LOCUS       sequence-28484-e         878 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 878)
  AUTHORS   Berndt JA, Kim JG, Hudson LD
  TITLE     Identification of cis-regulatory elements in the myelin proteolipid 
            protein (PLP) gene.
  JOURNAL   J Biol Chem. 1992 Jul 25. 267(21):14730-7.
  PUBMED    1378839
REFERENCE   2  (bases 1 to 878)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 1992 Jul 25. 267(21):14730-7."
COMMENT     Sequence Label: pJB042
FEATURES             Location/Qualifiers
     source          1..878
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    466..522
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(531..547)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(531..547)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(544..566)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    555..571
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(579..585,586..603,604..609))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    624..645
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     714..733
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(814..831)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tcaacggtgg tntatccngt gatttttttc tccattttag cttccttagc tcctgaaaat
       61 ctcgccaagc ttctggtctc tccagcctcc ttcttcggtc ttttactttt tctctctgag
      121 tatctttgtc ctcttctcct gcaatgaaaa gggctttctg attgacagcc aaccccctta
      181 aagggacagg cctgctttcc tcctcctctc ctcctcccct tctcctcccc ttttccccac
      241 ctactcaggg atactccctt ctctaagggc tccatcttct ctttctgcct ttcactgcca
      301 attgtttcat tttctttgtt tttccttttt tccctaaggc ttgcctggaa tttcgctttg
      361 ctgcagagac atctggaaat gtggattgag ggtggaaata aaatgaagat gtcctggaaa
      421 cttccaactg atccttgggt ctaattggtg tgggatatgc agcacaagct tgcatgcctg
      481 caggtcgact ctagaggatc cccgggtacc gagctcgaat tcgtaatcat gtcatagctg
      541 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
      601 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
      661 ctgcccgctt tccagtcggg aaacctgtcg tgccagatac tggcttaact atgcggcatc
      721 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag
      781 gagaaaatac cgcatcangc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
      841 cgttcggctg cggcgagcgg tatcagctca ctcaaagg
//
