LOCUS       sequence-284919-        1364 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1364)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1024
FEATURES             Location/Qualifiers
     source          1..1364
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggccctcta ctgcggcgac aacttcggag tgtattcgca gcccggcctg
       61 cccccgcccg ccgccaccgc cgccgccccg ggcgcccccc cggcagccag ggcgccttac
      121 gggctggccg actacgccgc gccgccggcc gccgccgcca acccctacct gtggctcaac
      181 gggcccggcg tgggaggccc gccctccgcc gccgccgcag ccgccgccgc gtacctgggc
      241 gctccgccgc cgccgccgcc ccccggggcc gcggccgggc cctttctgca gccaccgcct
      301 gccgccggca ccttcggctg ctctcagcgg cccttcgcgc agcccgcgcc cgccgcgccc
      361 gcctcgcccg ccgcgcccgc ggggcccggg gagctgggct ggctgtccat ggccagccgc
      421 gaggacctga tgaagatggt gcggccgccc tactcgtatt cggcgctcat cgccatggcc
      481 attcagagcg cgcccgagcg caaactcact ctcagccaca tctaccagtt cgtcgccgat
      541 agcttcccct tctaccagcg cagcaaggcc ggctggcaga actccatccg ccacaacctg
      601 tcgctcaacg actgcttcaa gaaggtgccc cgcgacgagg acgacccagg aaagggtaat
      661 tattggactc ttgatccgaa ctgcgagaaa atgtttgaca atgggaactt ccgtcggaag
      721 cgaaagcgcc gctctgaggc cagcaatggc tccacagtgg ctgctgggac atcaaagtcc
      781 gaagaagggc tctcctcagg attggggtct ggagtgggtg ggaagccaga agaagagagc
      841 ccctccactc tgctgaggcc ttcccactcc ccagagcctc cggagggcac caagagtact
      901 gcctcatctc ctggaggacc catgctcacc tccacccctt gtctcaacac tttcttcagc
      961 agcctcagct ccttgagtgt cagcagcagt gtgagtaccc agcgggctct ccctggcagc
     1021 cgccacctag ggatccaggg ggcccagctg ccctccagcg gcgtgttctc cccgacctcc
     1081 atctcagagg cctcagcaga caccttgcaa ctgagcaata gcaccagcaa tagcaccggc
     1141 cagagatctt cctattacag ccctttccct gccagcacca gcggggggca aagcagcccc
     1201 ttcagcagcc ctttccacaa ctttagcatg gtgaacagcc tcatctaccc ccgagagggc
     1261 tccgaggtgt agtaatgaac tagtccgtaa cttgaaagta tttcgatttc ttggctttat
     1321 atatcttgtg gaaaggacga tgttcaagga gcgcccacag gaaa
//
