LOCUS       sequence-284928-        2027 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2027)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2027)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1049
FEATURES             Location/Qualifiers
     source          1..2027
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     RBS             731..739
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 accgccacca tggccacagg gctcctgaga gccaaaaaag aggcgttcgt ggcattcagg
       61 gatgtggctg tgtacttcac ccaggaggag tggaggttgt tgagccctgc tcagaggacc
      121 ctgcacaggg aggtgatgct ggagacttat aaccatctgg tctcactgga aattccatct
      181 tctaaaccaa aactcattgc tcagctggag cgaggggaag cgccctggag agaggagaga
      241 aaatgtccac tggacctctg tccagcagaa tcgaagccag aaattcaact tagtccctcc
      301 tgccctctga ttttctccag tcagcaagct ctcagccaac atgtgtggct gagtcatctc
      361 tctcagctgt tttcaagttt atgggcagga aatcctctcc acctgggaaa acactatcca
      421 gaagatcaga aacaacagca ggatccattc tgctttagtg gcaaagcaga atggattcaa
      481 gagggagaag actccagact cctgtttggg agagtaagca aaaatggcac ttcaaaggca
      541 ctttccagcc cacctgaaga acaacagcca gcacagtcca aggaagacaa cacagtggtg
      601 gatatagggt ccagccctga acggagggca gatctagagg aaacagacaa agtattgcat
      661 ggtttagaag tctcaggatt tggagaaatc aaatatgaag agtttgggcc aggctttatc
      721 aaggagtcaa acctccttag cctccagaag acacaaactg gggagacacc ttacatgtac
      781 actgagtggg gagacagctt tggcagtatg tcagtcctca tcaaaaaccc aaggacacac
      841 tctgggggaa agccttatgt gtgcagggaa tgtgggcgag gctttacgtg gaagtcaaac
      901 ctgatcacac atcagaggac acactcaggg gagaaacctt atgtgtgcaa ggattgtgga
      961 cgaggcttta cttggaagtc gaacctcttt acacatcagc ggacacactc agggctcaag
     1021 ccttatgtgt gcaaggaatg tgggcagagc tttagcctga agtcaaacct cattacccac
     1081 cagagggcgc acactgggga gaagccttat gtttgcaggg aatgtgggcg tggctttcgc
     1141 cagcattcac acctggtcag acacaagagg acacattcag gagagaagcc ttacatttgc
     1201 agggagtgtg agcaaggctt tagccagaag tcacacctca tcagacactt aaggacacac
     1261 acaggagaga agccttatgt atgcacagaa tgtgggcgtc actttagctg gaaatcaaac
     1321 ctcaaaacac accagaggac acactcaggg gttaaacctt atgtctgcct ggagtgcggg
     1381 cagtgcttta gcctgaagtc aaaccttaac aaacaccaga ggtcacacac gggggagaag
     1441 ccatttgtat gtacggagtg tgggcgaggc tttacccgga aatcaaccct gagcacgcac
     1501 cagaggacac actcagggga gaagccattt gtatgtgctg agtgtggacg aggctttaat
     1561 gataagtcca ccctcatttc acaccagagg acacattcag gggaaaagcc ttttatgtgc
     1621 agggagtgtg gcagaaggtt tcggcagaag cctaacctgt ttaggcacaa gagggcacac
     1681 tcaggtgcct ttgtgtgcag ggagtgtggg caaggctttt gtgctaagtt aactctcatt
     1741 aaacaccaga gagcacacgc aggggggaag cctcatgtgt gcagggagtg tgggcaaggc
     1801 tttagccggc agtcacacct cattagacac cagaggacac attcaggaga gaagccttat
     1861 atttgcagaa agtgtggacg gggctttagt cggaagtcca accttatcag acatcagagg
     1921 acacactcag gatagtaatg aactagtccg taacttgaaa gtatttcgat ttcttggctt
     1981 tatatatctt gtggaaagga cgatgtgcga gccggcctct gaggcaa
//
