LOCUS       sequence-284883-        4862 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4862)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 4862)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2885
FEATURES             Location/Qualifiers
     source          1..4862
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggagacgtc tgcctcagcc actgcctccg agaagcaaga agccaaaagt
       61 gggatcctgg aggccgctgg cttccccgac ccgggtaaaa aggcctctcc tttggtggtg
      121 gctgcagcgg cagcagcagc ggtagctgcc caaggagtgc cgcagcatct cttgccacca
      181 ttccatgcgc ccctaccgat tgacatgcga caccaggaag gaaggtacca ttacgagcct
      241 cattctgtcc acggtgtgca cgggccccct gccctcagcg gcagccctgt catctctgac
      301 atctccttga tccggctttc cccgcacccg gctggccctg gggagtcccc cttcaacgcc
      361 ccccacccgt acgtgaaccc ccacatggag cactacctcc gttctgtgca cagcagcccc
      421 acgctctcca tgatctctgc agccaggggc ctcagccccg ctgatgtggc ccaggagcac
      481 cttaaggaga ggggactgtt tggccttcct gctccaggca ccaccccctc agactattac
      541 caccagatga ccctcgtggc aggccacccc gcgccctacg gggacctgct gatgcagagc
      601 gggggcgctg ccagcgcacc ccatctccac gactacctca accccgtgga cgtgtcccgt
      661 ttctccagcc cgcgggtgac gccccgcctg agccgcaagc gggcgctgtc catctcccca
      721 ctctcagacg ccagcctgga cctgcagcgg atgatccgca cctcacccaa ctcgctagtg
      781 gcctacatca acaactcccg aagcagctca gcggccagcg gttcctacgg gcatctgtca
      841 gcgggtgccc tcagcccagc cttcaccttc ccccacccca tcaaccccgt ggcctaccag
      901 cagattctga gccagcagag gggtctgggg tcagcctttg gacacacacc acccctgatc
      961 cagccctcac ccaccttcct ggcccagcag cccatggccc tcacctccat caatgccacg
     1021 cccacccagc tcagcagcag cagcaactgt ctgagtgaca ccaaccagaa caagcagagc
     1081 agtgagtcgg ccgtcagcag caccgtcaac cctgtcgcca ttcacaagcg cagcaaggtc
     1141 aagaccgagc ctgagggcct gcggccggcc tcccctctgg cgctgacgca gggccaggtg
     1201 tctggacacg gctcatgtgg gtgtgccctt cccctctccc aggagcagct ggctgacctc
     1261 aaggaagatc tggacaggga tgactgtaag caggaggctg aggtggtcat ctatgagacc
     1321 aactgccact gggaagactg caccaaggag tacgacaccc aggagcagct ggtgcatcac
     1381 atcaacaacg agcacatcca cggggagaag aaggagtttg tgtgccgctg gcaggcctgc
     1441 acgcgggagc agaagccctt caaggcgcag tacatgctgg tggtgcacat gcggcgacac
     1501 acgggcgaga agccccacaa gtgcacgttc gagggctgct cgaaggccta ctcccgcctg
     1561 gagaacctga agacacacct gcggtcccac accggggaga agccatatgt gtgtgagcac
     1621 gagggctgca acaaagcctt ctccaacgcc tcggaccgcg ccaagcacca gaatcgcacc
     1681 cactccaacg agaaacccta catctgcaag atcccaggct gcaccaagag atacacagac
     1741 cccagctctc tccggaagca tgtgaaaacg gtccacggcc cagatgccca cgtcaccaag
     1801 aagcagcgca atgacgtgca cctccgcaca ccgctgctca aagagaatgg ggacagtgag
     1861 gccggcacgg agcctggcgg cccagagagc accgaggcca gcagcaccag ccaggccgtg
     1921 gaggactgcc tgcacgtcag agccatcaag accgagagct ccgggctgtg tcagtccagc
     1981 cccggggccc agtcgtcctg cagcagcgag ccctctcctc tgggcagtgc ccccaacaat
     2041 gacagtggcg tggagatgcc ggggacgggg cccgggagcc tgggagacct gacggcactg
     2101 gatgacacac ccccaggggc cgacacctca gccctggctg ccccctccgc tggtggcctc
     2161 cagctgcgca aacacatgac caccatgcac cggttcgagc agctcaagaa ggagaagctc
     2221 aagtcactca aggattcctg ctcatgggcc gggccgactc cacacacgcg gaacaccaag
     2281 ctgcctcccc tcccgggaag tggctccatc ctggaaaact tcagtggcag tgggggcggc
