LOCUS       sequence-284961-        3758 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3758)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 3758)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1236
FEATURES             Location/Qualifiers
     source          1..3758
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcatttac agaaagagtc aacagcagtg gcaacagttt gtacaatgac
       61 gacagaaacc tgcttcgaat tagagagaag gaaagacgca accaggaagc ccaccaagag
      121 aaagaggcat ttcctgaaaa gattcccctt tttggagagc cctacaagac agcaaaaggt
      181 gatgagctgt ctagtcgaat acagaacatg ttgggaaact acgaagaagt gaaggagttc
      241 cttagtacta agtctcacac tcatcgcctg gatgcttctg aaaataggtt gggaaagccg
      301 aaatatcctt taattcctga caaagggagc agcattccat ccagctcctt ccacactagt
      361 gtccaccacc agtccattca cactcctgcg tctggaccac tttctgttgg caacattagc
      421 cacaatccaa agatggcgca gccaagaact gaaccaatgc caagtctcca tgccaaaagc
      481 tgcggcccac cggacagcca gcacctgacc caggatcgcc ttggtcagga ggggttcggc
      541 tctagtcatc acaagaaagg tgaccgaaga gctgacggag accactgtgc ttcggtgaca
      601 gattcggctc cagagaggga gctttctccc ttaatctctt tgccttcccc agttccccct
      661 ttgtcaccta tacattccaa ccagcaaact cttccccgga cgcaaggaag cagcaaggtt
      721 catggcagca gcaataacag taaaggctat tgcccagcca aatctcccaa ggacctagca
      781 gtgaaagtcc atgataaaga gacccctcaa gacagtttgg tggcccctgc ccagccgcct
      841 tctcagacat ttccacctcc ctccctcccc tcaaaaagtg ttgcaatgca gcagaagccc
      901 acggcttatg tccggcccat ggatggtcaa gatcaggccc ctagtgaatc ccctgaactg
      961 aaaccactgc cggaggacta tcgacagcag acctttgaaa aaacagactt gaaagtgcct
     1021 gccaaagcca agctcaccaa actgaagatg ccttctcagt cagttgagca gacctactcc
     1081 aatgaagtcc attgtgttga agagattctg aaggaaatga cccattcatg gccgcctcct
     1141 ttgacagcaa tacatacgcc tagtacagct gagccatcca agtttccttt ccctacaaag
     1201 gactctcagc atgtcagttc tgtaacccaa aaccaaaaac aatatgatac atcttcaaaa
     1261 actcactcaa attctcagca aggaacgtca tccatgctcg aagacgacct tcagctcagt
     1321 gacagtgagg acagtgacag tgaacaaacc ccagagaagc ctccctcctc atctgcacct
     1381 ccaagtgctc cacagtccct tccagaacca gtggcatcag cacattccag cagtgcagag
     1441 tcagaaagca ccagtgactc agacagttcc tcagactcag agagcgagag cagttcaagt
     1501 gacagcgaag aaaatgagcc cctagaaacc ccagctccgg agcctgagcc tccaacaaca
     1561 aacaaatggc agctggacaa ctggctgacc aaagtcagcc agccagctgc gccaccagag
     1621 ggccccagga gcacagagcc cccacggcgg cacccagaga gtaagggcag cagcgacagt
     1681 gccacgagtc aggagcattc tgaatccaaa gatcctcccc ctaaaagctc cagcaaagcc
     1741 ccccgggccc cacccgaagc cccccacccc ggaaagagga gctgtcagaa gtctccggca
     1801 cagcaggagc ccccacaaag gcaaaccgtt ggaaccaaac aacccaaaaa acctgtcaag
     1861 gcctctgccc gggcaggttc acggaccagc ctgcaggggg aaagggagcc agggcttctt
     1921 ccctatggct cccgagacca gacttccaaa gacaagccca aggtgaagac gaaaggacgg
     1981 ccccgggccg cagcaagcaa cgaacccaag ccagcagtgc ccccctccag tgagaagaag
     2041 aagcacaaga gctccctccc tgccccctct aaggctctct caggcccaga acccgcgaag
     2101 gacaatgtgg aggacaggac ccctgagcac tttgctcttg ttcccctgac tgagagccag
     2161 ggcccacccc acagtggcag cggcagcagg actagtggct gccgccaagc cgtggtggtc
     2221 caggaggaca gccgcaaaga cagactccca ttgcctttga gagacaccaa gctgctctca
     2281 ccgctcaggg acactcctcc cccacaaagc ttgatggtga agatcaccct agacctgctc
     2341 tctcggatac cccagcctcc cgggaagggg agccgccaga ggaaagcaga agataaacag
     2401 ccgcccgcag ggaagaagca cagctctgag aagaggagct cagacagctc aagcaagttg
     2461 gccaaaaaga gaaagggtga agcagaaaga gactgtgata acaagaaaat cagactggag
     2521 aaggaaatca aatcacagtc atcttcatct tcatcctccc acaaagaatc ttctaaaaca
     2581 aagccctcca ggccctcctc acagtcctca aagaaggaaa tgctcccccc gccacccgtg
     2641 tcctcgtcct cccagaagcc agccaagcct gcacttaaga ggtcaaggcg ggaagcagac
     2701 acctgtggcc aggaccctcc caaaagtgcc agcagtacca agagcaacca caaagactct
     2761 tccattccca agcagagaag agtagagggg aagggctcca gaagctcctc ggagcacaag
     2821 ggttcttccg gagatactgc aaatcctttt ccagtgcctt ctttgccaaa tggtaactct
     2881 aaaccaggga agcctcaagt gaagtttgac aaacaacaag cagaccttca catgagggag
     2941 gcaaaaaaga tgaagcagaa agcagagtta atgacggaca gggttggaaa ggcttttaag
     3001 tacctggaag ccgtcttgtc cttcattgag tgcggaattg ccacagagtc tgaaagccag
     3061 tcatccaagt cagcttactc tgtctactca gaaactgtag atctcattaa attcataatg
     3121 tcattaaaat ccttctcaga tgccacagcg ccaacacaag agaaaatatt tgctgtttta
     3181 tgcatgcgtt gccagtccat tttgaacatg gcgatgtttc gttgtaaaaa agacatagca
     3241 ataaagtatt ctcgtactct taataaacac ttcgagagtt cttccaaagt cgcccaggca
     3301 ccttctccat gcattgcaag aagcacaggc acaccatccc ctctttcccc aatgccttct
     3361 cctgccagct ccgtagggtc ccagtcaagt gctggcagtg tggggagcag tggggtggct
     3421 gccactatca gcaccccagt caccatccag aatatgacat cttcctatgt caccatcaca
     3481 tcccatgttc ttaccgcctt tgacctttgg gaacaggccg aggccctcac gaggaagaat
     3541 aaagaattct ttgctcggct cagcacaaat gtgtgcacct tggccctcaa cagcagtttg
     3601 gtggacctgg tgcactatac acgacagggt tttcagcagc tacaagaatt aaccaaaaca
     3661 ccttagtaat gaactagtcc gtaacttgaa agtatttcga tttcttggct ttatatatct
     3721 tgtggaaagg acgacgacat cctaaatcca tggctatc
//
