LOCUS       sequence-284852-       15088 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15088)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 15088)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2819
FEATURES             Location/Qualifiers
     source          1..15088
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    10945..11533
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10998..11018)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(11416..11427)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             11605..11838
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    11916..12037
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11953..11972)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12007..12026
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      12077..12212
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(12232..12251)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(12233..12251)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(12261..12278)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(12261..12277)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12270..12292)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      12419..12874
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(12506..12525)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     12716..12737
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        12900..13004
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(13005..13073,13074..13865)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(13223..13242)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      14036..14624
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     14525..14544
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     14778..14795
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    14912..14933
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(14948..14953,14954..14971,14972..14978)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    14986..15002
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     14991..15013
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     15010..15026
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     15010..15026
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     15045..15065
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        15047..15065
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatgttg ccctgtaaga agagaagaac
     4141 tacagtgaca gagtccctac agcataaagg caatcaagag gaaaacaacg tagacctaga
     4201 atcagccgtt aaaccagaat ctgaccaggt taaggacttg agttcggtgt cactatcctg
     4261 ggatccaagt catggcagag tagctggctt cgaagtacag tctttgcagg atgcaggaaa
     4321 tcagcttggt atggaggata catctctgag ctctggaatg ctcacccaga acacaaatgt
     4381 accaattcta gaaggtgttg atgtggccat ctctcaggga atcaccctac cttccttgga
     4441 gtcttttcac ccccttaata tacacattgg taaaggaaaa ctccacgcta ctggctcaaa
     4501 gagagggaaa aaaatgacac tcaggcctgg gccagttacc caagaagaca gatgtgatca
     4561 tcttacccta aaggagcctt tttcaggaga gcctagtgaa gaagtcaagg aagaaggagg
     4621 gaaacctcaa atgaattctg aaggggagat accttccctg ccatcaggca gccaatctgc
     4681 aaaaccagta agccagccca ggaaatcaac ccagccagat gtttgtgcct ctcctcaaga
     4741 aaagccactc aggactctgt ttcaccaacc tgaggaagag atagaagatg gtggactctt
     4801 cattccaatg gaagaacaag acaatgaaga aagtgagaaa aggagaaaaa agaaaaaggg
     4861 taccaagagg aaacgagatg gaaggggtca agaagggacc ttggcatatg acctgaaact
     4921 ggatgacatg cttgaccgta ccttggagga tggtgccaag cagcacaatc taacagcagt
     4981 caatgtccga aacatccttc atgaagtaat cacaaatgaa cacgtggtag ctatgatgaa
     5041 agcagccatc agtgagacgg aagatatgcc aatgtttgag cctaaaatga cacgctctaa
     5101 actgaaggaa gtagtggaaa aaggagtggt aattccaaca tggaatattt caccaattaa
     5161 gaaggccaat gaaattaagc ctcctcagtt tgtggatatc caccttgaag aagatgattc
     5221 ctcagatgaa gaataccagc cggatgatga agaagaagat gaaactgctg aagagagctt
     5281 attggaaagt gatgttgaaa gcactgcttc atctccacgt ggggcaaaga aatccagatt
     5341 gaggcagtct tctgagatga ctgaaacaga tgaggagagt ggcatattat cagaggctga
     5401 gaaagtcacc acaccagcca tcaggcacat cagtgctgag gtagtgccca tggggccccc
     5461 gccccctcca aagccgaaac agaccagaga tagtactttc atggagaagt tacatgcggt
     5521 agatgaggag ctggcttcca gtccagtctg catggattct ttccagccca tggatgacag
     5581 tctcattgca tttcgaacgc gttctaagat gcccctgaaa gatgttcccc tgggccaatt
     5641 agaggcagag ctccaagctc cagacatcac tccagatatg tatgacccca atacggcaga
     5701 tgatgaggac tggaagatgt ggctgggggg acttatgaat gatgatgtgg ggaatgaaga
     5761 tgaagcagat gatgatgatg atccagaata taatttcctg gaagacctcg atgaaccaga
     5821 cacagaggat ttccggactg accgggcagt gagaatcacc aaaaaggaag taaatgagct
     5881 gatggaagag ctgtttgaaa ctttccaaga tgagatggga ttctccaaca tggaagatga
     5941 tggcccagaa gaggaggagt gtgtagctga gcctcgtcct aactttaaca cccctcaagc
     6001 tctacggttt gaggaaccac tggccaacct gttaaatgaa caacatcgga cagtgaagga
     6061 gctatttgaa cagctgaaga tgaagaaatc ttcagccaaa cagctgcagg aagtagagaa
     6121 ggttaaaccc cagagtgaga aagttcatca gactctgatt ctggacccag cacagaggaa
     6181 gagactccag cagcagatgc agcagcacgt tcagctcttg acccaaatcc accttcttgc
     6241 cacctgcaac cccaacctca atccggaggc cactaccacc aggatatttc ttaaagagct
