LOCUS       sequence-284964-        3734 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3734)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 3734)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1237
FEATURES             Location/Qualifiers
     source          1..3734
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcagccca gtcaagtttg tacaatgacg acagaaacct gcttcgaatt
       61 agagagaagg aaagacgcaa ccaggaagcc caccaagaga aagaggcatt tcctgaaaag
      121 attccccttt ttggagagcc ctacaagaca gcaaaaggtg atgagctgtc tagtcgaata
      181 cagaacatgt tgggaaacta cgaagaagtg aaggagttcc ttagtactaa gtctcacact
      241 catcgcctgg atgcttctga aaataggttg ggaaagccga aatatccttt aattcctgac
      301 aaagggagca gcattccatc cagctccttc cacactagtg tccaccacca gtccattcac
      361 actcctgcgt ctggaccact ttctgttggc aacattagcc acaatccaaa gatggcgcag
      421 ccaagaactg aaccaatgcc aagtctccat gccaaaagct gcggcccacc ggacagccag
      481 cacctgaccc aggatcgcct tggtcaggag gggttcggct ctagtcatca caagaaaggt
      541 gaccgaagag ctgacggaga ccactgtgct tcggtgacag attcggctcc agagagggag
      601 ctttctccct taatctcttt gccttcccca gttccccctt tgtcacctat acattccaac
      661 cagcaaactc ttccccggac gcaaggaagc agcaaggttc atggcagcag caataacagt
      721 aaaggctatt gcccagccaa atctcccaag gacctagcag tgaaagtcca tgataaagag
      781 acccctcaag acagtttggt ggcccctgcc cagccgcctt ctcagacatt tccacctccc
      841 tccctcccct caaaaagtgt tgcaatgcag cagaagccca cggcttatgt ccggcccatg
      901 gatggtcaag atcaggcccc tagtgaatcc cctgaactga aaccactgcc ggaggactat
      961 cgacagcaga cctttgaaaa aacagacttg aaagtgcctg ccaaagccaa gctcaccaaa
     1021 ctgaagatgc cttctcagtc agttgagcag acctactcca atgaagtcca ttgtgttgaa
     1081 gagattctga aggaaatgac ccattcatgg ccgcctcctt tgacagcaat acatacgcct
     1141 agtacagctg agccatccaa gtttcctttc cctacaaagg actctcagca tgtcagttct
     1201 gtaacccaaa accaaaaaca atatgataca tcttcaaaaa ctcactcaaa ttctcagcaa
     1261 ggaacgtcat ccatgctcga agacgacctt cagctcagtg acagtgagga cagtgacagt
     1321 gaacaaaccc cagagaagcc tccctcctca tctgcacctc caagtgctcc acagtccctt
     1381 ccagaaccag tggcatcagc acattccagc agtgcagagt cagaaagcac cagtgactca
     1441 gacagttcct cagactcaga gagcgagagc agttcaagtg acagcgaaga aaatgagccc
     1501 ctagaaaccc cagctccgga gcctgagcct ccaacaacaa acaaatggca gctggacaac
     1561 tggctgacca aagtcagcca gccagctgcg ccaccagagg gccccaggag cacagagccc
     1621 ccacggcggc acccagagag taagggcagc agcgacagtg ccacgagtca ggagcattct
     1681 gaatccaaag atcctccccc taaaagctcc agcaaagccc cccgggcccc acccgaagcc
     1741 ccccaccccg gaaagaggag ctgtcagaag tctccggcac agcaggagcc cccacaaagg
     1801 caaaccgttg gaaccaaaca acccaaaaaa cctgtcaagg cctctgcccg ggcaggttca
     1861 cggaccagcc tgcaggggga aagggagcca gggcttcttc cctatggctc ccgagaccag
     1921 acttccaaag acaagcccaa ggtgaagacg aaaggacggc cccgggccgc agcaagcaac
     1981 gaacccaagc cagcagtgcc cccctccagt gagaagaaga agcacaagag ctccctccct
     2041 gccccctcta aggctctctc aggcccagaa cccgcgaagg acaatgtgga ggacaggacc
     2101 cctgagcact ttgctcttgt tcccctgact gagagccagg gcccacccca cagtggcagc
     2161 ggcagcagga ctagtggctg ccgccaagcc gtggtggtcc aggaggacag ccgcaaagac
     2221 agactcccat tgcctttgag agacaccaag ctgctctcac cgctcaggga cactcctccc
     2281 ccacaaagct tgatggtgaa gatcacccta gacctgctct ctcggatacc ccagcctccc
     2341 gggaagggga gccgccagag gaaagcagaa gataaacagc cgcccgcagg gaagaagcac
     2401 agctctgaga agaggagctc agacagctca agcaagttgg ccaaaaagag aaagggtgaa
     2461 gcagaaagag actgtgataa caagaaaatc agactggaga aggaaatcaa atcacagtca
     2521 tcttcatctt catcctccca caaagaatct tctaaaacaa agccctccag gccctcctca
     2581 cagtcctcaa agaaggaaat gctccccccg ccacccgtgt cctcgtcctc ccagaagcca
     2641 gccaagcctg cacttaagag gtcaaggcgg gaagcagaca cctgtggcca ggaccctccc
     2701 aaaagtgcca gcagtaccaa gagcaaccac aaagactctt ccattcccaa gcagagaaga
     2761 gtagagggga agggctccag aagctcctcg gagcacaagg gttcttccgg agatactgca
     2821 aatccttttc cagtgccttc tttgccaaat ggtaactcta aaccagggaa gcctcaagtg
     2881 aagtttgaca aacaacaagc agaccttcac atgagggagg caaaaaagat gaagcagaaa
     2941 gcagagttaa tgacggacag ggttggaaag gcttttaagt acctggaagc cgtcttgtcc
     3001 ttcattgagt gcggaattgc cacagagtct gaaagccagt catccaagtc agcttactct
     3061 gtctactcag aaactgtaga tctcattaaa ttcataatgt cattaaaatc cttctcagat
     3121 gccacagcgc caacacaaga gaaaatattt gctgttttat gcatgcgttg ccagtccatt
     3181 ttgaacatgg cgatgtttcg ttgtaaaaaa gacatagcaa taaagtattc tcgtactctt
     3241 aataaacact tcgagagttc ttccaaagtc gcccaggcac cttctccatg cattgcaagc
     3301 acaggcacac catcccctct ttccccaatg ccttctcctg ccagctccgt agggtcccag
     3361 tcaagtgctg gcagtgtggg gagcagtggg gtggctgcca ctatcagcac cccagtcacc
     3421 atccagaata tgacatcttc ctatgtcacc atcacatccc atgttcttac cgcctttgac
     3481 ctttgggaac aggccgaggc cctcacgagg aagaataaag aattctttgc tcggctcagc
     3541 acaaatgtgt gcaccttggc cctcaacagc agtttggtgg acctggtgca ctatacacga
     3601 cagggttttc agcagctaca agaattaacc aaaacacctt agtaatgaac tagtccgtaa
     3661 cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga cattgggcat
     3721 ccactgagct gagc
//
