LOCUS       sequence-284868-        2486 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2486)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2486)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2864
FEATURES             Location/Qualifiers
     source          1..2486
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggaggggtc tctggcaggc agcctggctg caccagatcg tccccaaggc
       61 ccagagagac tgcctggccc ggcgccaagg gagaacatcg agggtggggc cgaagctgct
      121 gagggggaag gtggcatctt ccggtccacc cgttacctgc ctgtcaccaa ggagggcccc
      181 cgagacattc tggatggcag aggtggcatc tctgtggcca actttgaccc aggcaccttc
      241 agcctgatgc gctgtgactt ctgcggggct ggcttcgaca cacgggccgg cctctccagc
      301 cacgcccggg cccacctacg tgacttcggt atcaccaact gggagctcac tgtctcaccc
      361 atcaacatcc tgcaggagct gctggccacc tctgctgctg agcagccccc cagccccctg
      421 ggccgagagc ctgggggtcc gcctggcagc ttcctgacct cccgtcggcc ccgcttacct
      481 ctcacggtgc cctttccacc cacctgggct gaggaccctg ggccagccta tggagatgcc
      541 tcaggcccag agccagcacg agacatccgc tgcgagttct gtggtgagtt cttcgagaac
      601 cgcaagggcc tctcgagcca cgcgcgctcc catctgcggc aaatgggcgt gaccgagtgg
      661 tacgtcaatg gctcgcccat cgacacgctg cgggagatcc tgaagagacg gacccagtct
      721 cggcctggtg gacctcccaa cccaccaggg ccaagcccaa aagccctggc caagatgatg
      781 ggcggcgcag gtcctggcag ctcactggaa gcccgcagcc cctcggacct tcacatctca
      841 cccttggcca agaagttgcc accaccaccg ggcagccccc tgggccactc accaactgcc
      901 tctcctcctc ctacggcccg aaagatgttc ccaggcctgg ctgcaccctc cttgcccaag
      961 aagctgaagc ctgaacaaat acgggtggag atcaagcggg agatgctgcc gggggccctt
     1021 catggggaac tgcacccatc tgagggtccc tggggggcac cacgggaaga catgacaccc
     1081 ctgaacctgt cgtcccgggc agagccggtg cgcgacatcc gctgtgagtt ctgcggcgag
     1141 ttcttcgaga accgcaaggg cctgtcgagt cacgcgcgct cacacctgcg gcagatgggt
     1201 gtgaccgagt ggtccgtcaa tggttcgccc atcgacacac tgcgagagat cctcaagaag
     1261 aagtccaagc cgtgcctcat caagaaggag ccaccggctg gagacctggc ccctgccctg
     1321 gctgaggacg ggcctcccac cgtggcccct gggcccgtgc agtccccact gccgctgtcg
     1381 cccctggctg gccggccagg caaaccaggt gcagggccgg cccaggttcc tcgtgagctc
     1441 agcctgacgc ccatcactgg ggccaagccc tcagccactg gctacctggg ctcagtggca
     1501 gccaagcggc ccctgcagga ggaccgcctc ctcccagcag aggtcaaggc caagacctac
     1561 atccagactg aactgccctt caaggcaaag acccttcatg agaagacctc ccactcctcc
     1621 accgaggcct gctgcgagct gtgtggcctt tactttgaaa accgcaaggc cctggccagc
     1681 cacgcacggg cacacctgcg gcagttcggc gtgaccgagt ggtgcgtcaa tggctcgccc
     1741 atcgagacac tgagcgagtg gatcaaacac cggccccaga aggtgggcgc ctaccgcagc
     1801 tacatccagg gcggccgccc cttcaccaag aagttccgca gtgccggcca tggccgtgac
     1861 agtgacaagc ggccgtccct ggggctggca cccgggggcc tggccgtggt cggccgcagt
     1921 gccggagggg agccagggcc cgaggctggc cgggcagccg acggtggtga gcggcctctg
     1981 gcagccagcc cgccaggcac cgtgaaggct gaggagcacc agcggcagaa catcaacaaa
     2041 tttgaacgcc gacaagcccg ccctccagat gcctccgcag cccggggagg cgaggacacc
     2101 aatgacctac agcagaagct ggaggaggtg cggcaacccc caccccgagt ccggccagtc
     2161 ccctccctgg tgccccggcc cccccagaca tcacttgtca agttcgtggg caacatctac
     2221 accctcaaat gcaggttctg tgaggtggaa ttccagggcc ccctctccat ccaggaagag
     2281 tgggtgcggc acttacagcg gcacatcctg gagatgaact tctccaaagc ggacccccca
     2341 cctgaggagt cccaggcccc gcaggcacag acagcggcgg cagaggctcc ctagtaatga
     2401 actagtccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac
     2461 gattatagct gggagacaaa ccaata
//