     6301 gggaaccttt gctcaaagct ccatcgccct tcaccatcag tacaacccca agtttcagac
     6361 cctgttccaa ccctgtaact tgatgggagc tatgcagctg attgaagact tcagcacaca
     6421 tgtcagcatt gactgcagcc ctcataaaac tgtcaagaag actgcgaatg aatttccctg
     6481 tttgccaaag caagtggctt ggattctggc cacaagcaag gttttcatgt atccagagtt
     6541 acttccagtg tgttccctga aggcaaagaa tccccaggat aagatcgtct tcaccaaggc
     6601 tgaggacaat ttgttagctt taggactgaa gcattttgaa ggaactgagt ttcctaatcc
     6661 tctaatcagc aagtaccttc taacctgcaa aactgcccac caactgacag tgagaatcaa
     6721 gaacctcaac atgaacagag ctcctgacaa catcattaaa ttttataaga agaccaaaca
     6781 gctgccagtc ctaggaaaat gctgtgaaga gatccagcca catcagtgga agccacctat
     6841 agagagagaa gaacaccggc tcccattctg gttaaaggcc agtctgccat ccatccagga
     6901 agaactgcgg cacatggctg atggtgctag agaggtagga aatatgactg gaaccactga
     6961 gatcaactca gatcgaagcc tagaaaaaga caatttggag ttggggagtg aatctcggta
     7021 cccactgcta ttgcctaagg gtgtagtcct gaaactgaag ccagttgcca cccgtttccc
     7081 caggaaggct tggagacaga agcgttcatc agtcctgaag cccctcctta tccaacccag
     7141 cccctctctc cagcccagct tcaaccctgg gaaaacacca gcccgatcaa ctcattcaga
     7201 agcccctccg agcaaaatgg tgctccggat tcctcaccca atacagccag ccactgtttt
     7261 acagacagtt ccaggtgtcc ctccactggg ggtcagtgga ggtgagagtt ttgagtctcc
     7321 tgcagcactg cctgctgtgc cccctgaggc caggacaagc ttccctctgt ctgagtccca
     7381 gactttgctc tcttctgccc ctgtgcccaa ggtaatgctg ccctcccttg ccccttctaa
     7441 gtttcgaaag ccatatgtga gacggagacc ctcaaagaga agaggagtca aggcctctcc
     7501 ctgtatgaaa cctgcccctg ttatccacca ccctgcatct gttatcttca ctgttcctgc
     7561 taccactgtg aagattgtga gccttggcgg tggctgtaac atgatccagc ctgtcaatgc
     7621 ggctgtggcc cagagtcccc agactattcc catcactacc ctcttggtta accctacttc
     7681 cttcccctgt ccattgaacc agtcccttgt ggcctcctct gtctcaccct taattgtttc
     7741 tggcaattct gtgaatcttc ctataccatc cacccctgaa gataaggccc acgtgaatgt
     7801 ggacattgct tgtgctgtgg ctgatgggga aaatgccttt cagggcctag aacccaaatt
     7861 agagccccag gaactatctc ctctctctgc tactgttttc ccgaaagtgg aacatagccc
     7921 agggcctcca ctagcagatg cagagtgcca agaaggattg tcagagaata gtgcctgtcg
     7981 ctggaccgtt gtgaaaacag aggaggggag gcaagctctg gagccgctcc ctcagggcat
     8041 ccaggagtct ctaaacaacc ctacccctgg ggatttagag gaaattgtca agatggaacc
     8101 tgaagaagct agagaggaaa tcagtggatc ccctgagcgt gatatttgtg atgacatcaa
     8161 agtggaacat gctgtggaat tggacactgg tgccccaagc gaggagttga gcagtgctgg
     8221 agaagtaacg aaacagacag tcttacagaa ggaagaggag aggagtcagc caactaaaac
     8281 cccttcatct tctcaagagc cccctgatga aggaacctca gggacagatg tgaacaaagg
     8341 atcatcaaag aatgctttgt cctcaatgga tcctgaagtg aggcttagta gccccccagg
     8401 gaagccagaa gattcatcca gtgttgatgg tcagtcagtg gggactccag ttgggccaga
     8461 aactggagga gagaagaatg ggccagaaga agaggaagaa gaggactttg atgacctcac
     8521 ccaagatgag gaagatgaaa tgtcatcagc ttctgaggaa tctgtgcttt ctgtcccaga
     8581 actccaggaa acaatggaga aactgacttg gctggcatct gaaaggcgca tgagtcagga
     8641 gggtgagtct gaagaagaga attctcagga ggagaactct gagccagaag aagaggagga
     8701 agaagaagca gaaggaatgg aaagcctgca gaaagaggat gaaatgacgg atgaagcagt
     8761 tggagactct gctgagaagc ctcctacttt tgcttcacct gagactgctc cagaagtgga
     8821 gaccagcaga actccaccag gagagagcat caaagctgct ggaaaaggcc ggaacaatca
     8881 tcgagctcgc aacaagcggg gaagtcgggc tcgggccagc aaggacacct ccaagctgct
     8941 gttgctgtat gatgaggaca ttctcgagcg agatccactc agggagcaga aggacttggc
     9001 ctttgcccaa gcttatctga ccagggtgcg agaagcccta caacatatcc ctggcaagta
     9061 tgaagacttc cttcaagtca tctatgaatt tgagtcaagt acccagagac ggacggctgt
     9121 agatctctac aaaagcctgc aaattctgct ccaagactgg cctcagctgt tgaaagactt
     9181 tgctgctttc ctgttacctg agcaagctct ggcctgtgga ttatttgagg agcagcaggc
     9241 ttttgagaag agccgcaagt tccttcggca gctggagatt tgctttgcag agaacccctc
     9301 acaccaccag aagattatca aggtcctcca aggctgtgca gactgccttc cccaggagat
     9361 caccgagctc aagacacaga tgtggcagct cctcaagggc cacgaccacc tgcaggatga
     9421 gttttctatc ttctttgacc acttgcgccc agcagctagc cggatgggtg actttgaaga
     9481 gatcaattgg actgaggaaa aggagtatga gtttgatggc tttgaagaag tggccctgcc
     9541 tgatgtggaa gaagaggagg agcctcccaa gatacccaca gcctcaaaga acaagaggaa
     9601 aaaagagatc ggggtccaaa atcatgataa ggagactgaa tggccagatg gggccaagga
     9661 ctgtgcctgc tcctgccatg aaggaggtcc agattccaag ctgaagaaga gcaaaaggcg
     9721 gagctgtagc cactgtagca gcaaggtctg tgacagcaaa tcctacaaga gcaaggagcc
     9781 ccatgagttg gtgggcagca gcccccaccg agaggctagt cctatgcctg gtgctaagga
     9841 agctgggcag ggcaaggata tgatggaaga ggaagcccca gaggagcggg agagcactga
     9901 ggccacccag agcaggactg tcaggaccac cagaaaggga gagatgcctg tttcaggatt
     9961 ggcagtgggg agcactttgc catcccctcg agaagtgact gttacagaac ggctcctcct
    10021 ggatggccca ccacctcatt caccagagac tcctcaattt ccccccacaa ctggagctgt
    10081 actgtacact gttaagagaa accaggttgg gcctgaggtt cgctcctgcc ccaaggcatc
    10141 ccccagactt cagaaagaga gggagggcca aaaggcagtg agtgagtcag aggctttgat
    10201 gctggtctgg gatgcatcag aaactgagaa attgcctggt accgtggaac cccctgcttc
    10261 cttcctgagt cctgtttcct caaagaccag agatgcaggg agaagacatg tgtccgggaa
    10321 accagacact caagagagat ggctgccctc aagcagagct cgggtgaaga caagagacag
