LOCUS       sequence-284952-        4421 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4421)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 4421)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1225
FEATURES             Location/Qualifiers
     source          1..4421
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggccagctc ggagactgag atccgctggg ctgagcctgg cctggggaag
       61 ggcccccagc ggcggcgctg ggcctgggcc gaggacaaga gggatgtgga tagaagtagt
      121 tcacaaagct gggaagaaga gagactcttt cccaatgcca ccagccccga gctcctagag
      181 gacttccgcc tggcccagca gcacctgccg cccctggagt gggacccaca cccgcagccc
      241 gatgggcatc aggattccga gtcaggagag acttcgggag aagaggctga agcagaggat
      301 gtggacagcc cagcaagttc ccatgagcct cttgcctggc tcccccagca gggccgtcag
      361 ctggacatga ctgaagagga gccagatggg accctcggaa gtctggaggt tgaggaggct
      421 ggagagagct cctcaaggtt ggggtatgag gctggtctca gcttggaagg ccatggaaac
      481 accagcccca tggctcttgg gcatggtcag gccaggggct gggtggcttc tggcgaacaa
      541 gccagtgggg acaaactttc tgaacattcc gaggtcaacc catccgttga actcagcccg
      601 gcaaggtcct ggagcagtgg gacagtgagc ctcgaccacc ctagtgacag ccttgattct
      661 acctgggaag gagagaccga tggcccccag cccactgccc tggcagaaac cttgccagag
      721 ggccccagcc accacctcct aagcccagat ggcagaactg gaggcagtgt tgctcgggca
      781 acccccatgg aattccagga ctcctcagct cccccagccc agagtccgca gcatgccaca
      841 gatagatgga ggagagaaac gaccagattc ttctgccctc agcccaagga acacatctgg
      901 aagcagacaa agacgtcacc taagccactc ccttcccgat tcattggctc catcagcccc
      961 ctgaatcccc agcccaggcc aacgcggcag ggcaggccgc tgcccagaca gggagccact
     1021 ctggctggcc gctcctcttc taatgccccc aagtatggcc gggggcagtt gaactaccca
     1081 ctccctgatt tctccaaggt agggccccgg gtgagattcc ccaaagatga gagctaccgt
     1141 ccccccaagt ccagaagcca caacaggaag cctcaggccc ctgccaggcc cctcatcttc
     1201 aagtctccag ctgagattgt gcaggaggtg ctgttgagca gtggagaagc agccctggca
     1261 aaggacacgc ctcctgccca ccctatcacc agggtacccc aagaatttca gacgcctgag
     1321 caagccactg agctggtcca tcagctccag gaagactacc acaggctcct caccaagtac
     1381 gctgaggccg agaacaccat tgaccagcta cgcctcgggg ccaaggtgaa cctgttctct
     1441 gacccacccc agcccaacca cagcatccac acgggaatgg tgccccaggg gaccaaggtc
     1501 ttgtccttca ccatcccaca gccccgctct gcagagtggt ggccgggccc ggccgaggac
     1561 ccccaggcct ctgcggcctc agggtggcca tcagctcgag gagacttgag cccctcctcg
     1621 cttaccagca tgcccaccct ggggtggctt ccggagaacc gggacatctc tgaggaccag
     1681 tcctcagcag agcagaccca ggcactggct tctcaggcca gccagttcct ggccaaggtg
     1741 gagtcctttg aaagactgat acaggcagga cgtctcatgc cccaggacca agtcaagggc
     1801 ttccagcggc tgaaggctgc ccacgcggcc ctagaggagg agtacctgaa ggcttgtcgg
     1861 gagcaacacc ctgcccagcc gcttgccggc tccaagggga cgcctggaag atttgatcct
     1921 cgcagggagc tggaggcaga gatataccgt ctgggaagct gcctggaaga gctgaaggaa
     1981 cacatagacc agacccagca agagcctgag ccgcccgggt cagactcagc tctggacagc
     2041 accccagccc tgccctgcct ccatcagcca acgcacctgc ctgctccttc tggacaagcc