     2341 gggcccgcgg ggctgctgcc gaacccgcgg ctgtcggagc tgtccgcgag cgaggtgacc
     2401 atgctgagcc agctgcagga gcgccgcgac agctccacca gcacggtcag ctcggcctac
     2461 accgtgagcc gccgctcctc cggcatctcc ccctacttct ccagccgccg ctccagcgag
     2521 gcctcgcccc tgggcgccgg ccgcccgcac aacgcgagct ccgctgactc ctacgacccc
     2581 atctccacgg acgcgtcgcg gcgctcgagc gaggccagcc agtgcagcgg cggctccggg
     2641 ctgctcaacc tcacgccggc gcagcagtac agcctgcggg ccaagtacgc ggcagccact
     2701 ggcggccccc cgcccactcc gctgccgggc ctggagcgca tgagcctgcg gaccaggctg
     2761 gcgctgctgg acgcgcccga gcgcacgctg cccgccggct gcccacgccc actggggccg
     2821 cggcgtggca gcgacgggcc gacctatggc cacggccacg cgggggctgc gcccgccttc
     2881 ccccacgagg ctccaggcgg cggagccagg cgggccagcg accctgtgcg gcggcccgat
     2941 gccctgtccc tgccgcgggt gcagcgcttc cacagcaccc acaacgtgaa ccccggcccg
     3001 ctgccgccct gtgccgacag gcgaggcctc cgcctgcaga gccacccgag caccgacggc
     3061 ggcctggccc gcggcgccta ctcgccccgg ccgcctagca tcagcgagaa cgtggcgatg
     3121 gaggccgtgg cggcaggagt ggacggcgcg gggcccgagg ccgacctggg gctgccggag
     3181 gacgacctgg tgcttccaga cgacgtggtg cagtacatca aggcgcacgc cagtggcgct
     3241 ctggacgagg gcaccgggca ggtgtatccc acggaaagca ctggcttctc tgacaacccc
     3301 agactaccca gcccggggct gcacggccag cgcaggatgg tggctgcgga ctccaacgtg
     3361 ggcccctccg cccctatgct gggaggatgc cagttaggct ttggggcgcc ctccagcctg
     3421 aacaaaaata acatgcctgt gcagtggaat gaggtgagct ccggcaccgt agacgccctg
     3481 gccagccagg tgaagcctcc accctttcct cagggcaacc tggcggtggt gcagcagaag
     3541 cctgcctttg gccagtaccc gggctacagt ccgcaaggcc tacaggctag ccctgggggc
     3601 ctggacagca cgcagccaca cctgcagccc cgcagcggag ccccctccca gggcatcccc
     3661 agggtaaact acatgcagca gctgcgacag ccagtggcag gcagccagtg tcctggcatg
     3721 actaccacta tgagccccca tgcctgctat ggccaagtcc acccccagct gagccccagc
     3781 accatcagtg gggccctcaa ccagttcccc caatcctgca gcaacatgcc agccaagcca
     3841 gggcatctgg ggcaccctca gcagacagaa gtggcacctg accccaccac gatgggcaat
     3901 cgccacaggg aacttggggt ccccgattca gccctggctg gagtgccacc acctcaccca
     3961 gtccagagct acccacagca gagccatcac ctggcagcct ccatgagcca ggagggctac
     4021 caccaggtcc ccagccttct gcctgcccgc cagcctggct tcatggagcc ccaaacaggc
     4081 ccgatggggg tggctacagc aggctttggc ctagtgcagc cccggcctcc cctcgagccc
     4141 agccccactg gccgccaccg tggggtacgt gctgtgcagc agcagctggc ctacgccagg
     4201 gccacaggcc atgccatggc tgccatgccg tccagtcagg aaacagcaga ggctgtgccc
     4261 aagggagcga tgggcaacat ggggtcggtg cctccccagc cgcctccgca ggacgcaggt
     4321 ggggccccgg accacagcat gctctactac tacggccaga tccacatgta cgaacaggat
     4381 ggaggcctgg agaacctcgg gagctgccag gtcatgcggt cccagccacc acagccacag
     4441 gcctgtcagg acagcatcca gccccagccc ttgccctcac caggggtcaa ccaggtgtcc
     4501 agcactgtgg actcccagct cctggaggcc ccccagattg acttcgatgc catcatggat
     4561 gatggcgatc actcgagttt gttctcgggt gctctgagcc ccagcctcct ccacagcctc
     4621 tcccagaact cctcccgcct caccaccccc cgaaactcct tgaccctgcc ctccatcccc
     4681 gcaggcatca gcaacatggc tgtcggggac atgagctcca tgctcaccag cctcgccgag
     4741 gagagcaagt tcctgaacat gatgacctag taatgaacta gtccgtaact tgaaagtatt
     4801 tcgatttctt ggctttatat atcttgtgga aaggacgatt agtctttgta ctcaccttgg
     4861 cg
//
