LOCUS       sequence-284838-        3701 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3701)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 3701)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2805
FEATURES             Location/Qualifiers
     source          1..3701
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             3445..3465
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
ORIGIN
        1 accgccacca tgccgctgac tggtgtagag cccgccagaa tgaacaggaa gaaaggagac
       61 aagggctttg aaagcccaag gccatataaa ttaacccatc aggtcgtctg catcaacaac
      121 ataaatttcc agagaaaatc tgttgtggga tttgtggaac tgactatatt tcccacagtt
      181 gcaaacttga atagaatcaa gttgaacagc aaacagtgta gaatataccg agtaaggatc
      241 aatgatttag aggctgcttt tatttataat gacccaacct tggaagtttg tcacagtgaa
      301 tcaaaacaga gaaacctcaa ttatttttcc aatgcttatg cagctgcagt tagtgctgtg
      361 gaccctgatg caggaaatgg agaactttgc attaaggttc catcagagct atggaaacac
      421 gttgatgagt taaaggtcct gaagatacac atcaattttt ctttggatca gcccaaagga
      481 ggtcttcatt ttgtggtacc cagtgtagag ggaagtatgg cagagagagg tgctcatgtt
      541 ttctcttgtg ggtatcaaaa ttctacaaga ttttggttcc cttgtgttga ttcatactct
      601 gaattgtgta catggaaatt agaatttaca gtagatgctg caatggttgc tgtttctaat
      661 ggcgatttgg tggagacagt gtatactcat gatatgagga agaaaacttt ccattatatg
      721 cttaccattc ctacagcagc gtcaaatatc tccttggcca ttggaccatt tgaaatactg
      781 gtagatccat acatgcatga ggttactcat ttttgtttgc cccaacttct tccattgctg
      841 aaacatacca catcatacct tcatgaagtc tttgaatttt atgaagaaat tcttacatgt
      901 cgttacccat actcctgttt taagactgtc ttcattgatg aggcttatgt tgaagtggct
      961 gcttatgctt ccatgagcat ttttagcaca aatcttttac acagtgccat gattatagat
     1021 gagacacctt tgactagaag gtgtttagcc caatccttgg cccagcagtt ttttggttgt
     1081 ttcatatcta gaatgtcttg gtctgatgaa tgggtgctga agggaatttc aggctatatc
     1141 tatggacttt ggatgaaaaa aacttttggt gttaatgagt accgccattg gattaaagag
     1201 gagctagaca aaatagtggc atatgaacta aaaactggtg gggttttact acatcccata
     1261 tttggtggag gaaaagagaa ggataatccg gcttcccatc tacacttttc aataaagcat
     1321 ccacatacac tgtcctggga atactacagt atgtttcagt gtaaagccca ccttgtgatg
     1381 agattgattg aaaataggat cagtatggaa tttatgctac aagttttcaa taaactgcta
     1441 agtctggcta gtactgcttc atctcagaag ttccagtcac atatgtggag tcagatgttg
     1501 gtttccacat ctgggttttt gaaatccatt tcaaatgtct ctggcaaaga tattcagccg
     1561 ttaataaagc agtgggtaga tcagagtgga gtggtaaaat tttatggaag ttttgcattt
     1621 aatagaaaac gaaatgtctt ggaactggaa ataaaacagg actatacatc tcctggaact
     1681 cagaaatacg tgggaccact taaagtgaca gtgcaggagt tagatggatc cttcaatcat
     1741 acactgcaaa ttgaagaaaa cagccttaaa catgatatac cctgccattc caaaagtaga
     1801 aggaataaaa agaaaaaaat cccactgatg aatggagaag aagttgatat ggatctttct
     1861 gcaatggatg ctgattcccc tttgctgtgg ataaggatag acccagatat gtcagtattg
     1921 aggaaggtag aatttgagca agctgatttt atgtggcagt atcagctccg ctatgagaga
     1981 gatgttgttg cacagcagga atccattttg gctttggaaa aattccctac tccagcatct
     2041 cggcttgcac tcactgatat attagaacaa gagcagtgtt tctacagagt aagaatgtca
     2101 gcttgcttct gtcttgcaaa gattgcaaat tcaatggtga gcacatggac aggaccacca
     2161 gccatgaagt cactcttcac taggatgttt tgttgtaaaa gttgtccaaa cattgtgaaa
     2221 acaaacaact ttatgagctt tcaaagctat tttctacaga agactatgcc agttgcaatg
     2281 gctttattaa gagatgttca taatctttgt cctaaagaag tcttaacatt tattttagac
     2341 ttaatcaagt acaatgacaa caggaaaaat aagttttcag ataactatta tcgtgcagaa
     2401 atgattgatg ccctggccaa ctctgttaca cctgcagtca gtgtgaataa tgaagttaga
     2461 actttggata acttaaatcc tgatgtgcga ctcattcttg aagaaatcac cagatttttg
     2521 aatatggaaa aacttcttcc gagttacagg cataccatca ctgtcagttg tttgagagcc
     2581 atacgggtac ttcagaagaa cggacatgtg ccaagtgatc cagctctttt taaatcttat
     2641 gctgaatatg gccactttgt ggacattagg atagcagctt tggaagcagt tgttgattat
     2701 actaaagtgg acagaagtta tgaagaactg caatggctac ttaatatgat tcagaatgac
     2761 cctgtaccct atgtaaggca taagattctc aacatgttga ctaagaaccc accatttact
     2821 aagaacatgg agtctccctt atgcaatgaa gccctggtag atcaactttg gaaacttatg
     2881 aattctggta cttcacatga ctggaggtta cggtgtggtg ctgtggactt gtacttcaca
     2941 ctttttggcc tcagtagacc ttcctgttta cccttgccag agcttgggtt ggttcttaat
     3001 ctaaaggaga aaaaagctgt cttgaatcct accataattc cagagtcagt agcaggcaac
     3061 caagaagctg caaataatcc aagcagtcac ccacagctag ttggatttca gaaccctttt
     3121 tccagttctc aagatgagga ggagattgat atggatactg ttcatgatag ccaggccttc
     3181 atttcccatc atttaaacat gcttgaaagg ccgtcaactc cagggctctc gaaatatcgg
     3241 ccagctagct cccgatctgc tttaataccc cagcactcag caggctgtga cagcacaccc
     3301 accacaaaac cccagtggag tttggaactt gcacggaagg gaacaggtaa agaacaagca
     3361 cctttggaga tgagtatgca tccagcggca agcgctccac tctcagtctt tactaaggaa
     3421 tctacagcct ccaaacacag tgaccaccat caccaccatc accatgagca caagaaaaag
     3481 aagaagaagc ataaacataa gcacaaacac aagcataagc atgacagtaa agaaaaggac
     3541 aaggagcctt tcactttctc cagccctgcc agtggcaggt ctattcgttc tccttccctt
     3601 tcagactagt aatgaactag tccgtaactt gaaagtattt cgatttcttg gctttatata
     3661 tcttgtggaa aggacgatat tggagtcagt gagccattca c
//