     2101 cccatgccag ccatcaagac ctcctgccct gagcctgcta ccaccactgc cgccgccagc
     2161 actggcccct gcccattgca cgtaaatgtg gaggtgagct ctggcaacag tgaggtggag
     2221 gacaggccac aggaccccct ggcccgactc aggcacaagg agctgcagat ggagcaagtt
     2281 taccatggcc tcatggagcg gtacctcagt gtgaagtctc tcccagaagc catgagaatg
     2341 gaggaggagg aagaaggaga ggaggaggag gaggaagagg ggggaggtga ctccctggaa
     2401 gttgatgggg tggctgcaac tccagggaaa gcagaggcca ccagggtcct cccaaggcag
     2461 tgcccggtgc aggctgagaa aagtcatggg gctcccctgg aggaggccac ggagaagatg
     2521 gtatctatga agccaccagg tttccaggca tccctggcta gagacgggca catgtcaggc
     2581 ctgggcaagg ctgaggcagc ccctccaggc cctggcgtgc caccccaccc tccaggcacc
     2641 aagtccgcag catcccacca aagtagtatg accagcctgg agggaagcgg catctctgag
     2701 cgccttccac agaagccttt gcaccgaggc ggtgggcccc acctggagga gacctggatg
     2761 gcgtccccag agacagacag tggctttgtg ggctcagaaa caagcagagt ttcacccctc
     2821 acccagactc cagagcaccg gctctcccac atcagcacag caggaacatt agcccagccc
     2881 tttgctgcat ctgtgcccag ggatggagct tcctacccca aggccagggg ttctctgatt
     2941 cccagaagag ccacagagcc cagcacaccc cggagccaag cacagaggta cctctccagc
     3001 ccaagtgggc ctctccggca gagggcaccc aacttcagcc tggagcggac actggcagcc
     3061 gagatggcgg ttcctggctc agagtttgag gggcacaaac ggatttctga acagcccctt
     3121 cccaacaaga caatcagccc acccccagcc cccgcccctg ccgctgcgcc tctaccctgt
     3181 ggaccaacag agaccatccc cagcttcctg ctcaccaggg cagggcgaga ccaggccatc
     3241 tgtgagctgc aagaagaggt gtcccggctt cgtctgcggc tggaagacag cctgcaccag
     3301 ccactccagg gcagcccgac acgcccagca tctgcctttg accgccccgc ccggacccgc
     3361 ggccggccag cagactcccc agccacctgg ggctcccatt atggcagtaa atccacagag
     3421 agattgcctg gtgagcctag aggtgaagag cagattgtcc ctccaggaag gcagcgagcc
     3481 aggtcttcct cagtgcctcg ggaggtgctc cgactgtccc tgagttcaga atctgagctg
     3541 ccctccctac cactgttctc tgagaagagc aagaccacca aggacagtcc acaggcagct
     3601 cgggatggaa agagaggggt gggcagtgct ggatggccag acagggtcac cttccggggc
     3661 caatacacag gccacgaata ccatgttctg tcccctaagg cggtcccaaa aggcaatggc
     3721 acagtctcct gtccccactg ccggcccatt aggacccagg atgcgggtgg tgctgtcaca
     3781 ggggacccac tgggaccgcc tcccgctgat acccttcagt gtcccctgtg tggtcaagtt
     3841 gggtctcccc cagaggcaga tggtccaggc tcagccacct ctggggcaga gaaggccacc
     3901 acgaggagaa aagcatcttc aactcccagc cccaagcaga ggagcaagca ggcggggtcg
     3961 tcgccacgcc caccccccgg actgtggtat ctggcaacag cgcccccagc accagcccct
     4021 ccagcctttg cctacatctc ctcggttccc atcatgcctt atccacctgc cgctgtgtac
     4081 tatgcgcctg caggacctac ctcagcccaa ccagctgcca agtggccgcc cacagcctct
     4141 cccccaccag cccggagaca ccggcactcc atccagctcg acctgggcga cctagaggag
     4201 ctcaacaagg ccctgagccg ggccgtgcag gctgccgaga gcgtccgctc taccaccagg
     4261 cagatgagaa gctcgctgtc agccgacctg cgccaggctc acagcctgcg gggctcctgc
     4321 ctcttctagt aatgaactag tccgtaactt gaaagtattt cgatttcttg gctttatata
     4381 tcttgtggaa aggacgatga ccaatatcga ggtttcgtac c
//
