LOCUS       sequence-284820-        4781 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4781)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 4781)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2776
FEATURES             Location/Qualifiers
     source          1..4781
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    1006..1017
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
ORIGIN
        1 accgccacca tggagccggg gtccgacgat ttcctaccgc caccggagtg cccggtgttc
       61 gagcctagct gggccgagtt ccgagaccct cttggctaca tcgcgaaaat caggcccatc
      121 gcagagaaat cgggcatttg caagatccgc ccacccgcgg actggcagcc accctttgct
      181 gtggaagtgg acaacttcag gtttaccccc cgaatccaga ggctgaatga gctagaggcc
      241 cagacgagag tgaaactgaa ctacttggac cagattgcca aattctggga aatccagggc
      301 tcctccttaa agattcccaa tgtagaacgg cggatcttgg acctctacag tctcagcaaa
      361 attgtggtgg aggaaggtgg ttatgaagct atctgcaagg accgtcggtg ggctcgggta
      421 gcccagcgcc tcaactatcc accaggcaaa aatattggct ccttgctacg ctcccactac
      481 gaacgcattg tttatcccta tgaaatgtac cagtctggag ccaaccttgt gtgtaacaca
      541 cgtccatttg ataatgagga gaaggacaag gaatacaaac cccacagcat ccccctacga
      601 cagtctgtgc agccttccaa gttcaacagc tatggccggc gggccaagag actgcagcct
      661 gatccggaac ccacagagga agacattgag aagaatccag agctgaaaaa gctacagatc
      721 tatggggcag gccccaagat gatgggcctg ggcctcatgg ccaaagacaa gactctgcgg
      781 aagaaagata aggaggggcc tgagtgtccc cccacagtag tggtgaagga ggagttaggt
      841 ggggatgtga aggtggagtc aacatcgcct aagaccttcc tggagagcaa ggaggagctg
      901 agtcacagcc cagaaccctg caccaagatg accatgaggc tacggaggaa ccacagcaat
      961 gcccagttta ttgagtcata tgtctgccgg atgtgttctc gaggggatga ggatgacaag
     1021 ctcctgctgt gtgatggctg tgatgacaac taccacatct tctgcctgct gcctcctctg
     1081 cctgagatcc ccaagggtgt ctggcggtgc ccaaagtgtg tcatggcgga gtgtaagcgg
     1141 cccccagaag cctttggctt tgagcaggct acccgggaat acactctgca gagctttggc
     1201 gagatggccg actcctttaa agctgactac ttcaacatgc ccgtgcatat ggtgcccaca
     1261 gaacttgtgg agaaggagtt ctggaggctg gtaaatagca ttgaggaaga tgtgactgtt
     1321 gagtatggag ctgacatcca ttccaaagaa tttggcagcg gtttccctgt cagtgacagt
     1381 aaacggcacc taacccccga agaggaggag tatgctacca gtggttggaa cctaaatgtg
     1441 atgccggtgt tggaacagtc tgtactgtgc cacatcaatg cagatatctc tggcatgaag
     1501 gtgccctggc tctacgtggg catggtcttc tcagcctttt gctggcatat tgaggatcac
     1561 tggagttact ccattaacta cctccactgg ggtgagccga agacctggta tggggtgccc
     1621 tcacttgcag cagaacattt ggaagaagtg atgaagaagc tgacacctga actatttgat
     1681 agccagcctg acctcctgca ccaacttgtc accctcatga atcccaacac cctcatgtcc
     1741 catggtgtgc cagttgtccg cacaaaccag tgtgcaggag agtttgtcat caccttcccc
     1801 cgtgcttacc acagcggctt caaccaaggc tacaactttg ccgaggctgt caacttttgc
     1861 actgctgact ggttgcctgc tgggcgccag tgcattgagc actaccgccg gctccggaga
     1921 tactgcgtct tctcccatga ggagcttatc tgcaagatgg ctgcctgccc agagaagcta
     1981 gacctgaacc tggcggcagc tgtgcataag gagatgttca tcatggtgca agaagagcgg
     2041 cgtctacgaa aggccctgct ggagaagggt atcacagagg ctgagcgaga ggctttcgag
     2101 ctgctcccag atgatgagcg ccagtgtatc aagtgcaaga ctacgtgttt cctgtcagcc
     2161 ctggcctgct acgactgccc agacggcctt gtctgccttt cccacatcaa tgatctctgc
