagaagaag gtggatttgc agttgtttgc aaggatagaa aatggaccaa aattgctacc
      481 aagatggggt ttgctcctgg caaagcagtg ggctcacata tcagagggca ttatgaacga
      541 attctcaacc cctacaactt attcctgtcc ggagacagcc taaggtgttt gcagaagcca
      601 aacctgacca cagacactaa ggacaaggag tacaaacccc atgatattcc ccagaggcag
      661 tctgtgcagc cttcggaaac gtgcccccca gcccgacgag caaaacgcat gagagcagag
      721 agacagagtc tcgctgtgtt gcccaggctg gagtgcagtg gcgcgatctt ggctcactgc
      781 aacctccgcc tcctagattc cagcaattct tctgcctcag cctcccaagc catgaatatt
      841 aaaatagaac ccgaggagac aacggaagcc agaactcata atctgagacg tcgaatgggt
      901 tgtccaactc caaaatgtga aaatgagaaa gaaatgaaga gtagcatcaa gcaagaacct
      961 attgagagga aagattatat tgtagaaaat gagaaggaaa agcccaagag tcgatctaaa
     1021 aaagccacca atgctgtgga cctgtatgtc tgtcttttat gtggcagtgg caatgatgaa
     1081 gaccggctac tgttgtgtga tggctgtgat gacagttacc ataccttttg cttgatccca
     1141 cctctccatg atgttcccaa gggagactgg aggtgtccta agtgtttggc tcaggaatgt
     1201 agtaagccac aagaagcatt tggctttgaa caagcagcca gggactatac cctccgtact
     1261 tttggggaaa tggcagatgc gttcaaatct gattacttca acatgccagt ccatatggtc
     1321 cccacagagc ttgttgagaa agaattttgg agactagtaa gcactattga ggaggatgtc
     1381 acagtggaat atggagctga cattgcctca aaggaatttg gcagtggctt tcctgtccga
     1441 gatgggaaaa tcaaactctc acctgaggaa gaggagtatc ttgatagtgg ctggaatttg
     1501 aacaacatgc cagtgatgga gcagtctgtc cttgcacata ttactgctga tatatgtggc
     1561 atgaaacttc cttggttgta tgtgggaatg tgcttttctt cattctgttg gcacattgaa
     1621 gaccactgga gctattcaat taactacttg cactggggtg agccaaaaac ctggtatgga
     1681 gtcccagggt atgctgctga gcagctagaa aatgtaatga agaaactagc tccagaactc
     1741 tttgtgtccc agccggatct cctccatcag cttgtgacca tcatgaaccc caataccctg
     1801 atgactcatg aagtgcctgt ttaccgaact aatcagtgtg ctggggagtt tgtgattaca
     1861 tttccaagag cctaccacag tggttttaac cagggtttta attttgctga ggctgttaac
     1921 ttctgcactg ttgattggct gccattaggc cgacagtgtg tggagcatta tcgcttgctt
     1981 catcgatatt gtgtgttttc ccacgatgag atgatctgca agatggcttc caaggctgat
     2041 gtattagatg ttgtagtggc ttcaactgtt cagaaagaca tggccattat gattgaggat
     2101 gagaaagctt taagagaaac tgtccgtaaa ttgggagtga ttgattcgga aagaatggat
     2161 tttgagctgt tgccagatga tgaacgtcag tgtgtaaaat gcaaaactac atgcttcatg
     2221 tctgccatct cctgttcttg taaacctggc cttcttgttt gcctgcatca tgtaaaagaa
     2281 ttgtgttcct gtcctcctta caaatataaa ttgcggtata ggtacacgct ggatgatctc
     2341 taccctatga tgaatgcatt gaagcttcga gcagaatctt acaacgaatg ggccttgaat
     2401 gtgaatgaag ctttggaggc aaagatcaac aagaagaaaa gccttgtcag cttcaaggct
     2461 ttaattgaag aatctgaaat gaagaaattc ccagacaatg atcttttgcg acaccttcgc
     2521 ctagtcacac aggatgcaga gaagtgtgcc tctgttgcgc agcagttgct taatggcaaa
     2581 aggcaaacta gatatcgatc tggtggaggg aaatcccaaa atcagttgac agtgaatgag
