LOCUS       sequence-284889-        3746 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3746)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 3746)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2889
FEATURES             Location/Qualifiers
     source          1..3746
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tgggggtgga ctttgatgtg aagactttct gccacaactt gcgggcgact
       61 aagccaccat acgagtgccc ggtggagacc tgccgaaagg tctacaagag ttacagtggt
      121 attgagtacc acctgtacca ctatgaccac gacaacccac cacccccaca acaaactcca
      181 ctccgcaagc acaagaaaaa ggggcgccag tcacgcccag ccaacaagca gtcacccagc
      241 ccctcagagg tctcacagtc accaggccgt gaggtgatga gctatgcaca ggcccagcgc
      301 atggtggagg tggacttgca tggccgcgtc caccgcatca gcatctttga caacctggat
      361 gtggtgtcag aggatgagga agcccccgag gaggcccctg agaatggcag caacaaggag
      421 aacactgaga caccagctgc tactcccaag tcaggcaaac ataagaacaa ggagaagcgc
      481 aaggactcca accatcacca ccaccacaat gtttctgcga gcaccactcc caagctgcca
      541 gaggtggtct atcgggagct ggaacaggac acccctgatg ccccaccccg gccaacttcc
      601 tattaccggt acatcgagaa gtctgcagag gagctggacg aggaagtaga gtatgacatg
      661 gacgaggagg actacatctg gctggatatc atgaatgagc gtcggaagac agagggtgta
      721 agtcccatcc cgcaggagat ctttgagtac ctaatggacc gactggagaa ggagtcgtac
      781 tttgagagtc ataataaagg cgaccctaat gcgctagtgg acgaggatgc tgtttgctgt
      841 atctgcaatg atggtgagtg ccagaacagc aatgtcatcc tcttctgtga catgtgcaac
      901 ctggccgtgc accaggagtg ctacggtgtc ccctatatcc ctgagggcca gtggctgtgc
      961 cgccgttgcc tgcagtcacc ctctcgtgct gtggattgtg ccctgtgccc caacaagggc
     1021 ggtgccttca agcagacaga tgacgggcgc tgggcccatg tggtgtgtgc cttgtggatc
     1081 cctgaggtct gcttcgccaa cacggtcttc ctagagccta ttgacagcat tgagcacatc
     1141 ccaccagctc gctggaagct cacctgctac atttgcaaac aacggggctc aggggcctgc
     1201 atccagtgcc acaaggccaa ctgttacaca gctttccatg tgacatgcgc ccagcaggct
     1261 ggcctttaca tgaagatgga gcctgtgcgg gagacaggcg ccaacggcac ctctttcagt
     1321 gtccgcaaga cagcctactg cgacatccac acgcctccag gttcagcacg ccgactgcct
     1381 gccctgtccc acagcgaggg tgaggaggat gaagatgagg aggaggatga gggtaagggc
     1441 tggagctcag agaaagtcaa gaaggccaag gccaagtccc ggatcaaaat gaagaaggca
     1501 cggaagatcc tggcagagaa gcgggcagca gcacctgtgg tgtcagtgcc ctgcatccca
     1561 ccacacaggc ttagtaaaat caccaaccgc ctgaccatcc aaaggaagag ccagttcatg
     1621 cagaggctgc acagctactg gacactgaag cggcagtcac ggaatggggt cccattgcta
     1681 cgtcgcctgc agacacacct gcaatctcag aggaactgtg accaagttgg gagagattct
     1741 gaggataaga actgggccct taaagaacag ctcaagtcct ggcagcggct ccggcatgac
     1801 ttggagcgag ctcggctgct cgtggaattg atccgcaagc gggaaaaact caaaagggag
     1861 acgatcaagg ttcagcagat tgccatggag atgcagctga ctcctttcct catcctcctt
     1921 cgcaaaacct tggagcagct ccaagagaag gacacaggca acatcttcag cgagccggtc
     1981 cctctgtctg aggtacctga ctacctagac cacatcaaaa agcccatgga ctttttcacc
     2041 atgaagcaga acttggaggc ttaccgctac ctgaattttg atgattttga ggaggacttc
     2101 aacctcatcg tcagcaactg cctcaagtat aacgccaagg acaccatctt ctaccgggca
     2161 gcagtgcggc ttcgtgagca gggtggtgct gtgctccgcc aggcccggcg ccaggcagaa
     2221 aaaatgggca ttgactttga gacgggcatg catatccccc acagcctggc tggagatgag
     2281 gccacacacc acactgaaga tgcagccgag gaagagcggc tggtcttgct ggagaaccag
     2341 aagcacctgc cagtggaaga acagctaaag ctgcttctgg agcggctgga cgaagtgaat
     2401 gccagcaagc agagtgtggg ccgctcacgg cgtgcaaaga tgatcaagaa agagatgacg
     2461 gcactgcggc ggaagcttgc ccatcagcga gagacgggac gtgatggccc tgagcggcat
     2521 ggcccctcga gccggggtag tctgacaccc cacccggcag cctgtgacaa ggatgggcag
     2581 acagatagtg cggcagagga gagcagcagc caggagacaa gcaaaggcct gggtcccaac
     2641 atgtcctcaa cccccgcaca tgaggtgggc aggagaacct cagttctgtt ctccaaaaag
     2701 aacccgaaga cagctggacc gcccaagagg ccgggccggc cccccaaaaa ccgggagagc
     2761 cagatgaccc ccagccacgg aggcagtcct gtggggcccc cccagctccc catcatgagt
     2821 tccctgcgtc agcgcaagcg gggtaggagc ccccggccca gttcgagctc agacagcgac
     2881 agtgataagt ccacagaaga ccccccaatg gacttaccag ccaatggctt cagcggtgga
     2941 aaccaaccag tgaagaagag tttcttggta taccgtaatg actgcagcct tccccggagc
     3001 agctcagact ctgagtccag cagcagtagc agtagcagcg ctgcttcaga ccggaccagc
     3061 acaacgccct caaaacaagg ccggggcaaa ccctccttct ctcggggcac tttcccagag
     3121 gacagcagtg aggatacctc aggcactgag aatgaggcct actccgtggg cactggccgc
     3181 ggcgtgggcc acagcatggt aaggaagagt ctgggccggg gagctggctg gctgtcagag
     3241 gatgaggact ccccgctgga tgctctggac ctcgtgtggg ccaaatgccg aggctatcca
     3301 tcatacccag ctctgatcat tgatccaaag atgccccgag aaggtatgtt ccaccatggg
     3361 gttcccatcc ctgtgccccc actggaggtg ctgaaacttg gggagcagat gacccaggaa
     3421 gcccgagagc atctctacct cgtcctcttc tttgacaaca aacgaacctg gcagtggctg
     3481 cccaggacca agctggttcc tctgggtgtg aaccaggacc tagacaagga gaagatgctg
     3541 gagggccgca agtccaacat ccgcaagtca gtacagatcg cctaccacag ggctctgcag
     3601 caccgcagca aggtgcaagg cgagcagagc agtgagacca gcgatagtga ttagtaatga
     3661 actagtccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac
     3721 gacttaaggg tgcggctgta ttccca
//
