LOCUS       sequence-28485-e         876 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Berndt JA, Kim JG, Hudson LD
  TITLE     Identification of cis-regulatory elements in the myelin proteolipid 
            protein (PLP) gene.
  JOURNAL   J Biol Chem. 1992 Jul 25. 267(21):14730-7.
  PUBMED    1378839
REFERENCE   2  (bases 1 to 876)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 1992 Jul 25. 267(21):14730-7."
COMMENT     Sequence Label: pJB050
FEATURES             Location/Qualifiers
     source          1..876
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    427..483
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(492..508)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(492..508)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(505..527)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    516..532
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(540..546,547..564,565..570))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    585..606
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     675..694
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(775..792)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cggtggtata tccngtgatt tttttctccn ttttagcttc cttagctcct gaaaatctcg
       61 ccaagcttct ggtctctcca gcctccttct tcggtctttt actttttctc tctgagtatc
      121 tttgtcctct tctcctgcaa tgaaaagggc tttctgattg acagccaacc cccttaaagg
      181 gacaggcctg ctttcctcct cctctcctcc tccccttctc ctcccctttt ccccacctac
      241 tcagggatac tcccttctct aagggctcca tcttctcttt ctgcctttca ctgccaattg
      301 tttcattttc tttgtttttc cttttttccc taaggcttgc ctggaatttc gctttgctgc
      361 agagacatct ggaaatgtgg attgagggtg gaaataaaat gaagatgtcc tggaaacttc
      421 caactgaagc ttgcatgcct gcaggtcgac tctagaggat ccccgggtac cgagctcgaa
      481 ttcgtaatca tgtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
      541 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
      601 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagata
      661 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga
      721 aataccgcac agatgcgtaa ggagaaaata ccgcatcang cgctcttccg cttcctcgct
      781 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
      841 ggtaatacgg ttatccacag aatcnnggna taacgc
//
