LOCUS       sequence-28483-e         872 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 872)
  AUTHORS   Berndt JA, Kim JG, Hudson LD
  TITLE     Identification of cis-regulatory elements in the myelin proteolipid 
            protein (PLP) gene.
  JOURNAL   J Biol Chem. 1992 Jul 25. 267(21):14730-7.
  PUBMED    1378839
REFERENCE   2  (bases 1 to 872)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 1992 Jul 25. 267(21):14730-7."
COMMENT     Sequence Label: pJB044
FEATURES             Location/Qualifiers
     source          1..872
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    673..729
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(738..754)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(738..754)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(751..773)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    762..778
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(786..792,793..810,811..816))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    831..852
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 tggtatatcc agtgattttt ttctccattt tagcttcctt agctcctgaa aatctcgcca
       61 agcttctggt ctctccagcc tccttcttcg gtcttttact ttttctctct gagtatcttt
      121 gtcctcttct cctgcaatga aaagggcttt ctgattgaca gccaaccccc ttaaagggac
      181 aggcctgctt tcctcctcct ctcctcctcc ccttctcctc cccttttccc cacctactca
      241 gggatactcc cttctctaag ggctccatct tctctttctg cctttcactg ccaattgttt
      301 cattttcttt gtttttcctt ttttccctaa ggcttgcctg gaatttcgct ttgctgcaga
      361 gacatctgga aatgtggatt gagggtggaa ataaaatgaa gatgtcctgg aaacttccaa
      421 ctgatccttg ggtctaattg gtgtgggata tgcagcacaa gcgtgagaag ccatgaggtg
      481 agagccccaa caggggctca gagtcaaaaa cctagatatt agctcccaaa gtccttccga
      541 atctgctcca gctccccaca tcactcctag ctgcccttgg ccctctccac ctttcccctc
      601 ccgattcctt ggaaaataag cgttcctccc ccacccccac ccccagctct acgaacacaa
      661 tagagaagaa aaaagcttgc atgcctgcag gtcgactcta gaggatcccc gggtaccgag
      721 ctcgaattcg taatcatgtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
      781 ccncacaaca tacgagccgg aagcataaag tgtaaannct ggggtgccta atgagtgagc
      841 taactcacat taattgcgtt gcgctcactg cc
//
