LOCUS       sequence-28491-e         948 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 948)
  AUTHORS   Berndt JA, Kim JG, Hudson LD
  TITLE     Identification of cis-regulatory elements in the myelin proteolipid 
            protein (PLP) gene.
  JOURNAL   J Biol Chem. 1992 Jul 25. 267(21):14730-7.
  PUBMED    1378839
REFERENCE   2  (bases 1 to 948)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 1992 Jul 25. 267(21):14730-7."
COMMENT     Sequence Label: pJB038
FEATURES             Location/Qualifiers
     source          1..948
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(159..175)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(159..175)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(172..194)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    183..199
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(207..213,214..231,232..237))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    252..273
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     342..361
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(442..459)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(693..712)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ggtggtatat ccngtgattt ttttctccnt tttagcttcc ttagctcctg aaaatctcgc
       61 caagcgaaaa gggctttctg attgacagcc aacccgcttg catgcctgca ggtcgactct
      121 agaggatccc cgggtaccga gctcgaattc gtaatcatgt catagctgtt tcctgtgtga
      181 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
      241 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
      301 cagtcgggaa acctgtcgtg ccagatactg gcttaactat gcggcatcag agcagattgt
      361 actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg
      421 catcaggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
      481 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
      541 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
      601 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
      661 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
      721 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
      781 cccttcggga agcgtggcgc tttctcatag ctcacgctgt angtatctca gttcggtgta
      841 ngtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg ancgctgcgc
      901 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgactt
//
