LOCUS       sequence-284955-        3782 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3782)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 3782)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1231
FEATURES             Location/Qualifiers
     source          1..3782
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(287..298)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     regulatory      611..620
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggacagctt cgacttagcc ctgctccagg aatgggacct cgagtcactg
       61 tgtgtctatg aaccagatag aaatgcatta cggaggaaag aacgagaaag aagaaatcaa
      121 gaaactcaac aggatgatgg cacgtttaat tctagttact ctctcttcag tgagccctac
      181 aagactaaca agggggatga actctccaac cggatccaga acactttagg caattatgat
      241 gaaatgaaag actttttaac tgatagatcc aatcagagtc atctcgttgg agttcccaaa
      301 cctggggttc ctcagactcc tgtgaacaag atcgatgaac attttgttgc agattcaaga
      361 gcccagaacc agccctcgtc tatctgtagc actacaactt ccacaccagc agctgtcccc
      421 gtgcagcaga gtaagagagg cactatgggc tggcagaagg ctgggcaccc accctctgac
      481 ggccaacaga gagcaacaca acagggctct ctcaggacct tgcttggaga tggtgttggc
      541 agacagcagc ctcgggccaa acaagtgtgc aatgtggagg tgggccttca gacccaggag
      601 aggccacctg ccatggcggc caagcacagc agcagcggac actgtgttca gaactttcct
      661 ccatccctag cttcaaaacc cagcctggtc cagcagaaac cgaccgcgta tgtgaggcca
      721 atggacggcc aagatcaggc ccctgatgag tctcctaagc tgaagtcgtc ttcggaaacc
      781 agcgtgcact gcacatcata caggggagtc cctgccagca agccggagcc tgccagagcc
      841 aaggccaagc tctccaagtt cagcatcccc aagcaggggg aggagagtag atctggagaa
      901 accaacagct gtgttgaaga aataatccgg gagatgacct ggcttccacc actttctgct
      961 attcaagcac ctggcaaagt ggaaccaacc aaatttccat ttccaaataa ggactctcag
     1021 cttgtatcct ctggacacaa taatccaaag aaaggtgatg cagagccaga gagtccagac
     1081 aatggcacat cgaatacatc aatgctggaa gatgacctta agctaagcag tgatgaagag
     1141 gagaatgaac agcaggcagc tcagagaacg gctctccgcg ctctctctga cagcgccgtg
     1201 gtccagcagc ccaactgcag aacctcggtg ccttccagca agggcagcag cagcagcagc
     1261 agcagcggca gcagcagctc ctccagcgac tcagagagca gctccggatc tgactcggag
     1321 accgagagca gctccagcga gagtgagggc agcaagcccc cccacttctc cagccccgag
     1381 gctgaaccgg catcctctaa caagtggcag ctggataaat ggctaaacaa agttaatccc
     1441 cacaagcctc ctattctgat ccaaaatgaa agccacgggt cagagagcaa tcagtactac
     1501 aacccggtga aagaggacgt ccaggactgt gggaaagtcc ccgacgtttg ccagcccagc
     1561 ctgagagaga aggagatcaa gagcacttgc aaggaggagc aaaggccaag gacagccaac
     1621 aaggcccctg ggagtaaagg cgtgaagcag aagtccccgc ccgcggccgt ggccgtggcg
     1681 gtgagcgcag ccgccccgcc acccgcagtg ccctgtgcgc ccgcggagaa cgcgcccgcg
     1741 cctgcccgga ggtccgcggg caagaagccc accaggcgca ccgagaggac ctcagccggg
     1801 gacggcgcca actgccaccg gcccgaggag cccgcggccg cggacgcgct ggggacgagc
     1861 gtggtggtcc ccccggagcc caccaaaacc aggccctgtg gcaacaacag agcgagccac
     1921 cgcaaggagc tgcgctcctc cgtgacctgc gagaagcgcc gcacgcgggg gctaagcagg
     1981 atcgtcccca aatccaagga gttcattgag acagagtcgt catcttcatc ctcctcctcg
     2041 gactccgacc tggagtccga gcaggaggag taccctctgt ccaaagcaca gaccgtggct
     2101 gcctctgcct cctccgggaa tgatcagagg ctgaaggagg ccgctgccaa cgggggcagt
     2161 ggtcctaggg cccctgtagg ctccatcaac gccaggacca ccagtgacat cgccaaggag
     2221 ctggaggagc agttctacac actggtcccc tttggccgga acgaacttct ctcccctcta
     2281 aaggacagtg atgagatcag gtctctctgg gtcaaaatcg acctgaccct cctgtccagg
     2341 atcccagaac acctgcccca ggagccaggg gtattgagcg cccctgccac caaggactct
     2401 gagagcgcac cgcccagcca cacctcggac acacctgcag aaaaggcttt gccaaaatcc
     2461 aagaggaaac gcaagtgtga caacgaagac gactacaggg agatcaagaa gtcccaggga
     2521 gagaaagaca gctcttcaag actggccacc tccaccagta atactttgtc tgcaaaccac
     2581 tgcaacatga acatcaacag tgtggcaata ccaataaata aaaatgaaaa aatgcttcgg
     2641 tcgcccatct cacccctctc tgatgcatct aaacacaaat acaccagcga ggacttaact
     2701 tcttccagcc gacctaatgg caacagtttg tttacttcag cctcttccag caaaaagcct
     2761 aaggccgaca gccagctgca gcctcacggc ggagacctca cgaaagcagc tcacaacaat
     2821 tctgaaaaca ttcccctcca caagtcacgg ccgcagacga agccgtggtc tccaggctcc
     2881 aacggccaca gggactgcaa gaggcagaaa cttgtcttcg atgatatgcc tcgcagtgcc
     2941 gattatttta tgcaagaagc taaacgaatg aagcataaag cagatgcaat ggtggaaaag
     3001 tttggaaagg ctttgaacta tgctgaagca gcattgtcgt ttatcgagtg tggaaatgca
     3061 atggaacaag gccccatgga atccaaatct ccttatacga tgtattcaga aacagtagag
     3121 ctcatcaggt atgctatgag actaaaaacc cactcaggcc ccaatgccac accagaagac
     3181 aaacaactgg ctgcattatg ttaccgatgc ctggccctcc tgtactggcg gatgtttcga
     3241 ctcaaaaggg accacgctgt aaagtattca aaagcactaa tcgactattt caagaactca
     3301 tctaaagccg cccaagcccc atctccgtgg ggggccagtg gaaagagcac tggaacccca
     3361 tcccccatgt ctcccaaccc ctctcccgcc agctccgtgg ggtctcaggg cagcctctcc
     3421 aacgccagcg ccctgtcccc gtcgaccatc gtcagcatcc cacagcgcat ccaccagatg
     3481 gcggccaacc acgtcagcat caccaacagc atcctgcaca gctacgacta ctgggagatg
     3541 gccgacaacc tggccaagga aaaccgagaa ttcttcaacg acctggatct gctcatgggg
     3601 ccggtcaccc tgcacagcag catggagcac ctggtccagt actcccaaca gggcctgcac
     3661 tggctgcgga acagcgccca cctgtcatag taatgaacta gtccgtaact tgaaagtatt
     3721 tcgatttctt ggctttatat atcttgtgga aaggacgaca cgcgacaatg tgccaaacat
     3781 cg
//