     2221 aagtgctcca gtagccggca gtacctgcgg tatcggtata ccttggatga gcttcctgcc
     2281 atgctgcata agctgaaggt tcgggctgag tcctttgaca cctgggccaa caaagtgcga
     2341 gtggccctgg aggtggagga tgggcggaag cgcagccttg aagaactgag ggcactagag
     2401 tctgaagccc gtgagcggag gtttcctaat agtgagctgc tgcagcaact aaagaactgc
     2461 ctgagtgagg cagaggcttg cgtgtcccga gctctgggac tggtcagcgg ccaggaagct
     2521 ggcccccaca gggtggctgg tctacagatg accctgactg agctccgggc ctttctggac
     2581 cagatgaaca acctgccttg cgccatgcac cagattgggg atgtcaaggg tgttctggaa
     2641 caggtggagg cctaccaggc tgaggctcgt gaggccctgg cctcactgcc ctccagtcca
     2701 gggctactgc agtccctgtt ggagaggggg cggcagctgg gggtggaggt gcctgaggcc
     2761 cagcagctcc agcggcaggt ggaacaggcg cgatggctgg atgaggtgaa acgcacactg
     2821 gccccctcag cccgaagggg caccttggct gtcatgcgag gactgttggt cgcgggtgcc
     2881 agtgtagccc ctagccctgc tgtggataaa gcccaggccg agctgcagga actgctgacc
     2941 attgctgaac gctgggagga gaaagcccac ctctgcctgg aggccaggca gaagcatcca
     3001 ccagccacac ttgaggccat aatccgtgaa gcggaaaaca tccctgttca cctgcccaac
     3061 atccaggctc tcaaggaggc tcttgctaag gcccgggcct ggattgctga tgttgatgag
     3121 atccaaaatg gtgaccacta cccctgcctg gatgacttgg agggcctagt agctgtgggc
     3181 cgggacctac ctgtggggct ggaggagctg agacagctag agctacaggt actgacagcg
     3241 cactcctgga gggagaaggc ctccaagacc ttcctcaaga aaaattcttg ctacacgctg
     3301 ctggaggttc tctgcccatg tgcagatgcc ggctcagaca gcaccaagcg cagccggtgg
     3361 atggagaagg agctggggtt gtacaaatct gacacagagc tgctggggct gtctgcgcag
     3421 gacctcaggg acccaggctc tgtgatcgtg gccttcaagg agggggaaca gaaggagaag
     3481 gagggtatcc tgcagctgcg tcgcaccaat tcggccaagc ccagtccact ggcatcatcg
     3541 agcacggcct cctctacaac ctctatctgt gtgtgtgggc aggtgctggc tggggcggga
     3601 gctctgcagt gtgacctgtg tcaggactgg ttccatgggc ggtgtgtgtc agtgcctcgc
     3661 ctcctcagct ctccgaggcc caatcccacc tcatccccac tgctggcctg gtgggaatgg
     3721 gacaccaaat tcctgtgtcc actgtgtatg cgctcaaggc gcccgcgcct ggagaccatc
     3781 ctggcactgc tggtagccct gcagagactg cctgtgcggc tgcccgaggg cgaggccctg
     3841 cagtgcctca cagagagggc catcagctgg caaggccgcg ccaggcaggc tctggcctct
     3901 gaagatgtga ctgctctttt gggacggctg gctgagctcc gccaacggct acaggctgaa
     3961 cctagacctg aggagcctcc taactaccct gcagcccctg cttctgaccc cctcagagag
     4021 ggcagtggca aggatatgcc taaggtccag ggcttactgg agaatggaga cagtgtgacc
     4081 agtcctgaga aggtagcccc ggaggagggc tcaggtaaga gagatctgga gctgctgtcc
     4141 tcgctgttgc cacagttgac tggccctgtg ttggaactgc ctgaggcaac ccgggccccc
     4201 ttggaggagc tcatgatgga gggggacctg ctcgaggtga ccctggatga gaaccacagc
     4261 atatggcagc tgctgcaggc tggacagccc ccagacctgg agaggatccg cacacttctg
     4321 gagctggaga aggcagagcg tcacgggagt cgggctcggg gccgggccct ggagaggcgg
     4381 cggcggcgga aggtggatcg gggtggggag ggcgatgacc cagcccgaga ggagctagag
     4441 ccaaagaggg tacggagctc agggccagag gctgaggagg tccaggagga ggaagagctg
     4501 gaggaggaga ctgggggtga gggcccccct gcacccatcc ccaccactgg cagccccagc
     4561 acccaggaga accagaatgg cttggaaccg gcggaaggga ccacttcagg cccctcggcc
     4621 cctttctcca ctctgactcc ccggctgcat ctgccctgcc cacagcagcc gcctcagcaa
     4681 cagttgtagt aatgaactag tccgtaactt gaaagtattt cgatttcttg gctttatata
     4741 tcttgtggaa aggacgataa ggtgtagtct taacgtaacg g
//