    10381 gacgtgccct gtccatgaat ctccatcagg aattgacacc tcagagactt ctcccaaagc
    10441 ccctagaggg ggtttggcta aagacagtgg aacacaggcc aagggtccag agggggagca
    10501 gcagccaaag gccgcagaag ctacggtgtg tgccaacaac agcaaggtca gctccactgg
    10561 ggaaaaggtt gtcctgtgga caagggaagc tgaccgtgtg atcctcacca tgtgccagga
    10621 gcaaggggca cagccacaga ccttcaacat catctcccag cagctgggaa ataagacccc
    10681 tgctgaggtt tcccaccgtt ttcgagaact catgcagctc ttccacactg cctgtgaagc
    10741 cagctctgag gatgaggatg atgcaaccag taccagcaat gcagaccagc tgtctgacca
    10801 tggggacctt ctgtctgaag aggagctgga tgaatagtaa tgaactagtc cgtaacttga
    10861 aagtatttcg atttcttggc tttatatatc ttgtggaaag gacgacatcc ttctgaatgt
    10921 cagatttaaa cgcgttaagt cgacaatcaa cctctggatt acaaaatttg tgaaagattg
    10981 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
    11041 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
    11101 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
    11161 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
    11221 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
    11281 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa
    11341 tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
    11401 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
    11461 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
    11521 gccgcctccc cgcgtcgact ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
    11581 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc
    11641 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
    11701 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
    11761 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
    11821 tgtggaaaat ctctagcagt acgtatagta gttcatgtca tcttattatt cagtatttat
    11881 aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg
    11941 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
    12001 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc
    12061 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
    12121 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
    12181 ccagaagtag tgaggaggct tttttggagg cctagggacg tacccaattc gccctatagt
    12241 gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
    12301 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
    12361 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac
    12421 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
    12481 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
    12541 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
    12601 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
    12661 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
    12721 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
    12781 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
    12841 gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc
    12901 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
    12961 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
    13021 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
    13081 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
    13141 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
    13201 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
    13261 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
    13321 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
    13381 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
    13441 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
    13501 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
    13561 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
    13621 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
    13681 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
    13741 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
    13801 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
    13861 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
    13921 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
    13981 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
    14041 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
    14101 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
    14161 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
    14221 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
    14281 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
    14341 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
    14401 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
    14461 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
    14521 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
    14581 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
    14641 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
    14701 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
    14761 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
    14821 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
    14881 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
    14941 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
    15001 aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt aaccctcact
    15061 aaagggaaca aaagctggag ctgcaagc
//
