LOCUS       sequence-284979-        2150 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2150)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2150)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1268
FEATURES             Location/Qualifiers
     source          1..2150
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggagttagg ctcgcgccgg cgcagtgtcg gctgccggtg ccgcggcctt
       61 tgtctcgcag tcaggaggga gcaagtgacg tttgaggacg tggtagtggg cttcagccag
      121 gaggagtggg ggcagctgaa gcctgcccag aggaccctgt accgtgatgt aatgctggac
      181 accttcaggc ttctggtctc tgtgggacat tggttaccga agccgaatgt catctccctg
      241 ctggagcaag aggcagagct gtgggcggtg gagtctagac ttccccaagg cgtgtaccca
      301 gagattaagg gacatttcca gtttttgctt ctttcagact tggaaactag acccaaagtc
      361 aaactgtcag ttctaaagca aggcatctct gaagaaatat ccaacagtgt catcttggta
      421 gaaagattcc tgtgggatgg tctgtggtac tgcaggggtg aggacactga gggccactgg
      481 gaatggagtt gtgagagtct agagagcctg gcagtgccgg tggccttcac gcctgtgaag
      541 acgcctgttc tggagcagtg gcagaggaat gggtttgggg aaaacataag tctgaaccct
      601 gatctcccac atcaaccaat gactcctgaa agacaaagcc cccacacatg gggaacacgt
      661 ggaaaaaggg agaagccaga cctaaatgtt ttacagaaaa cctgtgtaaa agagaaaccc
      721 tacaaatgtc aggaatgcgg aaaggccttt agtcacagct cagcacttat cgaacaccac
      781 cggacgcaca caggagagag accttacgaa tgtcacgaat gcttaaaagg cttccggaac
      841 agctcggcac ttaccaaaca ccagagaatc catactgggg agaaacccta taaatgcact
      901 cagtgtggga ggaccttcaa ccaaattgcc ccactgatcc agcaccagag aactcacaca
      961 ggtgagaagc cctatgaatg cagcgaatgt gggaaatcct tcagttttag gtcctccttc
     1021 agccagcacg agcgaactca cacaggcgag aagccctacg agtgcagtga gtgtgggaaa
     1081 gccttccggc aaagcatcca cctcacccag catctgcgaa tccacactgg ggagaaaccc
     1141 tatcagtgtg gtgagtgtgg caaggccttc agccacagct catccttgac caaacaccag
     1201 cgaatccaca caggggagaa gccctacgag tgccatgagt gtggaaaagc cttcacccag
     1261 atcacaccac tgattcagca ccagaggacc cacacaggag aaaagcccta tgagtgtggt
     1321 gagtgtggga aagccttcag tcagagcaca ctcctgaccg agcatcggag gattcacaca
     1381 ggagagaagc cctatggatg caacgagtgt gggaaaacct tcagccacag ctcctcactc
     1441 agccagcatg agcggacaca cacaggagag aagccctatg agtgcagtca gtgtgggaag
     1501 gccttccggc agagcacaca cctcacccaa caccagcgaa tccacacagg ggagaagccc
     1561 tatgaatgca atgactgcgg caaggcattc agtcacagct cgtccctcac caaacatcag
     1621 cgaatccaca ctggggagaa gccctacgaa tgcaaccagt gtggcagagc cttcagccag
     1681 cttgctcccc tcattcagca tcagaggatc cacacaggag agaaacccta tgaatgtaac
     1741 cagtgtggca gagccttcag ccagagctcc cttctcatcg aacaccagag gattcacacc
     1801 aaggaaaagc cgtatgggtg caatgagtgt gggaaatcct tcagccacag ctcctcgctc
     1861 agccagcacg aaaggacgca cactggggaa aagccctatg agtgtcacga ttgcggaaag
     1921 tcctttaggc agagcaccca cctcactcag caccggagga tccacacagg agagaagcca
     1981 tatgcatgca gggactgtgg aaaggccttt acccacagct cctcccttac caagcaccag
     2041 agaactcaca ctggatagta atgaactagt ccgtaacttg aaagtatttc gatttcttgg
     2101 ctttatatat cttgtggaaa ggacgagatg acccgggcga tcgagaataa
//