     2641 ctccggcagt ttgtaacaca gctgtatgct cttccatgtg tcctcagtca gacaccatta
     2701 ctaaaggatc tcttgaatcg tgtagaagat tttcaacagc atagtcagaa actactctct
     2761 gaggaaacgc ctagtgctgc ggagctgcag gacttgctag atgtcagctt tgaatttgat
     2821 gttgaacttc cacagcttgc tgagatgcgt atccgtttgg aacaagcccg ttggctagaa
     2881 gaggtgcagc aagcttgcct agaccccagc tcccttactt tagatgatat gagacgtctc
     2941 atagacctag gggtagggct ggccccgtat tcagcagtgg agaaagctat ggcccggctg
     3001 caggaactgc tcacagtgtc agagcactgg gacgacaaag ccaagagtct cctcaaggcc
     3061 aggccacgac attcattgaa tagccttgct acggcagtaa aggaaatcga agagatccct
     3121 gcatatctgc ccaatggtgc ggctctgaaa gactcagtgc agagagccag agactggctt
     3181 caggatgtag agggcctgca ggctggagga cgtgtgccag tgttagacac actcatagaa
     3241 cttgttacac gaggccgatc tatccccgta catctgaatt ctttgccaag actggaaacc
     3301 ctagtagctg aggttcaggc ttggaaagaa tgtgctgtta atacattctt gactgagaat
     3361 tctccatatt ctctcttaga ggtgctgtgt cctcgatgtg atattggcct tttgggattg
     3421 aaaaggaagc agagaaagtt aaaggagccc ttgccaaatg gaaagaaaaa aagcaccaaa
     3481 ttagagagtc tgagtgacct ggagagagct ttaactgaaa gcaaggagac tgcttcagct
     3541 atggcaactc ttggggaagc tcgcctaagg gaaatggaag ccttgcagtc tctcagactc
     3601 gccaatgaag ggaaattgct gtcgcctctc caagatgtgg atataaaaat ctgcctatgt
     3661 cagaaggccc cagctgcccc tatgattcaa tgtgaactct gcagggatgc tttccacacc
     3721 agttgtgtgg cggtacccag tatttcacag ggcctgcgaa tctggctttg tccccattgt
     3781 cggaggtcag agaaacctcc attagagaaa attctgcccc tgctcgcctc ccttcagcgt
     3841 atccgagttc gccttcctga gggagatgca cttcgatata tgattgaaag aaccgtgaac
     3901 tggcagcaca gagcccagca actgctttcg tcagggaatc ttaaatttgt gcaagatcga
     3961 gtgggctcag gactgttata tagcagatgg caagcctcag caggacaggt gtcagacaca
     4021 aacaaggtat ctcaacctcc tggcacaaca tcattttctt tgcctgatga ctgggacaac
     4081 agaacctcat atttgcactc ccccttctca actggacgaa gttgtatccc cctccatggt
     4141 gttagtccag aagtgaatga actattgatg gaagcccagc tgctccaggt atcccttcct
     4201 gaaattcagg aactttacca gactttactt gcaaagccaa gccctgctca gcagactgac
     4261 cgaagctcac cagtgagacc cagcagtgag aagaatgact gttgccgagg gaagcgagat
     4321 ggaattaaca gtcttgagag aaaactgaag agacgcctgg aaagagaggg cctctccagt
     4381 gagcggtggg aacgagttaa gaaaatgcgg acccccaaaa agaagaaaat caaactgagc
     4441 caccccaagg acatgaacaa tttcaagtta gagagagagc gtagctatga attagttcgt
     4501 tctgctgaaa ctcattccct gccctcagac acatcctatt ccgaacagga agactctgag
     4561 gatgaagatg ccatctgccc agctgtgagc tgcctgcagc cagaaggaga tgaggtggac
     4621 tgggtccagt gtgatggcag ctgcaatcag tggtttcatc aggtctgtgt tggtgtctcc
     4681 ccagagatgg cagagaaaga agactacatc tgtgtgcgct gtactgtgaa ggacgcacca
     4741 agccgaaagt agtaatgaac tagtccgtaa cttgaaagta tttcgatttc ttggctttat
     4801 atatcttgtg gaaaggacga gcacgtatac gataacactg ccga
//
