LOCUS       sequence-284982-        1793 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1793)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1793)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1285
FEATURES             Location/Qualifiers
     source          1..1793
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tgggaccact gacaattagg gatgtgactg tagaattctc tctggaggag
       61 tggcattgcc tggacactgc acagcagaat ttatataggg atgtgatgtt agagaactac
      121 agaaacctgg tcttcctggg tattgctgtc tctaagccag acctgatcac ctgtctggag
      181 caaggaaaag agccctgtaa tatgaagaga catgagatgg tagccaaacc cccagttatg
      241 tgttctcata ttgctgaaga cctttgccca gagcgagaca taaaatattt tttccaaaaa
      301 gtcatactga ggagatatga taaatgtgaa catgagaatt tacaattaag aaagggctgt
      361 aaaagtgtgg atgagtgtaa ggtgtgcaaa ggaggttata atggacttaa ccaatgtctg
      421 ataactaccc agagcaaaat gtatcaatgt gataaatatg taaaagtctt ctataagttt
      481 tcaaattcag atagacataa gataagacat actgaaaaga aaacttgcaa atgtaaagaa
      541 tgtggcaaat cattttgcat gctttcacaa ctaactcgac ataagagaat tcatattaga
      601 gagaattccc acaaatgtga agaatgtggc aaagccttta accagtcctc agctcttact
      661 cgacataaga tgactcatac tggagagaaa ccctacaaat gtgaagagtg tggaaaagct
      721 tttaaccggt cttcacacct tactcaacat aaggtaattc atactagaga gaaaccctac
      781 aaatgtgaag agtgtggcaa agcctttaac cggtcttcac acattactca acataagaga
      841 attcataata gagagaagcc cttcaaatat gatgaatgtt gcaaagcctt taagtggtcc
      901 tcagctctta ctacccttac tcaacataag agaattcata ctggagagaa accctacaaa
      961 tgtgaagagt gtggcaaagc ctttaaccag tcctcagccc ttactcgaca taagatgatt
     1021 catactggag agaaaccctt ccaatgtgaa gagtgtggca aagcttttaa ccggtcttca
     1081 caccttactc aacataagat aattcatact aaagagaaac cctacaaatg tgaagagtgt
     1141 ggaaaagcct ttaaccggtc ttcacacctt actaaacata agagaattca tactagagag
     1201 aaggcctaca aatgtgatga atattgcaaa gcttttaact ggtcctcagc tcttactacc
     1261 cttactcagc ataagataat tcatactgga gagaaaccct acaaatgtga agagtgtggc
     1321 aaagccttta accggtcttc ataccttatt cgacataaga taattcatac tggagagaaa
     1381 ccctacaaat gtgaagagtg tggcaaagcc tttaaccagt cttcacacct tactcaacat
     1441 aaaataattc atactgggga gaagccctac aaatgtgaag aatgtggcaa agcctttaac
     1501 cggtcttcac acctttctca acataagata attcatactg gagagaaacc ctacaaatgt
     1561 gaagaatgtg gcaagccttt taatcgtttc tcatacctta ccgtacataa gagaattcat
     1621 gctggagaga accccaacaa atatgaagaa tgtggcaaag cttgtaacca ttcctcaaac
     1681 cttactaaac ataattcata gtaatgaact agtccgtaac ttgaaagtat ttcgatttct
     1741 tggctttata tatcttgtgg aaaggacgag gtcgatcgtc gcccatgcat caa
//
