LOCUS       sequence-284943-        1790 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1790)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1199
FEATURES             Location/Qualifiers
     source          1..1790
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggatccgca gcctcctcca cccgcccagg gcagcccacc tcaccgtggc
       61 cgaggccggg gccgtggccg aggccgtggt cggggccgtg gccgtggcag ggggggcgct
      121 ggagccccta gggcgcccct gccctgcccc acctgcggcc gcctcttccg tttcccctac
      181 tacctctccc ggcaccggct gagccactcg gggctccggc cccacgcctg cccgctgtgc
      241 cccaaggcct tccgccggcc ggcccacctt tcccgccacc tgcgcggcca cgggccccag
      301 cccccgctgc gctgcgccgc ctgcccccgc accttcccag aaccggccca gctcaggcgc
      361 cacctggctc aggagcacgc gggcggcgag gtcgagctgg ccatcgagag ggtggccaag
      421 gagacggccg aacccagctg gggcccgcag gacgagggct cggagccgcc caccaccgct
      481 gcagcagggg ccacggagga ggaggcggtc gcagcgtggc ctgagacgtg gcctgcgggg
      541 gagccttcca cgctggctgc gcccaccagc gccgcggagc cccgggagtc ggagtcggag
      601 gaggccgagg ccggggcagc agagctgagg gccgagctgg cgctggcggc cgggcggcag
      661 gaggagaaac aggtcctgct ccaggcagac tggacgctgc tgtgtctgcg ctgccgcgaa
      721 gccttcgcca ccaagggcga gctcaaggcg cacccgtgtc tgcgccccga gggcgaacag
      781 gaaggggaag gggggccccc gccacgcccc aagcgacacc agtgctccat ctgcctcaag
      841 gccttcgcca ggccctggtc cctgtcgcgc caccggctgg tccactccac cgaccgccct
      901 ttcgtgtgcc cagactgcgg cctggccttc cgcctcgcct cctacctccg ccagcaccgc
      961 cgcgtccacg gccccctcag cctgctggcc ccgctgcccg cggcgggcaa gaaggacgac
     1021 aaggcctcgg gtgcacggaa ctcagccaag gggccggagg ggggcgaggg ggcggagtgc
     1081 gggggtgcct cggaaggggg agaagggcag aacggaggcg acgccgcccc ggctcggccc
     1141 cccgccgggg agccccgctt ctggtgccca gagtgcggca aaggtttcag gcgccgggcg
     1201 cacctgcggc agcacggggt gacccactcg ggagcgcgcc ccttccagtg cgtgcgctgc
     1261 cagcgggagt tcaagcgcct ggccgacctg gcgcgccacg cacaggtgca cgcggggggc
     1321 ccagccccgc acccgtgccc ccgctgcccg cgccgcttct cgcgcgccta cagcctcctg
     1381 cgccaccagc gctgccaccg cgcagagctg gagagggccg ccgcgctgca ggcactgcag
     1441 gcccaggccc cgacgtcgcc gccaccgccc ccgccgcccc tgaaggccga gcaggaggaa
     1501 gaagggctcc cgctgcccct cgcaaacatt aaggaagagc cgccctctcc ggggacccca
     1561 ccccagtccc cgccggctcc ccctgtcttc ctcagcgcct cctgtttcga cagccaagac
     1621 cactcagcct tcgagatgga ggaggaagag gtagacagca aggctcacct gcgcgggctg
     1681 gggggcctgg cctcctagta atgaactagt ccgtaacttg aaagtatttc gatttcttgg
     1741 ctttatatat cttgtggaaa ggacgacttt accgatttat acattcggtt
//
